{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,19]],"date-time":"2026-01-19T14:32:09Z","timestamp":1768833129099,"version":"3.49.0"},"reference-count":33,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,3,10]],"date-time":"2023-03-10T00:00:00Z","timestamp":1678406400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100006928","name":"Ohio State University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006928","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Molecular Biology"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The corn planthopper,\n                    <jats:italic>Peregrinus maidis<\/jats:italic>\n                    , is a vector of several maize viruses and is consequently a significant agricultural pest in many tropical and subtropical regions. As\n                    <jats:italic>P. maidis<\/jats:italic>\n                    has developed resistance to insecticides, the aim of this study was to develop transgenic\n                    <jats:italic>P. maidis<\/jats:italic>\n                    strains that could be used for future genetic biocontrol programs. To facilitate the identification of transgenic\n                    <jats:italic>P. maidis<\/jats:italic>\n                    , we isolated and characterized the promoters for the\n                    <jats:italic>P. maidis<\/jats:italic>\n                    <jats:italic>ubiquitin\u2010like<\/jats:italic>\n                    and\n                    <jats:italic>profilin<\/jats:italic>\n                    genes. Transient expression assays with\n                    <jats:italic>P. maidis<\/jats:italic>\n                    embryos showed that both promoters were active. Transgenic lines were established using\n                    <jats:italic>piggyBac<\/jats:italic>\n                    vectors and fluorescent protein marker genes. The lines carried an auto\u2010regulated\n                    <jats:italic>tetracycline<\/jats:italic>\n                    <jats:italic>transactivator<\/jats:italic>\n                    (\n                    <jats:italic>tTA<\/jats:italic>\n                    ) gene, which has been widely used to establish conditional lethal strains in other insect species. The transgenic lines showed low levels of\n                    <jats:italic>tTA<\/jats:italic>\n                    expression but were viable on diet with or without doxycycline, which inhibits the binding of\n                    <jats:italic>tTA<\/jats:italic>\n                    to DNA. We discuss possible modifications to the\n                    <jats:italic>tTA<\/jats:italic>\n                    overexpression system that could lead to the successful development of conditional lethal strains. To our knowledge, this is the first report of a transgenic Hemiptera. The approach we have taken could potentially be applied to other Hemiptera and, for\n                    <jats:italic>P. maidis,<\/jats:italic>\n                    the technology will facilitate future functional genomics studies.\n                  <\/jats:p>","DOI":"10.1111\/imb.12836","type":"journal-article","created":{"date-parts":[[2023,2,24]],"date-time":"2023-02-24T03:03:48Z","timestamp":1677207828000},"page":"363-375","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Development of transgenic corn planthopper\n                    <scp>\n                      <i>Peregrinus maidis<\/i>\n                    <\/scp>\n                    that express the\n                    <scp>\n                      <i>tetracycline transactivator<\/i>\n                    <\/scp>"],"prefix":"10.1111","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1767-7304","authenticated-orcid":false,"given":"Anandrao A.","family":"Patil","sequence":"first","affiliation":[{"name":"Department of Entomology and Plant Pathology North Carolina State University  Raleigh North Carolina USA"},{"name":"Department of Biology University of Washington  Seattle Washington USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William","family":"Klobasa","sequence":"additional","affiliation":[{"name":"Department of Entomology and Plant Pathology North Carolina State University  Raleigh North Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dina","family":"Espinoza\u2010Rivera","sequence":"additional","affiliation":[{"name":"Department of Entomology and Plant Pathology North Carolina State University  Raleigh North Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4644-5086","authenticated-orcid":false,"given":"Oliver","family":"Baars","sequence":"additional","affiliation":[{"name":"Department of Entomology and Plant Pathology North Carolina State University  Raleigh North Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2318-1388","authenticated-orcid":false,"given":"Marc\u00e9 D.","family":"Lorenzen","sequence":"additional","affiliation":[{"name":"Department of Entomology and Plant Pathology North Carolina State University  Raleigh North Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6536-4735","authenticated-orcid":false,"given":"Maxwell J.","family":"Scott","sequence":"additional","affiliation":[{"name":"Department of Entomology and Plant Pathology North Carolina State University  Raleigh North Carolina 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