{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T12:05:41Z","timestamp":1786622741225,"version":"build-2736575974"},"reference-count":86,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2023,7,18]],"date-time":"2023-07-18T00:00:00Z","timestamp":1689638400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32170521"],"award-info":[{"award-number":["32170521"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Molecular Biology"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Alpha\u2010ketoglutarate\u2010dependent dioxygenase ALKB homologue 4 (ALKBH4) is a member of the Fe (II) and 2\u2010oxoglutarate\u2010dependent ALKB homologue family that plays important roles in epigenetic regulation by alkyl lesions removal in mammals. However, the roles of\n                    <jats:italic>ALKBH4<\/jats:italic>\n                    in insects are not clear. Here,\n                    <jats:italic>TcALKBH4<\/jats:italic>\n                    was cloned and functionally characterised in\n                    <jats:italic>Tribolium castaneum.<\/jats:italic>\n                    Temporal expression revealed that\n                    <jats:italic>TcALKBH4<\/jats:italic>\n                    was highly expressed in early embryos and early pupae. Spatial expression showed that\n                    <jats:italic>TcALKBH4<\/jats:italic>\n                    was highly expressed in the adult testis, and followed by the ovary. RNA interference targeting\n                    <jats:italic>TcALKBH4<\/jats:italic>\n                    at different developmental stages in\n                    <jats:italic>T. castaneum<\/jats:italic>\n                    led to apparent phenotypes including the failure of development in larvae, the reduction of food intake and the deficiency of fertility in adult. However, further dot blot analyses showed that\n                    <jats:italic>TcALKBH4<\/jats:italic>\n                    RNAi does not seem to influence 6\u2009mA levels in vivo. qRT\u2010PCR was used to further explore the underlying molecular mechanisms; the result showed that\n                    <jats:italic>TcALKBH4<\/jats:italic>\n                    mediates the development of larvae possibly through 20E signalling pathway, and the fertility of female and male adult might be regulated by the expression of vitellogenesis and JH signalling pathway, respectively. Altogether, these findings will provide new insights into the potential function of\n                    <jats:italic>ALKBH4<\/jats:italic>\n                    in insects.\n                  <\/jats:p>","DOI":"10.1111\/imb.12865","type":"journal-article","created":{"date-parts":[[2023,7,18]],"date-time":"2023-07-18T07:11:25Z","timestamp":1689664285000},"page":"676-688","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Functional characterisation of Fe (\n                    <scp>II<\/scp>\n                    ) and\n                    <scp>2OG<\/scp>\n                    \u2010dependent dioxygenase\n                    <i>TcALKBH4<\/i>\n                    in the red flour beetle,\n                    <i>Tribolium castaneum<\/i>"],"prefix":"10.1111","volume":"32","author":[{"given":"Runnan","family":"Yu","sequence":"first","affiliation":[{"name":"Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences Nanjing Normal University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences Nanjing Normal University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanxiao","family":"Li","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences Nanjing Normal University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Tang","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences Nanjing Normal University  Nanjing 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