{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T13:53:36Z","timestamp":1762869216153,"version":"build-2065373602"},"reference-count":51,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T00:00:00Z","timestamp":1696982400000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T00:00:00Z","timestamp":1696982400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100005825","name":"National Institute of Food and Agriculture","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006222","name":"United States - India Educational Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006222","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Molecular Biology"],"published-print":{"date-parts":[[2024,4]]},"abstract":"Abstract<\/jats:title>\n \n Drosophila suzukii<\/jats:italic>\n (Matsumura) (Diptera: Drosophilidae), commonly called spotted wing Drosophila, is an important agricultural pest recognised worldwide.\n D. suzukii<\/jats:italic>\n is a pest of soft\u2010skinned fruits as females can lay eggs in ripening fruit before harvest. While strains for genetic biocontrol of\n D. suzukii<\/jats:italic>\n have been made, the development of transgenic\n D. suzukii<\/jats:italic>\n strains and their further screening remain a challenge partly due to the lack of phenotypically trackable genetic\u2010markers, such as those widely used with the model genetic organism\n D. melanogaster<\/jats:italic>\n . Here, we have used CRISPR\/Cas9 to introduce heritable mutations in the eye colour genes\n white<\/jats:italic>\n ,\n cinnabar<\/jats:italic>\n and\n sepia<\/jats:italic>\n , which are located on the X, second and third chromosomes, respectively. Strains were obtained, which were homozygous for a single mutation. Genotyping of the established strains showed insertion and\/or deletions (indels) at the targeted sites. A strain homozygous for mutations in\n cinnabar<\/jats:italic>\n and\n sepia<\/jats:italic>\n showed a pale\u2010yellow eye colour at eclosion but darkened to a sepia colour after a week. The fecundity and fertility of some of the\n cinnabar<\/jats:italic>\n and\n sepia<\/jats:italic>\n strains were comparable with the wild type. Although\n white<\/jats:italic>\n mutant males were previously reported to be sterile, we found that sterility is not fully penetrant and we have been able to maintain white\u2010eyed strains for over a year. The\n cinnabar<\/jats:italic>\n ,\n sepia<\/jats:italic>\n and\n white<\/jats:italic>\n mutant strains developed in this study should facilitate future genetic studies in\n D. suzukii<\/jats:italic>\n and the development of strains for genetic control of this pest.\n <\/jats:p>","DOI":"10.1111\/imb.12879","type":"journal-article","created":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T01:33:41Z","timestamp":1697074421000},"page":"91-100","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Expansion of the genetic toolbox for manipulation of the global crop pest\n \n Drosophila suzukii<\/i>\n <\/scp>\n : Isolation and assessment of eye colour mutant strains"],"prefix":"10.1111","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1695-6686","authenticated-orcid":false,"given":"Amarish K.","family":"Yadav","sequence":"first","affiliation":[{"name":"Department of Entomology and Plant Pathology North Carolina State University Raleigh North Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0457-8120","authenticated-orcid":false,"given":"Ramasamy","family":"Asokan","sequence":"additional","affiliation":[{"name":"ICAR\u2010Indian Institute of Horticultural Research Bengaluru Karnataka India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akihiko","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Department of Entomology and Plant Pathology North Carolina State University Raleigh North Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1767-7304","authenticated-orcid":false,"given":"Anandrao A.","family":"Patil","sequence":"additional","affiliation":[{"name":"Department of Entomology and Plant Pathology North Carolina State University Raleigh North Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6536-4735","authenticated-orcid":false,"given":"Maxwell J.","family":"Scott","sequence":"additional","affiliation":[{"name":"Department of Entomology and Plant Pathology North Carolina State University Raleigh North Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,10,11]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12863-020-00940-5"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes13050737"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10340-015-0681-z"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2013.2840"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmicrobiol.2017.92"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1713139115"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnmol.2022.1110538"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/arch.21439"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1400380402"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587-019-0032-3"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tig.2022.10.007"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-59745-583-1_5"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.4497"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/19.18.5082"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/jee\/toac118"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/jee\/toac052"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/iyac035"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.5240"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgg.2016.04.008"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004270000110"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/eea.12599"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1089\/crispr.2021.0032"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1089\/genbio.2022.0019"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20060424"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.6110"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz365"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/aesa\/sau014"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2015.12.081"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.6530"},{"volume-title":"The 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