{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,26]],"date-time":"2025-11-26T16:48:22Z","timestamp":1764175702695,"version":"build-2065373602"},"reference-count":44,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2024,7,6]],"date-time":"2024-07-06T00:00:00Z","timestamp":1720224000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31970476","31772517"],"award-info":[{"award-number":["31970476","31772517"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003399","name":"Science and Technology Commission of Shanghai Municipality","doi-asserted-by":"publisher","award":["18140902200"],"award-info":[{"award-number":["18140902200"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Molecular Biology"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Insect reproductive capacity can affect effective pest control and infertility studies and has become an important focus in recent molecular genetic research. Nucleosome assembly protein (\n                    <jats:italic>Nap<\/jats:italic>\n                    ) is highly conserved across multiple species and is involved in forming the sperm nucleus in many species. We used clustered regularly interspaced palindromic repeats\/Cas9 technology to knockout\n                    <jats:italic>BmNap<\/jats:italic>\n                    in\n                    <jats:italic>Bombyx mori<\/jats:italic>\n                    and observed that the mutations caused female infertility, whereas male fertility was not affected.\n                    <jats:italic>BmNap<\/jats:italic>\n                    mutants grew and mated normally; however, female mutants laid smaller eggs that could not be fertilised and did not hatch. In addition, female sterility produced by the mutation could be inherited stably via male mutants; therefore,\n                    <jats:italic>Nap<\/jats:italic>\n                    could be used as a potential target for lepidopteran pest control through population regulation. In the current study, we elucidated a new function of\n                    <jats:italic>BmNap<\/jats:italic>\n                    , increased the understanding of the oogenesis regulation network in Lepidoptera and promoted the development of insect sterility technologies.\n                  <\/jats:p>","DOI":"10.1111\/imb.12937","type":"journal-article","created":{"date-parts":[[2024,7,6]],"date-time":"2024-07-06T04:13:30Z","timestamp":1720239210000},"page":"722-731","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["<scp>CRISPR<\/scp>\n                    \/\n                    <scp>Cas9<\/scp>\n                    \u2010mediated\n                    <i>Nap<\/i>\n                    knockout affects female reproduction and egg shape in\n                    <i>Bombyx mori<\/i>"],"prefix":"10.1111","volume":"33","author":[{"given":"Xingyu","family":"Liu","sequence":"first","affiliation":[{"name":"School of Life Sciences East China Normal University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liying","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Life Sciences East China Normal University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ning","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Life Sciences East China Normal University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kai","family":"Li","sequence":"additional","affiliation":[{"name":"School of Life Sciences East China Normal University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter B.","family":"Mater","sequence":"additional","affiliation":[{"name":"Faculty of Science Queensland University of Technology  Brisbane Queensland 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