{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T19:58:35Z","timestamp":1787169515968,"version":"build-2736575974"},"reference-count":20,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,8,11]],"date-time":"2024-08-11T00:00:00Z","timestamp":1723334400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BBS\/E\/I\/00007033"],"award-info":[{"award-number":["BBS\/E\/I\/00007033"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BBS\/E\/I\/00007038"],"award-info":[{"award-number":["BBS\/E\/I\/00007038"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BBS\/E\/I\/00007039"],"award-info":[{"award-number":["BBS\/E\/I\/00007039"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Molecular Biology"],"published-print":{"date-parts":[[2025,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Anopheles stephensi<\/jats:italic>\n                    Liston, 1901 (Diptera: culicidae) is a competent vector of\n                    <jats:italic>Plasmodium falciparum<\/jats:italic>\n                    (Haemosporida: plasmodiidae) malaria, and its expansion in the African continent is of concern due to its viability in urban settings and resistance to insecticides. To enhance its genetic tractability, we determined the utility of a ~2\u2009kb\n                    <jats:italic>An. stephensi lipophorin<\/jats:italic>\n                    (\n                    <jats:italic>lp<\/jats:italic>\n                    ) promoter fragment in driving transgene expression. Lipophorin genes are involved in lipid transport in insects, and an orthologous promoter in\n                    <jats:italic>An. gambiae<\/jats:italic>\n                    (AGAP001826) was previously demonstrated to successfully express a transgene. In the present study, we qualitatively characterised the expression of a ZsYellow fluorescent marker protein, expressed by\n                    <jats:italic>An. stephensi lp<\/jats:italic>\n                    promoter fragment. Our study indicated that the\n                    <jats:italic>lp<\/jats:italic>\n                    promoter fragment was effective, generating a distinct expression pattern in comparison to the commonly utilised 3xP3 promoter. The\n                    <jats:italic>lp<\/jats:italic>\n                    :ZsYellow fluorescence was largely visible in early instar larvae and appeared more intense in later instar larvae, pupae and adults, becoming especially conspicuous in adult females after a blood meal. Different isolines showed some variation in expression pattern and intensity. Aside from general transgene expression, as the\n                    <jats:italic>lp<\/jats:italic>\n                    promoter produces a suitable fluorescent protein marker expression pattern, it may facilitate genotypic screening and aid the development of more complex genetic biocontrol systems, such as multi\u2010component gene drives. This study represents an expansion of the\n                    <jats:italic>An. stephensi<\/jats:italic>\n                    genetic toolbox, an important endeavour to increase the speed of\n                    <jats:italic>An. stephensi<\/jats:italic>\n                    research and reach public health milestones in combating malaria.\n                  <\/jats:p>","DOI":"10.1111\/imb.12953","type":"journal-article","created":{"date-parts":[[2024,8,11]],"date-time":"2024-08-11T23:23:42Z","timestamp":1723418622000},"page":"104-110","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Expanding the transgene expression toolbox of the malaria vector\n                    <i>Anopheles stephensi<\/i>"],"prefix":"10.1111","volume":"34","author":[{"given":"Joshua","family":"Southworth","sequence":"first","affiliation":[{"name":"Department of Biosciences Durham University  Durham UK"},{"name":"Arthropod Genetics The Pirbright Institute  Pirbright UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Estela","family":"Gonzalez","sequence":"additional","affiliation":[{"name":"Arthropod Genetics The Pirbright Institute  Pirbright UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherine","family":"Nevard","sequence":"additional","affiliation":[{"name":"Arthropod Genetics The Pirbright Institute  Pirbright UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mireia","family":"Larrosa\u2010Godall","sequence":"additional","affiliation":[{"name":"Arthropod Genetics The Pirbright Institute  Pirbright UK"},{"name":"Department of Biology University of York  York UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luke","family":"Alphey","sequence":"additional","affiliation":[{"name":"Arthropod Genetics The Pirbright Institute  Pirbright UK"},{"name":"Department of Biology University of York  York UK"},{"name":"York Biomedical Research Institute University of York  York UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1510-2942","authenticated-orcid":false,"given":"Michelle A. E.","family":"Anderson","sequence":"additional","affiliation":[{"name":"Arthropod Genetics The Pirbright Institute  Pirbright UK"},{"name":"Department of Biology University of York  York UK"},{"name":"York Biomedical Research Institute University of York  York UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,8,11]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-021-05026-4"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aac7932"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.nutr.21.1.23"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2018.09.001"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1152"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/PNAS.95.7.3748\/ASSET\/DDC440C4\u20105C11\u20104DDA\u2010890D\u201051204E127E7D\/ASSETS\/GRAPHIC\/PQ0584396003.JPEG"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.ACTATROPICA.2014.06.016"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.7554\/ELIFE.93142"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1046\/J.1365-2583.1999.00139.X"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2022.11.011"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2301666120"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpb.2006.01.012"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.3201\/EID1302.060631"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1073\/PNAS.1716358116"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.200397611"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/J.1744-7917.2010.01316.X"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1534\/g3.115.016808"},{"key":"e_1_2_11_19_1","volume-title":"World malaria report 2022","author":"World Health Organization","year":"2022"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-020-03252-2"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-022-03215-0"}],"container-title":["Insect Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/resjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/imb.12953","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T08:42:18Z","timestamp":1762072938000},"score":1,"resource":{"primary":{"URL":"https:\/\/resjournals.onlinelibrary.wiley.com\/doi\/10.1111\/imb.12953"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,11]]},"references-count":20,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,2]]}},"alternative-id":["10.1111\/imb.12953"],"URL":"https:\/\/doi.org\/10.1111\/imb.12953","archive":["Portico"],"relation":{},"ISSN":["0962-1075","1365-2583"],"issn-type":[{"value":"0962-1075","type":"print"},{"value":"1365-2583","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8,11]]},"assertion":[{"value":"2024-01-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-07-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-08-11","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}