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Recent studies have expanded to agricultural pests such as fall armyworm (FAW), scientifically known as\n                    <jats:italic>Spodoptera frugiperda<\/jats:italic>\n                    J. E. Smith (Lepidoptera: Noctuidae), which significantly threatens global agricultural productivity. However, the specific role of the\n                    <jats:italic>hox<\/jats:italic>\n                    gene\n                    <jats:italic>Sfabd\u2010B<\/jats:italic>\n                    in FAW remains unexplored. This research investigates the spatial and temporal expression patterns of\n                    <jats:italic>Sfabd\u2010B<\/jats:italic>\n                    in various tissues at different developmental stages using quantitative real\u2010time polymerase chain reaction (qRT\u2010PCR). Additionally, we explored the potential function of the\n                    <jats:italic>Sfabd\u2010B<\/jats:italic>\n                    gene located in the FAW genome using CRISPR\/Cas9 technology. The larval mutant phenotypes can be classified into three subgroups as compared with wild\u2010type individuals, that is, an excess of pedis in the posterior abdomen, deficient pedis due to segmental fusion and deviations in the posterior abdominal segments. Importantly, significant differences in mutant phenotypes between male and female individuals were also evident during the pupal and adult phases. Notably, both the\n                    <jats:italic>decapentaplegic<\/jats:italic>\n                    (\n                    <jats:italic>dpp<\/jats:italic>\n                    ) and\n                    <jats:italic>cuticular protein 12<\/jats:italic>\n                    (\n                    <jats:italic>cp 12<\/jats:italic>\n                    ) genes displayed a substantial marked decrease in expression levels in the copulatory organ of male mutants and the ovipositor of female mutants compared with the wild type. These findings highlight the importance of\n                    <jats:italic>Sfabd\u2010B<\/jats:italic>\n                    in genital tract patterning, providing a potential target for improving genetic control.\n                  <\/jats:p>","DOI":"10.1111\/imb.12958","type":"journal-article","created":{"date-parts":[[2024,9,24]],"date-time":"2024-09-24T03:20:22Z","timestamp":1727148022000},"page":"162-173","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["<scp>CRISPR\/Cas9<\/scp>\n                    \u2010mediated knockout of the\n                    <i>abdominal\u2010B<\/i>\n                    homeotic gene in the global pest, fall armyworm (\n                    <i>Spodoptera frugiperda<\/i>\n                    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Agricultural University  Zhengzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qi\u2010Qi","family":"Qiu","sequence":"additional","affiliation":[{"name":"College of Plant Protection Henan Agricultural University  Zhengzhou China"},{"name":"Green Pesticide Creation Engineering Technology Research Center Henan Agricultural University  Zhengzhou China"},{"name":"Key Laboratory of New Pesticide Development and Application Henan Agricultural University  Zhengzhou China"},{"name":"Pesticide Department of the College of Plant Protection Henan Agricultural University  Zhengzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cong\u2010Ke","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Plant Protection Henan Agricultural University  Zhengzhou China"},{"name":"Green Pesticide Creation Engineering Technology Research Center Henan Agricultural University  Zhengzhou China"},{"name":"Key Laboratory of New Pesticide 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