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However, its function in the growth, development and morphogenesis of\n                    <jats:italic>Gryllus bimaculatus<\/jats:italic>\n                    remains poorly understood. This study aimed to explore the function of the\n                    <jats:italic>Scr<\/jats:italic>\n                    gene in\n                    <jats:italic>G<\/jats:italic>\n                    .\n                    <jats:italic>bimaculatus<\/jats:italic>\n                    by using CRISPR\/Cas9 technology to generate an\n                    <jats:italic>Scr<\/jats:italic>\n                    gene knock\u2010out strain. Intercrossing the G\n                    <jats:sub>0<\/jats:sub>\n                    generation knock\u2010out individuals with wild\u2010type individuals yielded the G\n                    <jats:sub>1<\/jats:sub>\n                    generation to screen the mutant strain. It was found that the knock\u2010out of the\n                    <jats:italic>Scr<\/jats:italic>\n                    gene had a severe impact on the growth and development of\n                    <jats:italic>G<\/jats:italic>\n                    .\n                    <jats:italic>bimaculatus<\/jats:italic>\n                    , resulting in high mortality and making it difficult to obtain\n                    <jats:italic>Scr<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mutants. Therefore, heterozygous individuals (\n                    <jats:italic>Scr<\/jats:italic>\n                    <jats:sup>+\/\u2212<\/jats:sup>\n                    ) with 1\u2009bp deleted were obtained for investigation. The results showed that the\n                    <jats:italic>Scr<\/jats:italic>\n                    deletion led to ectopic segment formation in the G\n                    <jats:sub>0<\/jats:sub>\n                    generation. In the G\n                    <jats:sub>2<\/jats:sub>\n                    generation, it was observed that stable\n                    <jats:italic>Scr<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    strains displayed abnormal embryonic development, characterized by enlarged, blackened and lethal eggs during embryogenesis. During the post\u2010embryonic stage,\n                    <jats:italic>Scr<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mutants exhibited abnormalities in body segmentation, particularly in the head\u2010thorax region, resulting in a dorsal ridge structure. Furthermore, some\n                    <jats:italic>Scr<\/jats:italic>\n                    <jats:sup>+\/\u2212<\/jats:sup>\n                    individuals exhibited a dorsal ridge during the nymphal stage. Notably, this characteristic did not persist into the adult stage. Our findings highlight the distinct but crucial roles of the\n                    <jats:italic>Scr<\/jats:italic>\n                    gene in both embryonic and post\u2010embryonic growth and development of\n                    <jats:italic>G<\/jats:italic>\n                    .\n                    <jats:italic>bimaculatus<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1111\/imb.12987","type":"journal-article","created":{"date-parts":[[2025,2,26]],"date-time":"2025-02-26T19:41:37Z","timestamp":1740598897000},"page":"608-618","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Functional exploration of the\n                    <i>Sex combs reduced<\/i>\n                    gene in\n                    <i>Gryllus 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