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To investigate the involvement of the\n                    <jats:italic>T. castaneum TcCYP4C1<\/jats:italic>\n                    gene in detoxification metabolism under high CO\n                    <jats:sub>2<\/jats:sub>\n                    and the protective role of trehalose against hypoxic stress in insects. In the present study, after successfully silencing the\n                    <jats:italic>TcCYP4C1<\/jats:italic>\n                    gene of\n                    <jats:italic>T. castaneum<\/jats:italic>\n                    by double\u2010stranded RNA(dsRNA), the larvae were exposed to 95% CO\n                    <jats:sub>2<\/jats:sub>\n                    . This exposure resulted in a statistically significant increase in larval mortality and a significant elevation in the activity of the carboxylesterase enzyme (CarE). However, a decrease in mortality from 18.15% to 11.24% was observed when larvae were fed trehalose after dsRNA injection. In addition, the gene expression levels of the trehalose metabolism pathway related genes\n                    <jats:italic>TRE1\u20103<\/jats:italic>\n                    ,\n                    <jats:italic>TRE1\u20104<\/jats:italic>\n                    and\n                    <jats:italic>TPS2<\/jats:italic>\n                    were significantly up\u2010regulated after 95% CO\n                    <jats:sub>2<\/jats:sub>\n                    treatment. In summary, the\n                    <jats:italic>TcCYP4C1<\/jats:italic>\n                    gene emerges as a pivotal factor in the adaptive response of\n                    <jats:italic>T. castaneum<\/jats:italic>\n                    to high CO\n                    <jats:sub>2<\/jats:sub>\n                    . Trehalose effectively mitigates the detrimental effects resulting from the silencing of\n                    <jats:italic>TcCYP4C1<\/jats:italic>\n                    and exposure to high CO\n                    <jats:sub>2<\/jats:sub>\n                    stress in\n                    <jats:italic>T. castaneum<\/jats:italic>\n                    . Our findings not only establish a theoretical foundation for the development of novel pesticides tailored for low\u2010oxygen grain storage environments but also inspire innovative, environmentally sustainable pest management strategies in the grain storage sector.\n                  <\/jats:p>","DOI":"10.1111\/imb.12997","type":"journal-article","created":{"date-parts":[[2025,6,28]],"date-time":"2025-06-28T05:18:41Z","timestamp":1751087921000},"page":"729-742","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["<i>TcCYP4C1<\/i>\n                    in combination with trehalose to cope with high\u2010\n                    <scp>\n                      CO\n                      <sub>2<\/sub>\n                    <\/scp>\n                    stress in\n                    <i>Tribolium castaneum<\/i>\n                    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