{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T21:08:01Z","timestamp":1767906481418,"version":"3.49.0"},"reference-count":57,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,5,30]],"date-time":"2025-05-30T00:00:00Z","timestamp":1748563200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32102189"],"award-info":[{"award-number":["32102189"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Molecular Biology"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Mealybugs are highly aggressive pests that infest various plants and cause substantial economic losses. Histone lysine methyltransferases (KMT) are evolutionarily conserved and proposed to be essential in early embryo development in animals. However, few KMTs have been reported in mealybugs. Here, we identified a novel KMT gene,\n                    <jats:italic>PsASH2<\/jats:italic>\n                    , in the cotton mealybug,\n                    <jats:italic>Phenacoccus solenopsis<\/jats:italic>\n                    Tinsley. This gene was highly expressed in the ovary of female adults. Through RNA interference (RNAi) of\n                    <jats:italic>PsASH2<\/jats:italic>\n                    by dsRNA microinjection, we found a reduction in the number of male embryos and total embryos in the ovaries of pregnant females. Continuous downregulation of\n                    <jats:italic>PsASH2<\/jats:italic>\n                    in mated females until their death resulted in few changes in sex ratio but significant decreases in the number of both male and female offspring. Therefore, we believe that\n                    <jats:italic>PsASH2<\/jats:italic>\n                    plays essential roles in embryo survival for both sexes of the cotton mealybug which may provide a potential target gene for the management of cotton mealybug by disrupting embryo development.\n                  <\/jats:p>","DOI":"10.1111\/imb.13003","type":"journal-article","created":{"date-parts":[[2025,5,31]],"date-time":"2025-05-31T00:29:11Z","timestamp":1748651351000},"page":"753-762","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Silencing\n                    <i>PsASH2<\/i>\n                    affects embryo development in the cotton mealybug"],"prefix":"10.1111","volume":"34","author":[{"given":"Kaixin","family":"Wang","sequence":"first","affiliation":[{"name":"College of Life Sciences China Jiliang University  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lingqi","family":"Zen","sequence":"additional","affiliation":[{"name":"College of Life Sciences China Jiliang University  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lilu","family":"Sheng","sequence":"additional","affiliation":[{"name":"College of Life Sciences China Jiliang University  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feihuang","family":"Lu","sequence":"additional","affiliation":[{"name":"College of Life Sciences China Jiliang University  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qianjin","family":"Lin","sequence":"additional","affiliation":[{"name":"College of Life Sciences China Jiliang University  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yifan","family":"Li","sequence":"additional","affiliation":[{"name":"College of Life Sciences China Jiliang University  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7094-2832","authenticated-orcid":false,"given":"Haojie","family":"Tong","sequence":"additional","affiliation":[{"name":"College of Life Sciences China Jiliang University  Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,5,30]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"83","article-title":"Systematic status of a new species of the genus Phenacoccus Cockerell (Pseudococcidae), a serious pest of cotton, Gossypium hirsutum L., in Pakistan","volume":"27","author":"Abbas G.","year":"2005","journal-title":"Pakistan Entomologist"},{"issue":"3","key":"e_1_2_9_3_1","first-page":"421","article-title":"Host plants distribution and overwintering of cotton mealybug (Phenacoccus solenopsis; Hemiptera: Pseudococcidae)","volume":"12","author":"Abbas G.","year":"2010","journal-title":"International Journal of 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