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Nevertheless, the regulatory role of Kr\u2010h1 on vitellogenesis deserves further exploration. Here, we found that\n                    <jats:italic>Kr\u2010h1<\/jats:italic>\n                    was actively expressed from 0\u2010 to 12\u2010day\u2010old females and among detected tissues including fat body and ovary in two potato\u2010defoliating beetles,\n                    <jats:italic>Henosepilachna vigintioctopunctata<\/jats:italic>\n                    and\n                    <jats:italic>H. vigintioctomaculata<\/jats:italic>\n                    . RNA interference (RNAi) for\n                    <jats:italic>Kr\u2010h1<\/jats:italic>\n                    significantly lengthened the preoviposition period, dramatically impaired ovarian development, and decreased oviposition. Weaker yolk accumulation occurred in the\n                    <jats:italic>Kr\u2010h1<\/jats:italic>\n                    RNAi oocytes, in contrast to a larger quantity of yolk granules in controls. Correspondingly,\n                    <jats:italic>Vg<\/jats:italic>\n                    transcript levels in the fat bodies and ovaries and of\n                    <jats:italic>VgR<\/jats:italic>\n                    amounts in the ovarian tissues were substantially reduced in the\n                    <jats:italic>Kr\u2010h1<\/jats:italic>\n                    knockdown samples. In\n                    <jats:italic>H. vigintioctomaculata<\/jats:italic>\n                    , RNAi for the other two JH signal genes,\n                    <jats:italic>HvmHairy<\/jats:italic>\n                    and\n                    <jats:italic>HvmGroucho<\/jats:italic>\n                    , was also performed. Silencing either or both genes affected neither female reproduction nor the mRNA levels of\n                    <jats:italic>Vg<\/jats:italic>\n                    and\n                    <jats:italic>VgR<\/jats:italic>\n                    . Therefore, our findings suggest Kr\u2010h1, rather than Hairy\/Groucho, regulates vitellogenesis in the two\n                    <jats:italic>Henosepilachna<\/jats:italic>\n                    species. Moreover, targeting\n                    <jats:italic>Kr\u2010h1<\/jats:italic>\n                    could potentially be a new way to control potato pests.\n                  <\/jats:p>","DOI":"10.1111\/imb.70022","type":"journal-article","created":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T01:17:24Z","timestamp":1765588644000},"page":"177-186","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Kr\u2010h1 is vital for vitellogenesis in two ladybird beetle species"],"prefix":"10.1111","volume":"35","author":[{"given":"Hai\u2010Hui","family":"Liu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Agricultural and Forestry Biosecurity, College of Plant Protection Nanjing Agricultural University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao\u2010Qing","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural and 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