{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T09:17:39Z","timestamp":1777972659202,"version":"3.51.4"},"reference-count":33,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T00:00:00Z","timestamp":1767916800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T00:00:00Z","timestamp":1767916800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","award":["LZ20C140002"],"award-info":[{"award-number":["LZ20C140002"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32172390"],"award-info":[{"award-number":["32172390"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32472552"],"award-info":[{"award-number":["32472552"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Molecular Biology"],"published-print":{"date-parts":[[2026,6]]},"abstract":"Abstract<\/jats:title>\n \n E75, a member of the nuclear receptor gene family, regulates diverse biological processes, including ovarian development and fat body remodelling. The brown planthopper (\n Nilaparvata lugens<\/jats:italic>\n St\u00e5l) (Hemiptera:Delphacidae), a major rice pest, poses a significant threat to rice production. However, the role of\n Nilaparvata lugens E75<\/jats:italic>\n (\n NlE75<\/jats:italic>\n ) in development, particularly ovarian development and its underlying mechanisms, remains largely unknown. In this study, we found that downregulation of\n NlE75<\/jats:italic>\n expression significantly reduced the eclosion rate, especially in the third\u2010, fourth\u2010 and fifth\u2010instar nymphs. Moreover, decreased\n NlE75<\/jats:italic>\n levels affected fat body morphology as well as ovarian and testicular development, leading to delayed ovarian maturation and reduced egg production.\n NlE75<\/jats:italic>\n knockdown drastically reduced the total number of eggs laid. We also found that\n NlE75<\/jats:italic>\n may participate in the transcriptional regulation of\n Vitellogenin<\/jats:italic>\n (\n Vg<\/jats:italic>\n ),\n Vitellogenin\u2010like1<\/jats:italic>\n (\n Vg\u2010like1<\/jats:italic>\n ) and\n Vitellogenin\u2010like2<\/jats:italic>\n (\n Vg\u2010like2<\/jats:italic>\n ) and that downregulation of\n NlE75<\/jats:italic>\n significantly reduced Vg protein expression. In summary, we found that\n NlE75<\/jats:italic>\n is required for lipid droplet formation in the fat body, as well as for ovary and vas deferens development. Furthermore, downregulation of\n NlE75<\/jats:italic>\n altered fat body morphology and reduced lipid content, suggesting decreased energy reserves. Importantly,\n NlE75<\/jats:italic>\n was found to be involved in the transcriptional regulation of cell cycle\u2010related genes, influencing DNA replication and the expression of genes controlling cell cycle progression. Flow cytometry analysis further revealed significant disruption of ovarian cell cycles following\n NlE75<\/jats:italic>\n downregulation, suggesting that\n E75<\/jats:italic>\n may function in cell cycle progression to coordinate development and reproduction in\n N. lugens<\/jats:italic>\n . The involvement of\n NlE75<\/jats:italic>\n in regulating cell cycle\u2010related genes and cell cycle progression in\n N. lugens<\/jats:italic>\n represents a novel finding. This study provides a valuable reference for related research in other species, offering new insights into the mechanisms of\n NlE75<\/jats:italic>\n function and its interaction with both JH and ecdysone signalling.\n <\/jats:p>","DOI":"10.1111\/imb.70026","type":"journal-article","created":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T15:00:51Z","timestamp":1767970851000},"page":"257-269","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Nuclear receptor\n E75<\/scp>\n regulates moulting and ovarian development in\n Nilaparvata lugens<\/i>"],"prefix":"10.1111","volume":"35","author":[{"given":"Xiaojuan","family":"Jiang","sequence":"first","affiliation":[{"name":"College of Biotechnology and Bioengineering Zhejiang University of Technology Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhenghui","family":"Qiu","sequence":"additional","affiliation":[{"name":"College of Biotechnology and Bioengineering Zhejiang University of Technology Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fuhong","family":"Wei","sequence":"additional","affiliation":[{"name":"College of Biotechnology and Bioengineering Zhejiang University of Technology Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anfu","family":"Bamu","sequence":"additional","affiliation":[{"name":"College of Biotechnology and Bioengineering Zhejiang University of Technology Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huidan","family":"Yuan","sequence":"additional","affiliation":[{"name":"College of Biotechnology and Bioengineering Zhejiang University of Technology Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3981-1818","authenticated-orcid":false,"given":"Xinda","family":"Lin","sequence":"additional","affiliation":[{"name":"College of Biotechnology and Bioengineering Zhejiang University of Technology Hangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,1,9]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2102851118"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1534-5807(02)00204-6"},{"key":"e_1_2_9_4_1","article-title":"Analysis on the key factors causing the outbreak of brown planthopper in Yangtze area, China in 2005","volume":"32","author":"Cheng J.A.","year":"2006","journal-title":"Plant Protection"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/1747-1028-4-3"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2011.11.006"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2004.07.022"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2004.01.009"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.273458"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinsphys.2010.03.016"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M300699200"},{"key":"e_1_2_9_12_1","unstructured":"Jiang X. Qiu Z. Wei F. Bamu A. Yuan H.&Lin X.(2025)Nuclear receptor E75 regulates molting and ovarian development in Nilaparvata lugens. Dryad. Available from:https:\/\/doi.org\/10.5061\/dryad.g1jwstr4w"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep12114"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M116.737072"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-ento-011118-112007"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2024.130607"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2020.101040"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1721473115"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep18064"},{"key":"e_1_2_9_20_1","first-page":"E7738","article-title":"Juvenile hormone\u2010regulated alternative splicing of the Taiman gene primes the ecdysteroid response in adult mosquitoes","volume":"115","author":"Liu P.","year":"2018","journal-title":"Proceedings of the National Academy of Sciences"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.22.2.430-441.2002"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/imb.12635"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi900697c"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0303-7207(99)00022-2"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2005.07.005"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2019.01181"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cois.2018.07.003"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/imb.12365"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2020.593613"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-ento-120811-153608"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0086503"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xpro.2020.100080"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2017.11.001"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1006\/dbio.1997.8798"}],"container-title":["Insect Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/resjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/imb.70026","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/resjournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/imb.70026","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/resjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/imb.70026","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T09:00:45Z","timestamp":1777971645000},"score":1,"resource":{"primary":{"URL":"https:\/\/resjournals.onlinelibrary.wiley.com\/doi\/10.1111\/imb.70026"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,9]]},"references-count":33,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["10.1111\/imb.70026"],"URL":"https:\/\/doi.org\/10.1111\/imb.70026","archive":["Portico"],"relation":{},"ISSN":["0962-1075","1365-2583"],"issn-type":[{"value":"0962-1075","type":"print"},{"value":"1365-2583","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,9]]},"assertion":[{"value":"2025-02-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-08","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}