{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T19:44:44Z","timestamp":1783453484843,"version":"3.55.0"},"reference-count":62,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T00:00:00Z","timestamp":1776124800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T00:00:00Z","timestamp":1776124800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Molecular Biology"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The suppression of Ku70 and Ku80 has been verified to increase homology\u2010directed repair (HDR) efficiency in fruit fly, silkworm and mosquito, but not in other insects. In this study,\n                    <jats:italic>PxKu70<\/jats:italic>\n                    and\n                    <jats:italic>PxKu80<\/jats:italic>\n                    were identified from the\n                    <jats:italic>Plutella xylostella<\/jats:italic>\n                    genome. Domain analysis revealed that PxKu70 contained three conserved domains: Ku N, Ku and Ku C, while PxKu80 comprised the Ku N, Ku and Ku PK bind domains. Phylogenetic analysis and multiple\u2010sequence alignment indicated strong conservation of both proteins among lepidopteran insects. RT\u2010qPCR analysis showed that\n                    <jats:italic>PxKu70<\/jats:italic>\n                    and\n                    <jats:italic>PxKu80<\/jats:italic>\n                    were highly expressed in adult stages, particularly in reproductive tissues such as the ovary and testis, suggesting their role in maintaining genomic stability during gametogenesis. Two homozygous knockout lines (\u0394\n                    <jats:italic>PxKu70<\/jats:italic>\n                    and \u0394\n                    <jats:italic>PxKu80<\/jats:italic>\n                    ) were successfully generated through CRISPR\/Cas9\u2010mediated genome editing. These knockout lines remained viable and fertile without observable fitness effects. A donor construct carrying an\n                    <jats:italic>EGFP<\/jats:italic>\n                    cassette designed for insertion at the\n                    <jats:italic>PxKmo<\/jats:italic>\n                    locus was generated to assess HDR\u2010mediated integration. The HDR insertion rate was significantly elevated in both knockout lines compared with the wild\u2010type. These findings demonstrate that suppression of either\n                    <jats:italic>PxKu70<\/jats:italic>\n                    or\n                    <jats:italic>PxKu80<\/jats:italic>\n                    can enhance HDR in\n                    <jats:italic>P. xylostella<\/jats:italic>\n                    , offering an effective approach for precise genome editing in lepidopteran species.\n                  <\/jats:p>","DOI":"10.1111\/imb.70039","type":"journal-article","created":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T04:13:49Z","timestamp":1776226429000},"page":"434-447","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Knockout of\n                    <i>Ku70<\/i>\n                    and\n                    <i>Ku80<\/i>\n                    elevates homology\u2010directed repair efficiency in\n                    <i>Plutella xylostella<\/i>"],"prefix":"10.1111","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-0053-8452","authenticated-orcid":false,"given":"Faisal","family":"Munir","sequence":"first","affiliation":[{"name":"State Key Laboratory of Agricultural and Forestry Biosecurity, Institute of Applied Ecology Fujian Agriculture and Forestry University  Fuzhou China"},{"name":"Joint International Research Laboratory of Ecological Pest Control Ministry of Education  Fuzhou China"},{"name":"Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross\u2010Strait Crops Fujian Agriculture and Forestry University  Fuzhou China"},{"name":"Key Laboratory of Integrated Pest Management for Fujian\u2010Taiwan Crops Ministry of Agriculture  Fuzhou China"},{"name":"Key Laboratory of Green Pest Control Fujian Province University  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Uroosa","family":"Zaheer","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural and Forestry Biosecurity, Institute of Applied Ecology Fujian Agriculture and Forestry University  Fuzhou China"},{"name":"Joint International Research Laboratory of Ecological Pest Control Ministry of Education  Fuzhou China"},{"name":"Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross\u2010Strait Crops Fujian Agriculture and Forestry University  Fuzhou China"},{"name":"Key Laboratory of Integrated Pest Management for Fujian\u2010Taiwan Crops Ministry of Agriculture  Fuzhou China"},{"name":"Key Laboratory of Green Pest Control Fujian Province University  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad","family":"Asad","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural and Forestry Biosecurity, Institute of Applied Ecology Fujian Agriculture and Forestry University  Fuzhou China"},{"name":"Joint International Research Laboratory of Ecological Pest Control Ministry of Education  Fuzhou China"},{"name":"Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross\u2010Strait Crops Fujian Agriculture and Forestry University  Fuzhou China"},{"name":"Key Laboratory of Integrated Pest Management for Fujian\u2010Taiwan Crops Ministry of Agriculture  Fuzhou China"},{"name":"Key Laboratory of Green Pest Control Fujian Province University  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3250-5228","authenticated-orcid":false,"given":"Guang","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural and Forestry Biosecurity, Institute of Applied Ecology Fujian Agriculture and Forestry University  Fuzhou China"},{"name":"Joint International Research Laboratory of Ecological Pest Control Ministry of Education  Fuzhou China"},{"name":"Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross\u2010Strait Crops Fujian Agriculture and Forestry University  Fuzhou China"},{"name":"Key Laboratory of Integrated Pest Management for Fujian\u2010Taiwan Crops Ministry of Agriculture  Fuzhou China"},{"name":"Key Laboratory of Green Pest Control Fujian Province University  Fuzhou 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