{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T11:08:44Z","timestamp":1788865724483,"version":"build-2803163510"},"reference-count":59,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T00:00:00Z","timestamp":1779840000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T00:00:00Z","timestamp":1779840000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100017359","name":"Shandong Province Key Agricultural Project for Application Technology Innovation","doi-asserted-by":"publisher","award":["SDAIT\u201031\u201004"],"award-info":[{"award-number":["SDAIT\u201031\u201004"]}],"id":[{"id":"10.13039\/100017359","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32272530"],"award-info":[{"award-number":["32272530"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Molecular Biology"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Insect guts host a diverse and abundant array of microorganisms. These microbes improve host fitness by extensively involving in a range of crucial physiological processes, which have mainly been revealed by high\u2010throughput sequencing, particularly metagenomics. However, it is almost impossible to make an accurate and complete distinction between the genetic functions of microbial symbionts and insect hosts without host genome data. By comparing metagenomic data from gut germ\u2010free and nonaxenic larvae, we accurately identified the data belonging to the gut microbiome of the onion maggot\n                    <jats:italic>Delia antiqua<\/jats:italic>\n                    (Diptera: Anthomyiidae). Besides, a correlation between bacteria of the genus\n                    <jats:italic>Wohlfahrtiimonas<\/jats:italic>\n                    (Gammaproteobacteria: Pseudomonadaceae) and vitamin B6 metabolism was detected through collinearity analysis. Furthermore, in vitro tests confirmed that the gut bacterium\n                    <jats:italic>Wohlfahrtiimonas larvae<\/jats:italic>\n                    contributed to the growth of\n                    <jats:italic>D. antiqua<\/jats:italic>\n                    larvae via the independent synthesis of vitamin B6. This study provides a comprehensive view of the gut bacterial diversity in\n                    <jats:italic>D. antiqua<\/jats:italic>\n                    and reveals a functional profile that is strictly specific to the gut microbiota of this species. It has preliminarily revealed the functional differentiation between insect hosts and their symbiotic microorganisms. This study also offers a technical reference for the study of microbial symbiotic functions in other insect\u2010microbe symbioses without host genomic data.\n                  <\/jats:p>","DOI":"10.1111\/imb.70046","type":"journal-article","created":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T10:23:59Z","timestamp":1779877439000},"page":"489-503","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Host\u2010independent metagenomics reveal gut bacteria contribution to\n                    <i>Delia antiqua<\/i>\n                    growth by vitamin\n                    <scp>B6<\/scp>\n                    provision"],"prefix":"10.1111","volume":"35","author":[{"given":"Luyao","family":"Lin","sequence":"first","affiliation":[{"name":"Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences)  Ji'nan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guiyue","family":"Gao","sequence":"additional","affiliation":[{"name":"Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences)  Ji'nan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuoshuo","family":"Sun","sequence":"additional","affiliation":[{"name":"Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences)  Ji'nan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoqing","family":"Wu","sequence":"additional","affiliation":[{"name":"Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences)  Ji'nan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susu","family":"Fan","sequence":"additional","affiliation":[{"name":"Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences)  Ji'nan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hengliang","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Plant Protection, Henan Academy of Agricultural Sciences  Zhengzhou Henan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7603-2303","authenticated-orcid":false,"given":"Fangyuan","family":"Zhou","sequence":"additional","affiliation":[{"name":"Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences)  Ji'nan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5839-6197","authenticated-orcid":false,"given":"Xinjian","family":"Zhang","sequence":"additional","affiliation":[{"name":"Ecology Institute, Qilu University of Technology (Shandong Academy of Sciences)  Ji'nan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,27]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0022-2836(05)80360-2"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/mr.59.1.143-169.1995"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-45024-5"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1265"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00248-024-02431-x"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3791\/66259"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocontrol.2019.104156"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2014.04.005"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-ento-010814-020822"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/aesa\/78.2.182"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11626-014-9758-x"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1558-5646.1985.tb00420.x"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2011.02.005"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/gigascience\/giaf004"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2024.01.070"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41396-022-01237-4"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40168-024-01931-9"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/eea.12768"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/mst012"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1673\/031.013.9901"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2024.12.028"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibiod.2017.02.015"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/NMETH.1923"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/insects16060611"},{"key":"e_1_2_10_26_1","unstructured":"Lin L.Y. Gao G.Y. Sun S.S. Wu X.Q. Fan S.S. Wang H.L.et al. (2026a)16S rRNA sequences of 17 gut bacterial strains associated with Delia antiqua (accession nos. C_AA165265.1\u2013C_AA165281.1)[dataset]. GenBase China National Center for Bioinformation (CNCB). Available at:https:\/\/ngdc.cncb.ac.cn\/genbase\/browse\/0009329. Accessed 3 April 2026."},{"key":"e_1_2_10_27_1","unstructured":"Lin L.Y. Gao G.Y. Sun S.S. Wu X.Q. Fan S.S. Wang H.L.et al. (2026b)Host\u2010independent metagenomics reveal gut bacteria contribution to Delia antiqua growth by vitamin B6 provision[dataset]. Genome Sequence Archive (GSA) China National Center for Bioinformation (CNCB). Available at:https:\/\/ngdc.cncb.ac.cn\/gsa\/browse\/CRA040349. Accessed 31 March 2026."},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoenv.2019.01.109"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/jee\/toab002"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-025-56354-3"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadi.2023.166901"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01150-14"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules14010329"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2020.107550"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471250953.bi0301s42"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajp.22014"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2024.1343265"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/12.13.5529"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1093\/jisesa\/iead024"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1570-7458.1983.tb03232.x"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.genet.41.110306.130354"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.02225-22"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.10010825"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.00091"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0014-5793(01)02705-3"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2011.07.012"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-011-3800-7"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2003.08.057"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1111\/jipb.13505"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature06269"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-025-24795-x"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.24121651211"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40168-022-01290-3"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1093\/ismejo\/wrae031"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40643-023-00656-1"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.710800"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoenv.2023.115649"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2024.173866"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.5084"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.1128\/mSystems.00778-20"}],"container-title":["Insect Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/resjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/imb.70046","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/resjournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/imb.70046","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/resjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/imb.70046","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T10:25:44Z","timestamp":1788863144000},"score":1,"resource":{"primary":{"URL":"https:\/\/resjournals.onlinelibrary.wiley.com\/doi\/10.1111\/imb.70046"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,27]]},"references-count":59,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1111\/imb.70046"],"URL":"https:\/\/doi.org\/10.1111\/imb.70046","archive":["Portico"],"relation":{},"ISSN":["0962-1075","1365-2583"],"issn-type":[{"value":"0962-1075","type":"print"},{"value":"1365-2583","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,27]]},"assertion":[{"value":"2026-01-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-27","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}