{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T11:08:41Z","timestamp":1788865721550,"version":"build-2803163510"},"reference-count":125,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T00:00:00Z","timestamp":1780531200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T00:00:00Z","timestamp":1780531200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000038","name":"Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["RGPIN\u20102023\u201004126"],"award-info":[{"award-number":["RGPIN\u20102023\u201004126"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Molecular Biology"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    DEET and citronella oil are two of the most widely used mosquito repellents; however, their impact on chemosensory tissues is not fully understood. In their vapour phase, the repellency effect involves the olfactory system, with mosquitoes detecting and responding to diverse odorants through their antennae and mouthparts (maxillary palps and proboscis). In this study, we first performed repellency behavioural assays that indicated both DEET and citronella effectively impaired female\n                    <jats:italic>Aedes aegypti<\/jats:italic>\n                    host\u2010seeking capabilities (i.e. landing on a host and taking a blood meal). We next used RNA sequencing to assess global gene expression changes in\n                    <jats:italic>Ae. aegypti<\/jats:italic>\n                    antennae, mouthparts and headless bodies in response to volatile repellent exposure. The resultant transcriptional changes were considerably greater in the mosquito mouthparts than in the other tissues. The most notable responses in olfactory\u2010related genes to both repellents were the suppressed expression of the three subunits comprising the mosquito CO\n                    <jats:sub>2<\/jats:sub>\n                    receptor (Gr1, 2 and 3). Genes associated with mitochondrial function and metabolism were also suppressed. Exposure to DEET was further characterized by substantial inhibition of cytochrome P450 mRNA expression, the primary detoxification enzymes in insects. Overall, our study provides novel insights into the olfactory and physiological responses of\n                    <jats:italic>Ae. aegypti<\/jats:italic>\n                    chemosensory tissues in response to DEET and citronella exposure.\n                  <\/jats:p>","DOI":"10.1111\/imb.70049","type":"journal-article","created":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T06:11:47Z","timestamp":1780639907000},"page":"526-540","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Transcriptional responses of\n                    <scp>\n                      <i>Aedes aegypti<\/i>\n                    <\/scp>\n                    chemosensory tissues in response to volatile\n                    <scp>DEET<\/scp>\n                    and citronella oil"],"prefix":"10.1111","volume":"35","author":[{"given":"Ivan","family":"Drahun","sequence":"first","affiliation":[{"name":"Department of Biology Brandon University  Brandon Manitoba Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica M.","family":"Sparrow","sequence":"additional","affiliation":[{"name":"Department of Biology Brandon University  Brandon Manitoba Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5133-6135","authenticated-orcid":false,"given":"Cody W.","family":"Koloski","sequence":"additional","affiliation":[{"name":"Department of Biology Brandon University  Brandon Manitoba Canada"},{"name":"Department of Veterinary Microbiology Western College of Veterinary Medicine, University of Saskatchewan  Saskatoon Saskatchewan Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3479-5676","authenticated-orcid":false,"given":"Bryan J.","family":"Cassone","sequence":"additional","affiliation":[{"name":"Department of Biology Brandon University  Brandon Manitoba Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,4]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2019.09.007"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-020-03206-8"},{"key":"e_1_2_10_4_1","unstructured":"Andrews S.(2010)FastQC: a quality control tool for high throughput sequence data. Available at:http:\/\/www.bioinformatics.babraham.ac.uk\/projects\/fastqc"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0012138"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2915.2011.00949.x"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2014.02.007"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/1756-3305-2-17"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1103472108"},{"key":"e_1_2_10_10_1","unstructured":"Cassone B.J.(2026)Elucidating the molecular basis for DEET and citronella repellency in mosquitoes. NCBI Sequence Read Archive (SRA). Accession Numbers: PRJNA793033(SRX13656900\u2010 SRX13656932). Available at:https:\/\/www.ncbi.nlm.nih.gov\/sra"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10886-016-0770-3"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/1741-7007-7-47"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/19336934.2015.1079360"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12206"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.01736"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/jbt.22913"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2019.04.004"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02531-21"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0029147"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.2987\/17-6673.1"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep43618"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2583.2011.01120.x"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-128-11-199806010-00013"},{"key":"e_1_2_10_24_1","first-page":"43","volume-title":"Travel Medicine (Fourth Edition)","author":"Fradin M.S.","year":"2019"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa011699"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1603\/0022-2585-41.3.414"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku1117"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-022-05401-9"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/9780470514948.ch17"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.5592"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.5772\/intechopen.80283"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00049-009-0033-7"},{"key":"e_1_2_10_33_1","volume-title":"Pest Management Regulatory Agency","author":"Health Canada","year":"2002"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2915.2000.00238.x"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2022.07.024"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7322(99)00031-8"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2007-8-9-r183"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471250953.bi1311s27"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0048698"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-022-04185-z"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-820267-8.00032-7"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.3389\/fnmol.2018.00366"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ttbdis.2021.101827"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10493-020-00550-x"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ttbdis.2018.12.006"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3156.2011.02781.x"},{"key":"e_1_2_10_47_1","first-page":"209","article-title":"DEET\u2010based insect repellents: safety implications for children and pregnant and lactating women","volume":"169","author":"Koren G.","year":"2003","journal-title":"Canadian Medical Association Journal"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2020.03.074"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2010.08.016"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.3390\/insects14010049"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.20242"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1316059110"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2019.06.001"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-014-0550-8"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cois.2020.11.004"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2007.07.062"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecoenv.2020.111232"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2875-10-S1-S11"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0293018"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-018-0692-z"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.1093\/jmedent\/19.5.489"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.12.044"},{"key":"e_1_2_10_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2025.02.009"},{"key":"e_1_2_10_64_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2022.897174"},{"key":"e_1_2_10_65_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2021.12.017"},{"key":"e_1_2_10_66_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2021.668236"},{"key":"e_1_2_10_67_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2025.05.004"},{"key":"e_1_2_10_68_1","doi-asserted-by":"publisher","DOI":"10.1201\/9781420006650.ch14"},{"key":"e_1_2_10_69_1","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2875-9-127"},{"key":"e_1_2_10_70_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.conb.2009.07.015"},{"key":"e_1_2_10_71_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-022-05196-9"},{"key":"e_1_2_10_72_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-801694-7.00006-8"},{"key":"e_1_2_10_73_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10438"},{"key":"e_1_2_10_74_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/29.9.e45"},{"key":"e_1_2_10_75_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0807167105"},{"key":"e_1_2_10_76_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-24923-x"},{"key":"e_1_2_10_77_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.02.003"},{"key":"e_1_2_10_78_1","volume-title":"R: A language and environment for statistical computing","author":"R Core Team","year":"2021"},{"key":"e_1_2_10_79_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.conb.2015.06.014"},{"key":"e_1_2_10_80_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2019.09.053"},{"key":"e_1_2_10_81_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom11040509"},{"key":"e_1_2_10_82_1","volume-title":"RNAseq in the mosquito maxillary palp: a little antennal RNA goes a long way","author":"Rinker D.C.","year":"2015"},{"key":"e_1_2_10_83_1","volume-title":"RStudio: Integrated Development for R","author":"RStudio Team","year":"2019"},{"key":"e_1_2_10_84_1","doi-asserted-by":"publisher","DOI":"10.1093\/jmedent\/14.5.536"},{"key":"e_1_2_10_85_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0048554"},{"key":"e_1_2_10_86_1","doi-asserted-by":"publisher","DOI":"10.3389\/finsc.2021.765179"},{"key":"e_1_2_10_87_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-23987-y"},{"key":"e_1_2_10_88_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.73"},{"key":"e_1_2_10_89_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.354"},{"key":"e_1_2_10_90_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinsphys.2021.104263"},{"key":"e_1_2_10_91_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-19580-z"},{"key":"e_1_2_10_92_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2023.108699"},{"key":"e_1_2_10_93_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2021.103550"},{"key":"e_1_2_10_94_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0813264106"},{"key":"e_1_2_10_95_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002631"},{"key":"e_1_2_10_96_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2014.02.004"},{"key":"e_1_2_10_97_1","doi-asserted-by":"publisher","DOI":"10.3390\/insects12070593"},{"key":"e_1_2_10_98_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1001313107"},{"key":"e_1_2_10_99_1","doi-asserted-by":"publisher","DOI":"10.1139\/z63-030"},{"key":"e_1_2_10_100_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-323-89846-1.00002-4"},{"key":"e_1_2_10_101_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cois.2014.10.006"},{"key":"e_1_2_10_102_1","first-page":"309","article-title":"Sensory bases of attractancy: morphology of mosquito olfactory sensilla\u2010a review","volume":"10","author":"Sutcliffe J.F.","year":"1994","journal-title":"Journal of the American Mosquito Control Association"},{"key":"e_1_2_10_103_1","doi-asserted-by":"publisher","DOI":"10.1093\/chemse\/bjm040"},{"key":"e_1_2_10_104_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0805312105"},{"key":"e_1_2_10_105_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0017705"},{"key":"e_1_2_10_106_1","doi-asserted-by":"publisher","DOI":"10.37212\/jcnos.1080039"},{"key":"e_1_2_10_107_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2021.104938"},{"key":"e_1_2_10_108_1","volume-title":"Book Review: The biology of mosquitoes Vol. 2\u2010Sensory Reception and behaviour","author":"Takken W.","year":"2000"},{"key":"e_1_2_10_109_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.11.013"},{"key":"e_1_2_10_110_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00191.2022"},{"key":"e_1_2_10_111_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10081"},{"key":"e_1_2_10_112_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature08295"},{"key":"e_1_2_10_113_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2019.06.043"},{"key":"e_1_2_10_114_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2024.175422"},{"key":"e_1_2_10_115_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-020-03407-2"},{"key":"e_1_2_10_116_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature06861"},{"key":"e_1_2_10_117_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0160009"},{"key":"e_1_2_10_118_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-75782-3"},{"key":"e_1_2_10_119_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.51040"},{"key":"e_1_2_10_120_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1417244111"},{"key":"e_1_2_10_121_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00204-022-03267-5"},{"key":"e_1_2_10_122_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-13-134"},{"key":"e_1_2_10_123_1","doi-asserted-by":"publisher","DOI":"10.3390\/agriculture12010053"},{"key":"e_1_2_10_124_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox11112241"},{"key":"e_1_2_10_125_1","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/361021"},{"key":"e_1_2_10_126_1","volume-title":"Chemosensory transduction: the detection of odors, tastes, and other chemostimuli","author":"Zufall F.","year":"2016"}],"container-title":["Insect Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/resjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/imb.70049","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/resjournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/imb.70049","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/resjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/imb.70049","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T10:25:41Z","timestamp":1788863141000},"score":1,"resource":{"primary":{"URL":"https:\/\/resjournals.onlinelibrary.wiley.com\/doi\/10.1111\/imb.70049"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,4]]},"references-count":125,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1111\/imb.70049"],"URL":"https:\/\/doi.org\/10.1111\/imb.70049","archive":["Portico"],"relation":{},"ISSN":["0962-1075","1365-2583"],"issn-type":[{"value":"0962-1075","type":"print"},{"value":"1365-2583","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,4]]},"assertion":[{"value":"2025-12-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}