{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T11:08:44Z","timestamp":1788865724430,"version":"build-2803163510"},"reference-count":106,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T00:00:00Z","timestamp":1781913600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T00:00:00Z","timestamp":1781913600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100013704","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda, Paraguay","doi-asserted-by":"publisher","award":["CVU421099"],"award-info":[{"award-number":["CVU421099"]}],"id":[{"id":"10.13039\/501100013704","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Molecular Biology"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Malaria\u2010causing\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    parasites are transmitted by some\n                    <jats:italic>Anopheles<\/jats:italic>\n                    mosquito species. The genetic and immunity basis for vector competence, the mosquitoes' intrinsic ability to transmit pathogens, has been extensively studied in major vectors such as\n                    <jats:italic>Anopheles gambiae<\/jats:italic>\n                    and\n                    <jats:italic>Anopeles stephensi<\/jats:italic>\n                    . In\n                    <jats:italic>An<\/jats:italic>\n                    .\n                    <jats:italic>gambiae<\/jats:italic>\n                    , Lola\u2010type motifs were identified in immunity\u2010related\n                    <jats:italic>cis<\/jats:italic>\n                    \u2010regulatory elements; the Lola transcription factor is known to be involved in\n                    <jats:italic>Drosophila<\/jats:italic>\n                    development. Here, the potential role of\n                    <jats:italic>lola<\/jats:italic>\n                    in mosquito antimicrobial defence was investigated in\n                    <jats:italic>Anopheles albimanus<\/jats:italic>\n                    , a malaria vector in the Americas. The in vivo attenuation of\n                    <jats:italic>lola<\/jats:italic>\n                    was found to compromise mosquito survival to microbial infection. It was also found that the induction of antimicrobial defence genes requires\n                    <jats:italic>lola<\/jats:italic>\n                    upregulation. Genes for antimicrobial peptides, C\u2010lectins, pattern recognition receptors, Clip\u2010domain serine proteases and ML\u2010domain proteins showed\n                    <jats:italic>lola<\/jats:italic>\n                    upregulation dependency. Enzymes involved in the generation and detoxification of reactive oxygen species, as well as components of the JNK immune signalling pathways, are also dependent on\n                    <jats:italic>lola<\/jats:italic>\n                    upregulation. These findings suggest that\n                    <jats:italic>lola<\/jats:italic>\n                    regulates the expression of immunity and detoxification genes and that there is a functional link between JNK signalling and\n                    <jats:italic>lola<\/jats:italic>\n                    expression in the mosquito midgut. Dysregulation of this pathway may contribute to increased pathogen invasion and malaria transmission.\n                  <\/jats:p>","DOI":"10.1111\/imb.70051","type":"journal-article","created":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T11:14:13Z","timestamp":1781954053000},"page":"576-593","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Malaria mosquito antimicrobial defence requires immunity and detoxification gene regulation by Lola"],"prefix":"10.1111","volume":"35","author":[{"given":"Heidi","family":"Espadas\u2010\u00c1lvarez","sequence":"first","affiliation":[{"name":"Laboratorio de Gen\u00e9tica Molecular, Centro de Investigaciones Sobre Enfermedades Infecciosas Instituto Nacional de Salud P\u00fablica  Cuernavaca Morelos Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1490-2905","authenticated-orcid":false,"given":"Ver\u00f3nica","family":"Valverde\u2010Gardu\u00f1o","sequence":"additional","affiliation":[{"name":"Laboratorio de Gen\u00e9tica Molecular, Centro de Investigaciones Sobre Enfermedades Infecciosas Instituto Nacional de Salud P\u00fablica  Cuernavaca Morelos Mexico"},{"name":"Facultad de Ciencias Universidad Nacional Aut\u00f3noma de M\u00e9xico, Ciudad Universitaria  Ciudad de M\u00e9xico Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,20]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2004.03.019"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001317"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.redox.2019.101340"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/18.4.959"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.81116"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ydbio.2007.01.029"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu170"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81226-X"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.13906"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-9-425"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-60327-295-7_5"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1159\/000353602"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkad965"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1534\/g3.120.401654"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.129.6.1317"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2025.116921"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.202302684RR"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.264792"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts635"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.20.091301.131133"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.018036"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/30.1.207"},{"key":"e_1_2_8_24_1","first-page":"6675","article-title":"DNA binding specificity of different STAT proteins. Comparison of in vitro specificity with natural target sites","volume":"276","author":"Ehret G.B.","year":"2001","journal-title":"American Society of Biological Chemists"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni1335"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003622"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.2174\/1566524023362375"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/1749-8104-6-37"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btz931"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1159\/000452797"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.120.6.1385"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku1117"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn1105"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2009.04.003"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.47542"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.en.28.010183.001305"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.76132"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41423-018-0007-1"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0011168"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2012.07.007"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2020.572340"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2005.03.014"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.1.27"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cois.2015.09.003"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.01863"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2014.11.005"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-016-1810-0"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7600807"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.3390\/insects9030095"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbc.2021.101435"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-025-06658-6"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btt656"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2217"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2013.30"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M408597200"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.12.4.557"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1261\/rna.2116303"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0300-483X(00)00306-1"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2583.2006.00635.x"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2005.02.009"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1186\/1756-3305-6-146"},{"key":"e_1_2_8_62_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2583.2003.00387.x"},{"key":"e_1_2_8_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2006.01.004"},{"key":"e_1_2_8_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2011.04.001"},{"key":"e_1_2_8_65_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-ento-070621-061328"},{"key":"e_1_2_8_66_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1258522"},{"key":"e_1_2_8_67_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.451427"},{"key":"e_1_2_8_68_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox12081616"},{"key":"e_1_2_8_69_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(03)00554-7"},{"key":"e_1_2_8_70_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1168104"},{"key":"e_1_2_8_71_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0186435"},{"key":"e_1_2_8_72_1","article-title":"A protocol for collecting and staining hemocytes from the yellow fever mosquito Aedes aegypti","volume":"51","author":"Qayum A.A.","year":"2011","journal-title":"Journal of Visualized Experiments"},{"key":"e_1_2_8_73_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/cdg355"},{"key":"e_1_2_8_74_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4889(00)00020-3"},{"key":"e_1_2_8_75_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81274-X"},{"key":"e_1_2_8_76_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1423586112"},{"key":"e_1_2_8_77_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST0390559"},{"key":"e_1_2_8_78_1","doi-asserted-by":"publisher","DOI":"10.1242\/jeb.221622"},{"key":"e_1_2_8_79_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.73"},{"key":"e_1_2_8_80_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.00609"},{"key":"e_1_2_8_81_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ibmb.2012.04.002"},{"key":"e_1_2_8_82_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.189.11.1777"},{"key":"e_1_2_8_83_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M500711200"},{"key":"e_1_2_8_84_1","doi-asserted-by":"publisher","DOI":"10.1111\/imb.12383"},{"key":"e_1_2_8_85_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0167283"},{"key":"e_1_2_8_86_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M304802200"},{"key":"e_1_2_8_87_1","doi-asserted-by":"publisher","DOI":"10.1186\/1756-3305-3-72"},{"key":"e_1_2_8_88_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002965"},{"key":"e_1_2_8_89_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1007418"},{"key":"e_1_2_8_90_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2020.06.017"},{"key":"e_1_2_8_91_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0294236"},{"key":"e_1_2_8_92_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0605988103"},{"key":"e_1_2_8_93_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.22-0417"},{"key":"e_1_2_8_94_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2020.103964"},{"key":"e_1_2_8_95_1","unstructured":"Valverde\u2010Gardu\u00f1o V.&Espadas\u2010\u00c1lvarez H.G.(2026)Anopheles albimanuslola knockdown immune response differential transcriptome: effect ofAnopheles albimanuslola knockdown on the gene expression profile of the midgut in mosquitoes treated with an immune challenge. Gene Expression Omnibus; Accession GSE 317204. Available from:https:\/\/www.ncbi.nlm.nih.gov\/geo\/query\/acc.cgi?acc=GSE317204"},{"key":"e_1_2_8_96_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13071-021-05091-9"},{"key":"e_1_2_8_97_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1534-5807(03)00323-X"},{"key":"e_1_2_8_98_1","first-page":"477","article-title":"Research advances on immune mechanism against pathogens in mosquitoes","volume":"24","author":"Wang Y.H.","year":"2013","journal-title":"Chinese Journal of Vector Biology and Control"},{"key":"e_1_2_8_99_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.04.049"},{"key":"e_1_2_8_100_1","volume-title":"World malaria report 2024","author":"WHO","year":"2024"},{"key":"e_1_2_8_101_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mod.2009.06.1082"},{"key":"e_1_2_8_102_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms242115808"},{"key":"e_1_2_8_103_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsob.220111"},{"key":"e_1_2_8_104_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2017.00022"},{"key":"e_1_2_8_105_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2021.110003"},{"key":"e_1_2_8_106_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M213269200"},{"key":"e_1_2_8_107_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-022-05195-9"}],"container-title":["Insect Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/resjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/imb.70051","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/resjournals.onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/imb.70051","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/resjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/imb.70051","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T10:25:33Z","timestamp":1788863133000},"score":1,"resource":{"primary":{"URL":"https:\/\/resjournals.onlinelibrary.wiley.com\/doi\/10.1111\/imb.70051"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,20]]},"references-count":106,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,10]]}},"alternative-id":["10.1111\/imb.70051"],"URL":"https:\/\/doi.org\/10.1111\/imb.70051","archive":["Portico"],"relation":{},"ISSN":["0962-1075","1365-2583"],"issn-type":[{"value":"0962-1075","type":"print"},{"value":"1365-2583","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,20]]},"assertion":[{"value":"2026-01-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-20","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}