{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T08:22:48Z","timestamp":1789114968676,"version":"build-2803163510"},"reference-count":52,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T00:00:00Z","timestamp":1789084800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T00:00:00Z","timestamp":1789084800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFE0104800"],"award-info":[{"award-number":["2023YFE0104800"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32460673"],"award-info":[{"award-number":["32460673"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32670681"],"award-info":[{"award-number":["32670681"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Molecular Biology"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Western flower thrips (WFT),\n                    <jats:italic>Frankliniella occidentalis<\/jats:italic>\n                    (Pergande) (Thysanoptera: Thripidae), is a globally invasive polyphagous pest that relies on olfaction to sense host plants. Odorant\u2010binding proteins (OBPs) in insect sensillar lymph mediate interactions with plant volatiles. However, the physicochemical mechanisms by which OBPs in\n                    <jats:italic>F. occidentalis<\/jats:italic>\n                    bind to host\u2010plant volatiles, the key amino acid residues involved in these interactions and the structural characteristics of the preferred ligands remain unclear. Here, we cloned a novel OBP,\n                    <jats:italic>Focc<\/jats:italic>\n                    OBP8, which belongs to the C\u2010minus OBP subfamily based on sequence alignment and phylogenetic analyses. Competitive fluorescence binding assays using purified recombinant\n                    <jats:italic>Focc<\/jats:italic>\n                    OBP8 revealed broad binding interactions towards host\u2010plant volatiles. Notably, 4\u2010ethylacetophenone and ethyl isonicotinate showed the strongest binding affinity with dissociation constants (\n                    <jats:italic>K<\/jats:italic>\n                    <jats:sub>D<\/jats:sub>\n                    ) of 2.00 and 2.46\u2009\u03bcmol\/L, respectively. Thermodynamic analyses indicated the binding processes were dynamic quenching processes and mainly driven by hydrophobic interactions, spontaneously. Molecular docking and the corresponding residue\u2010interaction energy heatmap analyses predicted that the conserved Gln4 residue contributed substantially to the ligand\u2010protein interactions. Molecule clustering and structural analyses predicted that the ligands containing aromatic moieties \u03c0\u2013\u03c0 conjugated with the carbonyl group or carbon\u2013carbon double bond preferentially bound to\n                    <jats:italic>Focc<\/jats:italic>\n                    OBP8. This study elucidates the broad binding profiles of\n                    <jats:italic>Focc<\/jats:italic>\n                    OBP8 with ligands and their physicochemical basis, providing insights into the olfactory\u2010driven polyphagia of WFT and theoretical foundations for developing odour\u2010based attractants or repellents targeting this invasive pest.\n                  <\/jats:p>","DOI":"10.1111\/imb.70063","type":"journal-article","created":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T07:26:18Z","timestamp":1789111578000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Binding characteristics and physicochemical basis of\n                    <scp>C<\/scp>\n                    \u2010minus odorant\u2010binding protein 8 in western flower thrips,\n                    <i>Frankliniella occidentalis<\/i>\n                    , with various host vegetable volatiles"],"prefix":"10.1111","author":[{"given":"Hong\u2010Qi","family":"Zhang","sequence":"first","affiliation":[{"name":"College of Life Sciences, China Jiliang University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia\u2010Qi","family":"Huang","sequence":"additional","affiliation":[{"name":"College of Life Sciences, China Jiliang University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng\u2010Yang","family":"Li","sequence":"additional","affiliation":[{"name":"College of Life Sciences, China Jiliang University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu\u2010Sen","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Life Sciences, China Jiliang University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin\u2010Xuan","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Life Sciences, China Jiliang University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5398-278X","authenticated-orcid":false,"given":"Zhi\u2010Ke","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Plant Protection, Ningxia Academy of Agricultural and Forestry Sciences  Yinchuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7094-2472","authenticated-orcid":false,"given":"Hong\u2010Liang","family":"Li","sequence":"additional","affiliation":[{"name":"College of Life Sciences, China Jiliang University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,11]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1118446"},{"issue":"6","key":"e_1_2_9_3_1","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1016\/j.disamonth.2009.03.003","article-title":"Cinnamon (Cinnamomum species)","volume":"55","author":"Barceloux 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Li H.\u2010L.&Zhang H.\u2010Q.(2025)Odorant\u2010binding protein 8 [Frankliniella occidentalis] (version 1) [dataset]. GenBank. accession XRS46039.1; Available from:https:\/\/www.ncbi.nlm.nih.gov\/protein\/XRS46039.1\/"},{"key":"e_1_2_9_45_1","unstructured":"Zhang Z.\u2010K. Li H.\u2010L.&Zhang H.\u2010Q.(2026)Data and code from: binding characteristics and physicochemical basis of C\u2010minus odorant\u2010binding protein 8 in western flower thrips Frankliniella occidentaliswith various host vegetable volatiles (Version 5) [Dataset]. Dryad. 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