{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T03:55:03Z","timestamp":1790999703460,"version":"4.1.0"},"reference-count":48,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T00:00:00Z","timestamp":1790899200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T00:00:00Z","timestamp":1790899200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001412","name":"Council of Scientific and Industrial Research, India","doi-asserted-by":"publisher","award":["YSA000826"],"award-info":[{"award-number":["YSA000826"]}],"id":[{"id":"10.13039\/501100001412","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["resjournals.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Insect Molecular Biology"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Trehalose is a principal circulating sugar in insects, maintaining a critical balance between energy supply and storage through systemic insulin signalling. Among different tissues, insect muscle functions as a metabolic hub, coordinating trehalose utilization and glucose uptake to sustain energy homeostasis. In\n                    <jats:italic>Helicoverpa armigera<\/jats:italic>\n                    , we examined whether\n                    <jats:italic>paramyosin<\/jats:italic>\n                    (key muscle gene) silencing influence carbohydrate metabolism.\n                    <jats:italic>HaPrm<\/jats:italic>\n                    silencing led to muscle defects and delayed pupation, indicating a crucial role of\n                    <jats:italic>HaPrm<\/jats:italic>\n                    in development. Transcriptomics revealed dysregulation of genes involved in carbohydrate metabolism and insulin\u2010mTOR signalling. Notably, expression and enzymatic activities of\n                    <jats:italic>HaTPS<\/jats:italic>\n                    \/\n                    <jats:italic>TPP<\/jats:italic>\n                    and\n                    <jats:italic>HaTreh<\/jats:italic>\n                    (\n                    <jats:italic>s<\/jats:italic>\n                    ) were reduced in\n                    <jats:italic>HaPrm<\/jats:italic>\n                    \u2010silenced insects, accompanied by their altered metabolite intermediate levels. Given that trehalose metabolism fuels both glycolysis and glycogen synthesis,\n                    <jats:italic>HaPrm<\/jats:italic>\n                    silencing also altered the expression of glycolytic, glycogen metabolism and energy\u2010sensing genes. Metabolomics further demonstrated differential accumulation of intermediates from glycolysis, glycogen recycling, the TCA cycle and amino acid metabolism. Moreover, insulin\u2010mTOR signalling genes and the glucose\u2010sensing transcription factor were significantly affected, highlighting the sensitivity of nutrient\u2010sensing mechanisms to\n                    <jats:italic>HaPrm<\/jats:italic>\n                    silencing. Taken together, these results suggest that\n                    <jats:italic>HaPrm<\/jats:italic>\n                    disruption affects muscle integrity and is linked to changes in insulin\u2010mTOR signalling and trehalose metabolism. These data highlight the close relationship between muscle architecture and energy homeostasis in\n                    <jats:italic>H.\u2009armigera<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1111\/imb.70067","type":"journal-article","created":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T03:28:06Z","timestamp":1790998086000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Disruption of paramyosin compromises muscle integrity and alters systemic trehalose metabolism in\n                    <i>Helicoverpa armigera<\/i>"],"prefix":"10.1111","author":[{"given":"Sharada D.","family":"Mohite","sequence":"first","affiliation":[{"name":"Biochemical Sciences Division CSIR\u2010National Chemical Laboratory  Pune India"},{"name":"Academy of Scientific and Innovative Research (AcSIR)  Ghaziabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8016-1178","authenticated-orcid":false,"given":"Rakesh S.","family":"Joshi","sequence":"additional","affiliation":[{"name":"Biochemical Sciences Division CSIR\u2010National Chemical Laboratory  Pune India"},{"name":"Academy of Scientific and Innovative Research (AcSIR)  Ghaziabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,10,2]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"crossref","first-page":"638","DOI":"10.1016\/j.ijbiomac.2018.01.221","article-title":"Functional characterization of Helicoverpa armigera trehalase and investigation of physiological effects caused due to its inhibition by validamycin a formulation","volume":"112","author":"Adhav A.S.","year":"2018","journal-title":"International Journal of Biological 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