{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T13:40:59Z","timestamp":1791294059921,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,11,17]],"date-time":"2017-11-17T00:00:00Z","timestamp":1510876800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100013415","name":"R\u00e9gion Midi-Pyr\u00e9n\u00e9es","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100013415","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Universit\u00e9 Paul Sabatier"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Immunol Cell Biol"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We analyzed the impact of age, sex, and <jats:styled-content style=\"fixed-case\">CMV<\/jats:styled-content> on blood monocyte and dendritic cell (<jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>) subpopulations in 256 healthy individuals aged from 19 to 96\u00a0years. Flow cytometry was performed on whole blood within the 4\u00a0h following blood drawing. Myeloid (<jats:styled-content style=\"fixed-case\">mDC<\/jats:styled-content>) and plasmacytoid <jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">pDC<\/jats:styled-content>), classical, intermediate, and nonclassical monocytes were enumerated by means of TruCount tubes (<jats:styled-content style=\"fixed-case\">BD<\/jats:styled-content> Biosciences). We provided reference values for <jats:styled-content style=\"fixed-case\">mDC<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> pDC<\/jats:styled-content> and the three monocyte subpopulations. The numbers of classical, intermediate, and nonclassical monocytes slightly increased with age while the numbers of <jats:styled-content style=\"fixed-case\">mDC<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">pDC<\/jats:styled-content> did not vary significantly. The level of expression of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>64 and <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>163 on monocytes significantly increased with age while <jats:styled-content style=\"fixed-case\">HLA<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">DR<\/jats:styled-content> expression did not vary significantly. More precisely, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>163 expression level on intermediate monocyte slightly increased with age in women only (Spearman <jats:italic>P<\/jats:italic>\u00a0=\u00a00.019) while <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>64 expression increased on monocytes in <jats:styled-content style=\"fixed-case\">CMV<\/jats:styled-content>\u2010positive individuals only. We observed that sex had almost no impact on the numbers of monocytes and <jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content> and on their expression level of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>64 and <jats:styled-content style=\"fixed-case\">HLA<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">DR<\/jats:styled-content>. We observed a significant decrease in the numbers of <jats:styled-content style=\"fixed-case\">pDC<\/jats:styled-content> with age in <jats:styled-content style=\"fixed-case\">CMV<\/jats:styled-content>\u2010positive individuals, but not in <jats:styled-content style=\"fixed-case\">CMV<\/jats:styled-content> negative individuals. This suggests that the lifelong subclinical infection by <jats:styled-content style=\"fixed-case\">CMV<\/jats:styled-content> could influence the number of circulating <jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content> of lymphoid origin. In contrast, <jats:styled-content style=\"fixed-case\">CMV<\/jats:styled-content> serostatus had no significant impact on absolute numbers of <jats:styled-content style=\"fixed-case\">mDC<\/jats:styled-content> and monocytes.<\/jats:p>","DOI":"10.1111\/imcb.1004","type":"journal-article","created":{"date-parts":[[2018,1,12]],"date-time":"2018-01-12T11:20:08Z","timestamp":1515756008000},"page":"114-120","source":"Crossref","is-referenced-by-count":30,"title":["Distinct effect of age, sex, and <scp>CMV<\/scp> seropositivity on dendritic cells and monocytes in human blood"],"prefix":"10.1111","volume":"96","author":[{"given":"B\u00e9n\u00e9dicte","family":"Puissant\u2010Lubrano","sequence":"first","affiliation":[{"name":"Laboratoire d'Immunog\u00e9n\u00e9tique Mol\u00e9culaire, EA 3034 Universit\u00e9 Paul Sabatier Toulouse 3 France"},{"name":"Laboratoire d'Immunologie CHU de Toulouse Toulouse France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pol Andr\u00e9","family":"Apoil","sequence":"additional","affiliation":[{"name":"Laboratoire d'Immunog\u00e9n\u00e9tique Mol\u00e9culaire, EA 3034 Universit\u00e9 Paul Sabatier Toulouse 3 France"},{"name":"Laboratoire d'Immunologie CHU de Toulouse Toulouse France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin","family":"Guedj","sequence":"additional","affiliation":[{"name":"Laboratoire d'Immunog\u00e9n\u00e9tique Mol\u00e9culaire, EA 3034 Universit\u00e9 Paul Sabatier Toulouse 3 France"},{"name":"Laboratoire d'Immunologie CHU de Toulouse Toulouse France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas","family":"Congy\u2010Jolivet","sequence":"additional","affiliation":[{"name":"Laboratoire d'Immunog\u00e9n\u00e9tique Mol\u00e9culaire, EA 3034 Universit\u00e9 Paul Sabatier Toulouse 3 France"},{"name":"Laboratoire d'Immunologie CHU de Toulouse Toulouse 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