{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T21:23:47Z","timestamp":1790803427107,"version":"4.1.0"},"reference-count":48,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2018,4,16]],"date-time":"2018-04-16T00:00:00Z","timestamp":1523836800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001170","name":"Cancer Council Western Australia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001170","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001797","name":"Curtin University of Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001797","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunol Cell Biol"],"published-print":{"date-parts":[[2018,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The average age of the human population is rising, leading to an increasing burden of age\u2010related diseases, including increased susceptibility to infection. However, immune function can decrease with age which could impact on processes that require a functional immune system. Aging is also characterized by chronic low\u2010grade inflammation which could further impact immune cell function. While changes to neutrophils in blood during aging have been described, little is known in aging lymphoid organs. This study used female C57BL\/6J mice comparing bone marrow (BM), spleen and lymph nodes from young mice aged 2\u20133 months (equivalent to 18 human years) with healthy elderly mice aged 22\u201324 months (equivalent to 60\u201370 human years). Neutrophil proportions increased in BM and secondary lymphoid organs of elderly mice relative to their younger counterparts and presented an atypical phenotype. Interestingly, neutrophils from elderly spleen and lymph nodes were long lived (with decreased apoptosis via Annexin V staining and increased proportion of BrdU\n                    <jats:sup>neg<\/jats:sup>\n                    mature cells) with splenic neutrophils also demonstrating a hypersegmented morphology. Furthermore, splenic neutrophils of elderly mice expressed a mixed phenotype with increased expression of activation markers, CD11b and ICAM\u20101, increased proinflammatory TNF\u03b1, yet increased anti\u2010inflammatory transforming growth factor\u2010beta. Elderly splenic architecture was compromised, as the marginal zone (required for clearing infections) was contracted. Moreover, neutrophils from elderly but not young mice accumulated in lymph node and splenic T\u2010 and B\u2010cell zones. Overall, the expansion of functionally compromised neutrophils could contribute to increased susceptibility to infection observed in the elderly.\n                  <\/jats:p>","DOI":"10.1111\/imcb.12046","type":"journal-article","created":{"date-parts":[[2018,3,30]],"date-time":"2018-03-30T20:06:59Z","timestamp":1522440419000},"page":"831-840","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":51,"title":["Aged neutrophils accumulate in lymphoid tissues from healthy elderly mice and infiltrate T\u2010 and B\u2010cell zones"],"prefix":"10.1111","volume":"96","author":[{"given":"Federica","family":"Tomay","sequence":"first","affiliation":[{"name":"School of Pharmacy and Biomedical Sciences Curtin Health Innovation Research Institute Faculty of Health Sciences Curtin University Perth WA 6012 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