{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:48:25Z","timestamp":1791334105085,"version":"4.3.1"},"reference-count":30,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,12,13]],"date-time":"2018-12-13T00:00:00Z","timestamp":1544659200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Groupe Francophone de la Recherche sur la Scl\u00e9rodermie"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunol Cell Biol"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A defect in the apoptotic cell clearance (efferocytosis) by phagocytic cells may participate in autoimmunity and chronic inflammation. The mechanisms leading to the emergence of autoimmunity in systemic sclerosis (<jats:styled-content style=\"fixed-case\">SS<\/jats:styled-content>c) are still to be determined. In this study, the efferocytosis capacities of blood monocyte\u2010derived macrophages (<jats:styled-content style=\"fixed-case\">MDM<\/jats:styled-content>) from patients with <jats:styled-content style=\"fixed-case\">SS<\/jats:styled-content>c were evaluated. Blood monocytes obtained from patients with <jats:styled-content style=\"fixed-case\">SS<\/jats:styled-content>c and healthy donors (<jats:styled-content style=\"fixed-case\">HD<\/jats:styled-content>) were differentiated <jats:italic>in vitro<\/jats:italic> into macrophages. The capacities of <jats:styled-content style=\"fixed-case\">MDM<\/jats:styled-content> to engulf CFSE+ apoptotic Jurkat human T lymphocytes were compared between <jats:styled-content style=\"fixed-case\">SS<\/jats:styled-content>c <jats:styled-content style=\"fixed-case\">MDM<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">HD<\/jats:styled-content> using flow cytometry. The expression of classical engulfing receptors in <jats:styled-content style=\"fixed-case\">SS<\/jats:styled-content>c <jats:styled-content style=\"fixed-case\">MDM<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">HD MDM<\/jats:styled-content> was also evaluated and their involvement in the modulation of efferocytosis was confirmed using a si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> approach. The mean phagocytic index (<jats:styled-content style=\"fixed-case\">PI<\/jats:styled-content>) reflecting efferocytosis capacities of <jats:styled-content style=\"fixed-case\">SS<\/jats:styled-content>c <jats:styled-content style=\"fixed-case\">MDM<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">PI<\/jats:styled-content> = 19.3 \u00b1 3.0; <jats:italic>n<\/jats:italic> = 21) was significantly decreased in comparison with the <jats:styled-content style=\"fixed-case\">PI<\/jats:styled-content> of <jats:styled-content style=\"fixed-case\">HD MDM<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">PI<\/jats:styled-content> = 35.9 \u00b1 3.0; <jats:italic>n<\/jats:italic> = 31; <jats:italic>P<\/jats:italic> &lt; 0.001). In comparison with <jats:styled-content style=\"fixed-case\">HD<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> SS<\/jats:styled-content>c <jats:styled-content style=\"fixed-case\">MDM<\/jats:styled-content> exhibited a downregulated expression of scavenger receptor (<jats:styled-content style=\"fixed-case\">SR)<\/jats:styled-content>\u2010B1, <jats:styled-content style=\"fixed-case\">SR<\/jats:styled-content>\u2010A1 and integrin \u03b25 (ITG\u03b25). In <jats:styled-content style=\"fixed-case\">HD MDM<\/jats:styled-content>, the extinction of these receptors was followed by a reduction of efferocytosis only for the repression of ITG\u03b25, suggesting a possible selective role of this integrin in the impaired efferocytosis observed in <jats:styled-content style=\"fixed-case\">SS<\/jats:styled-content>c. As efferocytosis may be at the crossroads of inflammation, autoimmunity and fibrosis, in showing impaired efferocytosis capacities of blood <jats:styled-content style=\"fixed-case\">MDM<\/jats:styled-content> in <jats:styled-content style=\"fixed-case\">SS<\/jats:styled-content>c, our study offers new pathogenesis considerations for the involvement of macrophages in the autoimmune processes driving this disorder.<\/jats:p>","DOI":"10.1111\/imcb.12217","type":"journal-article","created":{"date-parts":[[2018,11,14]],"date-time":"2018-11-14T07:15:34Z","timestamp":1542179734000},"page":"340-347","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["Efferocytosis capacities of blood monocyte\u2010derived macrophages in systemic sclerosis"],"prefix":"10.1111","volume":"97","author":[{"given":"Alice","family":"Ballerie","sequence":"first","affiliation":[{"name":"Univ Rennes CHU Rennes INSERM EHESP IRSET (Institut de recherche 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IRSET (Institut de recherche en sant\u00e9, environnement et travail) \u2013 UMR_S 1085 35000 Rennes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudie","family":"Morzadec","sequence":"additional","affiliation":[{"name":"Univ Rennes CHU Rennes INSERM EHESP IRSET (Institut de recherche en sant\u00e9, environnement et travail) \u2013 UMR_S 1085 35000 Rennes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire","family":"Cazalets","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Rennes University Hospital 35000 Rennes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phane","family":"Jouneau","sequence":"additional","affiliation":[{"name":"Univ Rennes CHU Rennes INSERM EHESP IRSET (Institut de recherche en sant\u00e9, environnement et travail) \u2013 UMR_S 1085 35000 Rennes France"},{"name":"Department of Respiratory Diseases Rennes University Hospital 35000 Rennes 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