{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T19:03:08Z","timestamp":1760122988570,"version":"3.40.5"},"reference-count":70,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2020,8,4]],"date-time":"2020-08-04T00:00:00Z","timestamp":1596499200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100006449","name":"Fundaci\u00f3n Alberto J. Roemmers","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006449","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006668","name":"Fondo para la Investigaci\u00f3n Cient\u00edfica y Tecnol\u00f3gica","doi-asserted-by":"publisher","award":["PICT 2016\u20100356","PICT 2016\u20101945","PICT 2017\u20101393"],"award-info":[{"award-number":["PICT 2016\u20100356","PICT 2016\u20101945","PICT 2017\u20101393"]}],"id":[{"id":"10.13039\/501100006668","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunol Cell Biol"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Brucellosis is a contagious disease caused by bacteria of the genus <jats:italic>Brucella<\/jats:italic>. Platelets (PLTs) have been widely involved in the modulation of the immune response. We have previously reported the modulation of <jats:italic>Brucella abortus<\/jats:italic>\u2013mediated infection of monocytes. As a result, PLTs cooperate with monocytes and increase their inflammatory capacity, promoting the resolution of the infection. Extending these results, in this study we demonstrate that patients with brucellosis present slightly elevated levels of complexes between PLTs and both monocytes and neutrophils. We then assessed whether PLTs were capable of modulating functional aspects of neutrophils. The presence of PLTs throughout neutrophil infection increased the production of interleukin\u20108, CD11b surface expression and reactive oxygen species formation, whereas it decreased the expression of CD62L, indicating an activated status of these cells. We next analyzed whether this modulation was mediated by released factors. To discriminate between these options, neutrophils were treated with supernatants collected from <jats:italic>B. abortus<\/jats:italic>\u2013infected PLTs. Our results show that CD11b expression was induced by soluble factors of PLTs but direct contact between cell populations was needed to enhance the respiratory burst. Additionally, <jats:italic>B. abortus<\/jats:italic>\u2013infected PLTs recruit polymorphonuclear (PMN) cells to the site of infection. Finally, the presence of PLTs did not modify the initial invasion of PMN cells by <jats:italic>B. abortus<\/jats:italic> but improved the control of the infection at extended times. Altogether, our results demonstrate that PLTs interact with neutrophils and promote a proinflammatory phenotype which could also contribute to the resolution of the infection.<\/jats:p>","DOI":"10.1111\/imcb.12373","type":"journal-article","created":{"date-parts":[[2020,7,5]],"date-time":"2020-07-05T16:04:36Z","timestamp":1593965076000},"page":"743-756","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["<i>Brucella abortus<\/i>\u2013infected platelets modulate the activation of neutrophils"],"prefix":"10.1111","volume":"98","author":[{"given":"Aldana","family":"Trotta","sequence":"first","affiliation":[{"name":"Instituto de Medicina Experimental (CONICET\u2010Academia Nacional de Medicina) Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M Ayel\u00e9n","family":"Milillo","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Experimental (CONICET\u2010Academia Nacional de Medicina) Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agustina","family":"Serafino","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Experimental (CONICET\u2010Academia Nacional de Medicina) Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis A","family":"Castillo","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Experimental (CONICET\u2010Academia Nacional de Medicina) Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Federico","family":"Birnberg Weiss","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Experimental (CONICET\u2010Academia Nacional de Medicina) Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2077-8509","authenticated-orcid":false,"given":"M Victoria","family":"Delpino","sequence":"additional","affiliation":[{"name":"Instituto de Inmunolog\u00eda, Gen\u00e9tica y Metabolismo (INIGEM) CONICET Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guillermo H","family":"Giambartolomei","sequence":"additional","affiliation":[{"name":"Instituto de Inmunolog\u00eda, Gen\u00e9tica y Metabolismo (INIGEM) CONICET Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriela C","family":"Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Experimental (CONICET\u2010Academia Nacional de Medicina) Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paula","family":"Barrionuevo","sequence":"additional","affiliation":[{"name":"Instituto de Medicina Experimental (CONICET\u2010Academia Nacional de Medicina) Buenos Aires Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,8,4]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/5.5.821"},{"volume-title":"Enfermedades infecciosas: Brucelosis. Vol. 12, Gu\u00eda para el equipo de salud","year":"2013","author":"Moral M","key":"e_1_2_8_3_1"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.2174\/1574891X11308010005"},{"key":"e_1_2_8_5_1","first-page":"290","article-title":"Thrombocytopenia in brucellosis: case report and literature review","volume":"97","author":"Sevinc A","year":"2005","journal-title":"J Natl Med Assoc"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/20.5.1241"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(07)70286-4"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.21098"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1177\/0300060519847406"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.thromres.2011.11.036"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2013.05.003"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1201103"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/jth.12795"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-06-2202"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.13.104"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2956"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1711.2005.01314.x"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1425"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.thromres.2012.11.028"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/384685"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20112322"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.01000"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2012.06.011"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0000631"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002675"},{"key":"e_1_2_8_26_1","first-page":"e1003167","article-title":"Neutrophil exert a supressive effect on TH1 responses to intracellular pathogen Brucella abortus","volume":"9","author":"Barquero\u2010Calvo E","year":"2013","journal-title":"PLoS One"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.01012"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(03)00075-1"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.23.3.737-742.1979"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/152.5.913"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/89.1.87"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1177\/030098588802500104"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.63.8.3054-3061.1995"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2009.04.010"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.01000"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smim.2016.10.013"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2009.09.016"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0809549"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3024"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.181.10.7121"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1001289"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jit278"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.60.10.4407-4409.1992"},{"key":"e_1_2_8_44_1","first-page":"4756","article-title":"Activated granulocytes and granulocyte\u2010derived hydrogen peroxide are the underlying mechanism of suppression of T\u2010cell function in advanced cancer patients","volume":"61","author":"Schmielau J","year":"2001","journal-title":"Cancer Res"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.112.3.491"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(90)90443-I"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V75.3.550.550"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.128.4.661"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2013.06.004"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2011.11.012"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/0167-5699(95)80066-2"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.1996.tb00136.x"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.20.12.2702"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2010.03.009"},{"key":"e_1_2_8_55_1","first-page":"308","article-title":"Platelet\u2010activating factor\u2010induced polymorphonuclear neutrophil priming independent of CD11(B) adhesion","volume":"114","author":"Read RA","year":"1993","journal-title":"Surgery"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA117.000515"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.166.5.3355"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/dxl119"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.172.8.4956"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-01-069195"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.01086.2009"},{"key":"e_1_2_8_62_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1997.83.3.681"},{"key":"e_1_2_8_63_1","doi-asserted-by":"publisher","DOI":"10.1080\/00365519950185463"},{"key":"e_1_2_8_64_1","doi-asserted-by":"publisher","DOI":"10.1002\/jlb.67.4.471"},{"key":"e_1_2_8_65_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.170.5.1745"},{"key":"e_1_2_8_66_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201108-1533OC"},{"key":"e_1_2_8_67_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1991.tb12351.x"},{"key":"e_1_2_8_68_1","doi-asserted-by":"crossref","first-page":"272","DOI":"10.7883\/yoken.64.272","article-title":"Evaluation of serological tests for diagnosis of brucellosis","volume":"64","author":"Pabuccuoglu O","year":"2011","journal-title":"Jpn J Infect Dis"},{"key":"e_1_2_8_69_1","first-page":"97","volume-title":"Brucellosis Clinical and Laboratory Aspects","author":"Young EJ","year":"1989"},{"key":"e_1_2_8_70_1","first-page":"7","article-title":"Separation of leukocytes from blood and bone marrow. Introduction","volume":"97","author":"B\u00f6yum A","year":"1968","journal-title":"Scand J Clin Lab Investig Suppl"},{"key":"e_1_2_8_71_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.1998.00601.x"}],"container-title":["Immunology &amp; Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fimcb.12373","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/imcb.12373","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/imcb.12373","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/imcb.12373","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,4]],"date-time":"2023-09-04T05:44:54Z","timestamp":1693806294000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/imcb.12373"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,8,4]]},"references-count":70,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2020,10]]}},"alternative-id":["10.1111\/imcb.12373"],"URL":"https:\/\/doi.org\/10.1111\/imcb.12373","archive":["Portico"],"relation":{},"ISSN":["0818-9641","1440-1711"],"issn-type":[{"type":"print","value":"0818-9641"},{"type":"electronic","value":"1440-1711"}],"subject":[],"published":{"date-parts":[[2020,8,4]]},"assertion":[{"value":"2020-03-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-07-03","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-08-04","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}