{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T03:05:00Z","timestamp":1775012700633,"version":"3.50.1"},"reference-count":37,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,1,31]],"date-time":"2021-01-31T00:00:00Z","timestamp":1612051200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunol. Cell Biol."],"published-print":{"date-parts":[[2021,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The RNA\u2010binding protein heterogeneous nuclear ribonucleoprotein L\u2010like (hnRNPLL) controls alternative splicing of protein tyrosine phosphatase receptor type C (<jats:italic>Ptprc<\/jats:italic>) which encodes CD45. hnRNPLL deficiency leads to a failure in silencing <jats:italic>Ptprc<\/jats:italic> exons 4\u20136 causing aberrant expression of the corresponding CD45 isoforms, namely, CD45RA, RB and RC. While an <jats:italic>N<\/jats:italic>\u2010ethyl\u2010<jats:italic>N<\/jats:italic>\u2010nitrosourea\u2010induced point mutation in murine <jats:italic>Hnrnpll<\/jats:italic> results in loss of peripheral na\u00efve T cells, its role in B\u2010cell biology remains unclear. Here, we demonstrate that B\u2010cell development in the bone marrow of <jats:italic>Hnrnpll<\/jats:italic><jats:italic><jats:sup>thu\/thu<\/jats:sup><\/jats:italic> mice is normal and the number of mature B\u2010cell subsets in the spleen and peritoneal cavity is comparable to control littermates. In response to <jats:italic>in vivo<\/jats:italic> immunization, <jats:italic>Hnrnpll<\/jats:italic><jats:italic><jats:sup>thu\/thu<\/jats:sup><\/jats:italic> mice were deficient in generating germinal center (GC) B cells, and analysis of mixed bone marrow chimeras revealed that the GC B\u2010cell deficiency was a B\u2010cell extrinsic effect of the hnRNPLL mutation. Mature <jats:italic>Hnrnpll<\/jats:italic><jats:italic><jats:sup>thu\/thu<\/jats:sup><\/jats:italic> B cells proliferated normally in response to various B\u2010cell receptor\u2010 and Toll\u2010like receptor\u2010mediated stimuli. Similarly, <jats:italic>in vitro<\/jats:italic> stimulation of mutant B cells led to normal generation of plasmablasts, but mutant plasmablasts failed to downregulate B220 expression because of the inability of cells to undergo proper CD45 pre\u2010messenger RNA alternative splicing. These findings collectively suggest that, like in T and natural killer T cells, the mutation disrupts hnRNPLL\u2010mediated alternative splicing of the <jats:italic>Ptprc<\/jats:italic> gene in plasmablasts, but this dysregulation of <jats:italic>Ptprc<\/jats:italic> alternative splicing does not affect the development and function of B cells.<\/jats:p>","DOI":"10.1111\/imcb.12433","type":"journal-article","created":{"date-parts":[[2020,12,18]],"date-time":"2020-12-18T06:40:05Z","timestamp":1608273605000},"page":"532-541","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Loss of hnRNPLL\u2010dependent splicing of <i>Ptprc<\/i> has no impact on B\u2010cell development, activation and terminal differentiation into antibody\u2010secreting cells"],"prefix":"10.1111","volume":"99","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3462-5389","authenticated-orcid":false,"given":"Mehmet","family":"Yabas","sequence":"first","affiliation":[{"name":"Department of Immunology and Infectious Disease The John Curtin School of Medical Research The Australian National University Canberra ACT Australia"},{"name":"Department of Genetics and Bioengineering Trakya University Edirne Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yavuz F","family":"Yazicioglu","sequence":"additional","affiliation":[{"name":"Department of Immunology and Infectious Disease The John Curtin School of Medical Research The Australian National University Canberra ACT Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerard F","family":"Hoyne","sequence":"additional","affiliation":[{"name":"School of Health Sciences Institute of Health Science Research The University of Notre Dame Australia Fremantle WA Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5296-6155","authenticated-orcid":false,"given":"Christopher C","family":"Goodnow","sequence":"additional","affiliation":[{"name":"Department of Immunology and Infectious Disease The John Curtin School of Medical Research The Australian National University Canberra ACT Australia"},{"name":"The Garvan Institute of Medical Research The University of New South Wales Darlinghurst NSW Australia"},{"name":"Department of Medicine St Vincent\u2019s Clinical School University of New South Wales Darlinghurst NSW Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5933-6463","authenticated-orcid":false,"given":"Anselm","family":"Enders","sequence":"additional","affiliation":[{"name":"Department of Immunology and Infectious Disease The John Curtin School of Medical Research The Australian National University Canberra ACT Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,1,31]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.21.120601.140946"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2567.2005.02265.x"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms17010003"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/imr.12047"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2018.01.009"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7600745"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.00419-07"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2008.11.004"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1157610"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1261\/rna.1212008"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks221"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0903625"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0026440"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.neulet.2020.135469"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00005-010-0073-2"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3570"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.3.1800"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(81)90325-1"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1900"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2015.12.008"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.149.7.2286"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1006\/cimm.1993.1224"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1214414109"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1422490112"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni1008-1091"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2008.03.014"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2011.11.001"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-031210-101400"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1175870"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1176676"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2009.07.002"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smim.2011.08.010"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/wrna.1335"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1820"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90030-G"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2011"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2012.02.012"}],"container-title":["Immunology 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