{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T02:46:35Z","timestamp":1772160395174,"version":"3.50.1"},"reference-count":42,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2021,5,20]],"date-time":"2021-05-20T00:00:00Z","timestamp":1621468800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunol. Cell Biol."],"published-print":{"date-parts":[[2021,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Brown adipose tissue (BAT) may be an important metabolic regulator of whole\u2010body glucose. While important roles have been ascribed to macrophages in regulating metabolic functions in BAT, little is known of the roles of other immune cells subsets, particularly dendritic cells (DCs). Eating a high\u2010fat diet may compromise the development of hematopoietic stem and progenitor cells (HSPCs)\u2014which give rise to DCs\u2014in bone marrow, with less known of its effects in BAT. We have previously demonstrated that ongoing exposure to low\u2010dose ultraviolet radiation (UVR) significantly reduced the \u2018whitening\u2019 effect of eating a high\u2010fat diet upon interscapular (i) BAT of mice. Here, we examined whether this observation may be linked to changes in the phenotype of HSPCs and myeloid\u2010derived immune cells in iBAT and bone marrow of mice using 12\u2010colour flow cytometry. Many HSPC subsets declined in both iBAT and bone marrow with increasing metabolic dysfunction. Conversely, with rising adiposity and metabolic dysfunction, conventional DCs (cDCs) increased in both of these tissues. When compared with a low\u2010fat diet, consumption of a high\u2010fat diet significantly reduced proportions of myeloid, common myeloid and megakaryocyte\u2013erythrocyte progenitors in iBAT, and short\u2010term hematopoietic stem cells in bone marrow. In mice fed the high\u2010fat diet, exposure to low\u2010dose UVR significantly reduced proportions of cDCs in iBAT, independently of nitric oxide release from irradiated skin [blocked using the scavenger 2\u2010(4\u2010carboxyphenyl)\u20104,4,5,5\u2010tetramethylimidazoline\u20101\u2010oxyl\u20103\u2010oxide potassium salt (cPTIO)], but did not significantly modify HSPC subsets in either tissue. Further studies are needed to determine whether changes in these cell populations contribute towards metabolic dysfunction<\/jats:p>\n                  <jats:p>.<\/jats:p>","DOI":"10.1111\/imcb.12460","type":"journal-article","created":{"date-parts":[[2021,4,18]],"date-time":"2021-04-18T14:41:12Z","timestamp":1618756872000},"page":"749-766","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Metabolic dysfunction induced by a high\u2010fat diet modulates hematopoietic stem and myeloid progenitor cells in brown adipose tissue of mice"],"prefix":"10.1111","volume":"99","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4419-7258","authenticated-orcid":false,"given":"Kyle T","family":"Mincham","sequence":"first","affiliation":[{"name":"Telethon Kids Institute University of Western Australia Perth 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