{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T06:53:01Z","timestamp":1790837581972,"version":"4.1.0"},"reference-count":76,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,4,11]],"date-time":"2022-04-11T00:00:00Z","timestamp":1649635200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["1142505"],"award-info":[{"award-number":["1142505"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001801","name":"University of Western Australia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001801","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunol Cell Biol"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    A population of neutrophils recruited into cystic fibrosis (CF) airways is associated with proteolytic lung damage, exhibiting high expression of primary granule exocytosis marker CD63 and reduced phagocytic receptor CD16. Causative factors for this population are unknown, limiting intervention. Here we present a laboratory model to characterize responses of differentiated airway epithelium and neutrophils following respiratory infection. Pediatric primary airway epithelial cells were cultured at the air\u2013liquid interface, challenged individually or in combination with rhinovirus (RV) and\n                    <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n                    , then apically washed with medical saline to sample epithelial infection milieus. Cytokine multiplex analysis revealed epithelial antiviral signals, including IP\u201010 and RANTES, increased with exclusive RV infection but were diminished if\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    was also present. Proinflammatory signals interleukin\u20101\u03b1 and \u03b2 were dominant in\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    infection milieus. Infection washes were also applied to a published model of neutrophil transmigration into the airways. Neutrophils migrating into bacterial and viral\u2013bacterial co\u2010infection milieus exhibited the\n                    <jats:italic>in\u2009vivo<\/jats:italic>\n                    CF phenotype of increased CD63 expression and reduced CD16 expression, while neutrophils migrating into milieus of RV\u2010infected or uninfected cultures did not. Individually, bacterial products lipopolysaccharide and\n                    <jats:italic>N<\/jats:italic>\n                    \u2010formylmethionyl\u2010leucyl\u2010phenylalanine and isolated cytokine signals only partially activated this phenotype, suggesting that additional soluble factors in the infection microenvironment trigger primary granule release. Findings identify\n                    <jats:italic>P. aeruginosa<\/jats:italic>\n                    as a trigger of acute airway inflammation and neutrophil primary granule exocytosis, underscoring potential roles of airway microbes in prompting this neutrophil subset. Further studies are required to characterize microbes implicated in primary granule release, and identify potential therapeutic targets.\n                  <\/jats:p>","DOI":"10.1111\/imcb.12547","type":"journal-article","created":{"date-parts":[[2022,3,23]],"date-time":"2022-03-23T03:56:10Z","timestamp":1648007770000},"page":"352-370","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["<i>Pseudomonas aeruginosa<\/i>\n                    modulates neutrophil granule exocytosis in an\n                    <i>in\u2009vitro<\/i>\n                    model of airway infection"],"prefix":"10.1111","volume":"100","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2208-0844","authenticated-orcid":false,"given":"Daniel R","family":"Laucirica","sequence":"first","affiliation":[{"name":"Faculty of Health and Medical Sciences University of Western Australia  Nedlands WA Australia"},{"name":"Wal\u2010Yan Respiratory Research Centre Telethon Kids Institute University of Western Australia  Nedlands WA Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9771-3194","authenticated-orcid":false,"given":"Craig J","family":"Schofield","sequence":"additional","affiliation":[{"name":"Wal\u2010Yan Respiratory Research Centre Telethon Kids Institute University of Western Australia  Nedlands WA Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0189-534X","authenticated-orcid":false,"given":"Samantha A","family":"McLean","sequence":"additional","affiliation":[{"name":"Wal\u2010Yan Respiratory Research Centre Telethon Kids Institute University of Western Australia  Nedlands WA Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3952-0778","authenticated-orcid":false,"given":"Camilla","family":"Margaroli","sequence":"additional","affiliation":[{"name":"Department of Medicine Division of Pulmonary, Allergy and Critical Care Medicine University of Alabama at Birmingham  Birmingham AL USA"},{"name":"Program in Protease and Matrix Biology University of Alabama at Birmingham  Birmingham AL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3703-4111","authenticated-orcid":false,"given":"Patricia","family":"Agudelo\u2010Romero","sequence":"additional","affiliation":[{"name":"Wal\u2010Yan Respiratory Research Centre Telethon Kids Institute University of Western Australia  Nedlands WA Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5386-8482","authenticated-orcid":false,"given":"Stephen M","family":"Stick","sequence":"additional","affiliation":[{"name":"Faculty of Health and Medical Sciences University of Western Australia  Nedlands WA Australia"},{"name":"Wal\u2010Yan Respiratory Research Centre Telethon Kids Institute University of Western Australia  Nedlands WA Australia"},{"name":"Department of Respiratory and Sleep Medicine Perth Children\u2019s Hospital  Nedlands WA Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3526-9985","authenticated-orcid":false,"given":"Rabindra","family":"Tirouvanziam","sequence":"additional","affiliation":[{"name":"Department of Pediatrics Emory University  Atlanta GA USA"},{"name":"Center for CF and Airways Disease Research Children\u2019s Healthcare of Atlanta  Atlanta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0008-9733","authenticated-orcid":false,"given":"Anthony","family":"Kicic","sequence":"additional","affiliation":[{"name":"Faculty of Health and Medical Sciences University of Western Australia  Nedlands WA Australia"},{"name":"Wal\u2010Yan Respiratory Research Centre Telethon Kids Institute University of Western Australia  Nedlands WA Australia"},{"name":"Department of Respiratory and Sleep Medicine Perth Children\u2019s Hospital  Nedlands WA Australia"},{"name":"Occupation and Environment School of Public Health Curtin University  Bentley WA Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9381-326X","authenticated-orcid":false,"given":"Luke W","family":"Garratt","sequence":"additional","affiliation":[{"name":"Wal\u2010Yan Respiratory Research Centre Telethon Kids Institute University of Western Australia  Nedlands WA Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Western Australian Epithelial Research Program (WAERP)","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Australian Respiratory Early Surveillance Team for CF (AREST CF)","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,4,11]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004651"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40348-016-0066-2"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcf.2012.07.003"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201203-0507OC"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.01771-2018"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1301725"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/JLB.5HI1117-454RR"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/JLB.5CE0418-162R"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0712386105"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0813410106"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201803-0442OC"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xcrm.2021.100239"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-39726-w"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.01438"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00066.2004"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcf.2005.12.003"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2010-0368OC"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.00596"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12931-017-0706-7"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200207-765OC"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/jlb.67.5.651"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.00869-12"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI113887"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00477.2004"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.2000.279.6.L1083"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1750-2659.2011.00292.x"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcf.2020.12.015"},{"key":"e_1_2_8_29_1","volume-title":"Guidance for Industry: Pyrogen and Endotoxins Testing: Questions and Answers","author":"FDA","year":"2012"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2017.00507"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.3HI0716-293RR"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.05120-11"},{"key":"e_1_2_8_33_1","first-page":"4343","article-title":"Pseudomonas aeruginosa influences the inflammatory response of primary bronchial epithelial cells to rhinovirus infection","volume":"56","author":"Endres A","year":"2020","journal-title":"Eur Respir J"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.00096"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2007-0050OC"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12931-019-1120-0"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12890-016-0339-5"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.00769-2020"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1183\/09031936.00043108"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1378\/chest.15-0676"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006798"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcf.2018.05.006"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1102048"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/imr.12621"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10753-018-0780-0"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201609-1830OC"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201905-1013OC"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0114884"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/JLB.3AB0321-149R"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3806"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.10870"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1177\/1753425916668980"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/3021863"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1139\/cjpp-2015-0219"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.110.068064"},{"key":"e_1_2_8_56_1","first-page":"CD001505","article-title":"Oral non\u2010steroidal anti\u2010inflammatory drug therapy for lung disease in cystic fibrosis","volume":"9","author":"Lands LC","year":"2019","journal-title":"Cochrane Database Syst Rev"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcf.2015.03.007"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/163.1.89"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1567-5769(03)00179-6"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.290.13.1749"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpeds.2008.08.001"},{"key":"e_1_2_8_62_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201407-1277OC"},{"key":"e_1_2_8_63_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201810-1919OC"},{"key":"e_1_2_8_64_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201908-1585OC"},{"key":"e_1_2_8_65_1","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.00832-2017"},{"key":"e_1_2_8_66_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.00595"},{"key":"e_1_2_8_67_1","doi-asserted-by":"publisher","DOI":"10.3390\/v12080808"},{"key":"e_1_2_8_68_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-76781-0"},{"key":"e_1_2_8_69_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2017.00160"},{"key":"e_1_2_8_70_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbi.2019.103208"},{"key":"e_1_2_8_71_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbi.2008.08.010"},{"key":"e_1_2_8_72_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-17952-4"},{"key":"e_1_2_8_73_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00368.2013"},{"key":"e_1_2_8_74_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-1571-2_5"},{"key":"e_1_2_8_75_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-1571-2_7"},{"key":"e_1_2_8_76_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2358"},{"key":"e_1_2_8_77_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-50094-3"}],"container-title":["Immunology &amp; Cell Biology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/imcb.12547","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/imcb.12547","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/imcb.12547","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,25]],"date-time":"2023-08-25T14:18:48Z","timestamp":1692973128000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/imcb.12547"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,4,11]]},"references-count":76,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2022,5]]}},"alternative-id":["10.1111\/imcb.12547"],"URL":"https:\/\/doi.org\/10.1111\/imcb.12547","archive":["Portico"],"relation":{},"ISSN":["0818-9641","1440-1711"],"issn-type":[{"value":"0818-9641","type":"print"},{"value":"1440-1711","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,4,11]]},"assertion":[{"value":"2021-12-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-03-21","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-04-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}