{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,2]],"date-time":"2026-04-02T15:13:26Z","timestamp":1775142806196,"version":"3.50.1"},"reference-count":64,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2022,6,21]],"date-time":"2022-06-21T00:00:00Z","timestamp":1655769600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["1068040"],"award-info":[{"award-number":["1068040"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunol Cell Biol"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Studies have highlighted a critical role for autophagy in the regulation of multiple cytokines. Autophagy inhibits the release of interleukin (IL)\u20101 family cytokines, including IL\u20101\u03b1, IL\u20101\u03b2 and IL\u201018, by myeloid cells. This, in turn, impacts the release of other cytokines by myeloid cells, as well as other cells of the immune system, including IL\u201022, IL\u201023, IL\u201017 and interferon\u2010\u03b3. Here, we assessed the impact of genetic depletion of the autophagy gene <jats:italic>Atg7<\/jats:italic> in myeloid cells on acute and chronic inflammation. In a model of acute lipopolysaccharide\u2010induced endotoxemia, loss of autophagy in myeloid cells resulted in increased release of proinflammatory cytokines, both locally and systemically. By contrast, loss of <jats:italic>Atg7<\/jats:italic> in myeloid cells in the <jats:italic>Lyn<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> model of lupus\u2010like autoimmunity resulted in reduced systemic release of IL\u20106 and IL\u201010, with no effects on other cytokines observed. In addition, <jats:italic>Lyn<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> mice with autophagy\u2010deficient myeloid cells showed reduced expression of autoantibodies relevant to systemic lupus erythematosus, including anti\u2010histone and anti\u2010Smith protein. <jats:italic>In vitro<\/jats:italic>, loss of autophagy, through pharmacological inhibition or small interfering RNA against <jats:italic>Becn1<\/jats:italic>, inhibited IL\u201010 release by human and mouse myeloid cells. This effect was evident at the level of <jats:italic>Il10<\/jats:italic> messenger RNA expression. Our data highlight potentially important differences in the role of myeloid cell autophagy in acute and chronic inflammation and demonstrate a direct role for autophagy in the production and release of IL\u201010 by macrophages.<\/jats:p>","DOI":"10.1111\/imcb.12562","type":"journal-article","created":{"date-parts":[[2022,6,2]],"date-time":"2022-06-02T09:04:35Z","timestamp":1654160675000},"page":"605-623","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Investigating immunoregulatory effects of myeloid cell autophagy in acute and chronic inflammation"],"prefix":"10.1111","volume":"100","author":[{"given":"Md Abul","family":"Hasnat","sequence":"first","affiliation":[{"name":"Centre for Inflammatory Diseases, Department of Medicine School of Clinical Sciences at Monash Health Faculty of Medicine, Nursing and Health Sciences Monash University  Clayton VIC 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