{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T15:44:42Z","timestamp":1764603882977},"reference-count":39,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2022,6,26]],"date-time":"2022-06-26T00:00:00Z","timestamp":1656201600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81870091","81901643"],"award-info":[{"award-number":["81870091","81901643"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunol Cell Biol"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Humanized mice reconstituted with a functional human immune system (HIS) are instrumental in studying human immunity and immune disorders <jats:italic>in\u2009vivo<\/jats:italic>. The poor or lack of cross\u2010reactivity between mouse cytokines and human cells limits the development and\/or function of human immune cell subsets including human myeloid, natural killer and B cells. Here we explored the potential to achieve long\u2010term production of human cytokines in immunodeficient mice using a transposon\u2010plasmid\u2013based hydrodynamic injection approach. We constructed a transposon\u2010plasmid carrying five human cytokine coding sequences (named PB\u20105F), and observed that four of the cytokines (granulocyte\u2013macrophage colony\u2010stimulating factor, interleukin (IL)\u201015, IL\u20106 and IL\u20103) were detectable in sera and three (granulocyte\u2013macrophage colony\u2010stimulating factor, IL\u201015 and IL\u20106) showed long\u2010term production in immunodeficient mice that received a single hydrodynamic injection of PB\u20105F plus the transposase plasmid (Super PB). Furthermore, a single injection of PB\u20105F\/Super PB markedly enhanced the reconstitution of human myeloid cells and natural killer cells, and promoted human B\u2010cell maturation in HIS mice. Taken together, our data revealed that hydrodynamic injection of the PB\u20105F\/Super PB vectors may serve as a convenient and efficacious means to promote human immune function in HIS mice.<\/jats:p>","DOI":"10.1111\/imcb.12564","type":"journal-article","created":{"date-parts":[[2022,6,4]],"date-time":"2022-06-04T14:54:30Z","timestamp":1654354470000},"page":"624-635","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Improvement of human myeloid and natural killer cell development in humanized mice <i>via<\/i> hydrodynamic injection of transposon plasmids containing multiple human cytokine genes"],"prefix":"10.1111","volume":"100","author":[{"given":"Shulian","family":"Tan","sequence":"first","affiliation":[{"name":"Key Laboratory of Organ Regeneration &amp; Transplantation of Ministry of Education The First Hospital of Jilin University  Changchun China"},{"name":"National\u2010Local Joint Engineering Laboratory of Animal Models for Human Diseases The First Hospital of Jilin University  Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Minghui","family":"Fang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Organ Regeneration &amp; Transplantation of Ministry of Education The First Hospital of Jilin University  Changchun China"},{"name":"National\u2010Local Joint Engineering Laboratory of Animal Models for Human Diseases The First Hospital of Jilin University  Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Fan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Organ Regeneration &amp; Transplantation of Ministry of Education The First Hospital of Jilin University  Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhaowei","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Organ Regeneration &amp; Transplantation of Ministry of Education The First Hospital of Jilin University  Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanan","family":"Lv","sequence":"additional","affiliation":[{"name":"Key Laboratory of Organ Regeneration &amp; Transplantation of Ministry of Education The First Hospital of Jilin University  Changchun China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Zou","sequence":"additional","affiliation":[{"name":"Key Laboratory of Organ Regeneration &amp; 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