{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T00:31:44Z","timestamp":1783643504396,"version":"3.55.0"},"reference-count":37,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,1,29]],"date-time":"2023-01-29T00:00:00Z","timestamp":1674950400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000009","name":"Foundation for the National Institutes of Health","doi-asserted-by":"publisher","award":["5R01AI114728"],"award-info":[{"award-number":["5R01AI114728"]}],"id":[{"id":"10.13039\/100000009","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003977","name":"Israel Science Foundation","doi-asserted-by":"publisher","award":["882\/17"],"award-info":[{"award-number":["882\/17"]}],"id":[{"id":"10.13039\/501100003977","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunol Cell Biol"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Vaccination and natural infection both elicit potent humoral responses that provide protection from subsequent infections. The immune history of an individual following such exposures is in part encoded by antibodies. While there are multiple immunoassays for measuring antibody responses, the majority of these methods measure responses to a single antigen. A commonly used method for measuring antibody responses is ELISA\u2014a semiquantitative assay that is simple to perform in research and clinical settings. Here, we present FLU\u2010LISA (fluorescence\u2010linked immunosorbent assay)\u2014a novel antigen microarray\u2010based assay for rapid high\u2010throughput antibody profiling. The assay can be used for profiling immunoglobulin (Ig) G, IgA and IgM responses to multiple antigens simultaneously, requiring minimal amounts of sample and antigens. Using several influenza and severe acute respiratory syndrome coronavirus 2 (SARS\u2010CoV\u20102) antigen microarrays, we demonstrated the specificity and sensitivity of our novel assay and compared it with the traditional ELISA, using samples from mice, chickens and humans. We also showed that our assay can be readily used with dried blood spots, which can be collected from humans and wild birds. FLU\u2010LISA can be readily used to profile hundreds of samples against dozens of antigens in a single day, and therefore offers an attractive alternative to the traditional ELISA.<\/jats:p>","DOI":"10.1111\/imcb.12618","type":"journal-article","created":{"date-parts":[[2022,12,26]],"date-time":"2022-12-26T06:19:08Z","timestamp":1672035548000},"page":"231-248","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["<scp>FLU\u2010LISA (<\/scp>fluorescence\u2010linked immunosorbent assay<scp>)<\/scp>: high\u2010throughput antibody profiling using antigen microarrays"],"prefix":"10.1111","volume":"101","author":[{"given":"Shlomia","family":"Levy","sequence":"first","affiliation":[{"name":"The Shraga Segal Department of Microbiology and Immunology Ben\u2010Gurion University of the Negev  Beer\u2010Sheva Israel"},{"name":"National Institute of Biotechnology in the Negev  Beer\u2010Sheva Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marwa","family":"Abd Alhadi","sequence":"additional","affiliation":[{"name":"The Shraga Segal Department of Microbiology and Immunology Ben\u2010Gurion University of the Negev  Beer\u2010Sheva Israel"},{"name":"National Institute of Biotechnology in the Negev  Beer\u2010Sheva Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asaf","family":"Azulay","sequence":"additional","affiliation":[{"name":"The Shraga Segal Department of Microbiology and Immunology Ben\u2010Gurion University of the Negev  Beer\u2010Sheva Israel"},{"name":"National Institute of Biotechnology in the Negev  Beer\u2010Sheva Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amit","family":"Kahana","sequence":"additional","affiliation":[{"name":"The Shraga Segal Department of Microbiology and Immunology Ben\u2010Gurion University of the Negev  Beer\u2010Sheva Israel"},{"name":"National Institute of Biotechnology in the Negev  Beer\u2010Sheva Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nir","family":"Bujanover","sequence":"additional","affiliation":[{"name":"The Shraga Segal Department of Microbiology and Immunology Ben\u2010Gurion University of the Negev  Beer\u2010Sheva Israel"},{"name":"National Institute of Biotechnology in the Negev  Beer\u2010Sheva Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roi","family":"Gazit","sequence":"additional","affiliation":[{"name":"The Shraga Segal Department of Microbiology and Immunology Ben\u2010Gurion University of the Negev  Beer\u2010Sheva Israel"},{"name":"National Institute of Biotechnology in the Negev  Beer\u2010Sheva Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0916-0372","authenticated-orcid":false,"given":"Maureen A","family":"McGargill","sequence":"additional","affiliation":[{"name":"Department of Immunology St. Jude Children's Research Hospital  Memphis TN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lilach M","family":"Friedman","sequence":"additional","affiliation":[{"name":"The Shraga Segal Department of Microbiology and Immunology Ben\u2010Gurion University of the Negev  Beer\u2010Sheva Israel"},{"name":"National Institute of Biotechnology in the Negev  Beer\u2010Sheva Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0561-1578","authenticated-orcid":false,"given":"Tomer","family":"Hertz","sequence":"additional","affiliation":[{"name":"The Shraga Segal Department of Microbiology and Immunology Ben\u2010Gurion University of the Negev  Beer\u2010Sheva Israel"},{"name":"National Institute of Biotechnology in the Negev  Beer\u2010Sheva Israel"},{"name":"Vaccine and Infectious Disease Division Fred Hutch Cancer Research Center  Seattle WA 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