{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T13:18:32Z","timestamp":1774703912604,"version":"3.50.1"},"reference-count":58,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,2,14]],"date-time":"2023-02-14T00:00:00Z","timestamp":1676332800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunol Cell Biol"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Genital <jats:italic>Chlamydia trachomatis<\/jats:italic> infection remains a major health issue as it causes severe complications including pelvic inflammatory disease, ectopic pregnancy and infertility in females as a result of infection\u2010associated chronic inflammation. Podoplanin, a transmembrane receptor, has been previously reported on inflammatory macrophages. Thus, strategies that specifically target podoplanin might be able to reduce local inflammation. This study investigated the expression level and function of podoplanin in a <jats:italic>C.\u00a0trachomatis<\/jats:italic> infection model. C57BL\/6 mice infected with the mouse pathogen <jats:italic>Chlamydia muridarum<\/jats:italic> were examined intermittently from days 1 to 60 using flow cytometry analysis. Percentages of conventional macrophages (CD11b<jats:sup>+<\/jats:sup>CD11c<jats:sup>\u2212<\/jats:sup>F4\/80<jats:sup>+<\/jats:sup>) <jats:italic>versus<\/jats:italic> inflammatory macrophages (CD11b<jats:sup>+<\/jats:sup>CD11c<jats:sup>+<\/jats:sup>F4\/80<jats:sup>+<\/jats:sup>), and the expression of podoplanin in these cells were investigated. Subsequently, a podoplanin\u2010knockout RAW264.7 cell was used to evaluate the function of podoplanin in <jats:italic>C.\u00a0trachomatis<\/jats:italic> infection. Our findings demonstrated an increased CD11b<jats:sup>+<\/jats:sup> cell volume in the spleen at day 9 after the infection, with augmented podoplanin expression, especially among the inflammatory macrophages. A large number of podoplanin\u2010expressing macrophages were detected in the genital tract of <jats:italic>C.\u00a0muridarum<\/jats:italic>\u2013infected mice. Furthermore, analysis of the <jats:italic>C.\u00a0trachomatis<\/jats:italic>\u2013infected patients demonstrated a higher percentage of podoplanin\u2010expressing monocytes than that in the noninfected controls. Using an <jats:italic>in\u00a0vitro<\/jats:italic> infection in a transwell migration assay, we identified that macrophages deficient in podoplanin displayed defective migratory function toward <jats:italic>C.\u00a0trachomatis<\/jats:italic>\u2013infected HeLa 229 cells. Lastly, using immunoprecipitation\u2013mass spectrometry method, we identified two potential podoplanin interacting proteins, namely, Cofilin 1 and Talin 1 actin\u2010binding proteins. The present study reports a role of podoplanin in directing macrophage migration to the chlamydial infection site. Our results suggest a potential for reducing inflammation in individuals with chronic chlamydial infections by targeting podoplanin.<\/jats:p>","DOI":"10.1111\/imcb.12621","type":"journal-article","created":{"date-parts":[[2023,1,20]],"date-time":"2023-01-20T04:03:31Z","timestamp":1674187411000},"page":"305-320","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Influx of podoplanin\u2010expressing inflammatory macrophages into the genital tract following <i>Chlamydia<\/i>\u00a0infection"],"prefix":"10.1111","volume":"101","author":[{"given":"Yee Teng","family":"Chan","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology, Faculty of Medicine University of Malaya  Kuala Lumpur Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi Ying","family":"Cheok","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Faculty of Medicine University of Malaya  Kuala Lumpur Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heng Choon","family":"Cheong","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Faculty of Medicine University of Malaya  Kuala Lumpur Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Grace Min Yi","family":"Tan","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Faculty of Medicine University of Malaya  Kuala Lumpur Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shi Rui","family":"Seow","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Faculty of Medicine University of Malaya  Kuala Lumpur Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ting Fang","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Faculty of Medicine University of Malaya  Kuala Lumpur Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sofiah","family":"Sulaiman","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Faculty of Medicine University of Malaya  Kuala Lumpur Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chung Yeng","family":"Looi","sequence":"additional","affiliation":[{"name":"School of Bioscience, Faculty of Health &amp; Medical Sciences Taylor's University  Subang Jaya Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rishein","family":"Gupta","sequence":"additional","affiliation":[{"name":"Center of Excellence in Infection Genomics, South Texas Center for Emerging Infectious Diseases University of Texas at San Antonio  San\u00a0Antonio TX USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernard","family":"Arulanandam","sequence":"additional","affiliation":[{"name":"Center of Excellence in Infection Genomics, South Texas 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