{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T02:18:08Z","timestamp":1777515488749,"version":"3.51.4"},"reference-count":51,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,2,27]],"date-time":"2023-02-27T00:00:00Z","timestamp":1677456000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["APP1143154"],"award-info":[{"award-number":["APP1143154"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunol Cell Biol"],"published-print":{"date-parts":[[2023,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Many interferon (IFN)\u2010stimulated genes are upregulated within host cells following infection with influenza and other viruses. While the antiviral activity of some IFN\u2010stimulated genes, such as the IFN\u2010inducible GTPase myxoma resistance (Mx)1\u00a0protein 1, has been well defined, less is known regarding the antiviral activities of related IFN\u2010inducible GTPases of the guanylate\u2010binding protein (GBP) family, particularly mouse GBPs, where mouse models can be used to assess their antiviral properties <jats:italic>in vivo<\/jats:italic>. Herein, we demonstrate that mouse GBP1 (mGBP1) was upregulated in a mouse airway epithelial cell line (LA\u20104 cells) following pretreatment with mouse IFN\u03b1 or infection by influenza A virus (IAV). Whereas doxycycline\u2010inducible expression of mouse Mx1 (mMx1) in LA\u20104 cells resulted in reduced susceptibility to IAV infection and reduced viral growth, inducible mGBP1 did not. Moreover, primary cells isolated from mGBP1\u2010deficient mice (mGBP1<jats:sup>\u2212\/\u2212<\/jats:sup>) showed no difference in susceptibility to IAV and mGBP1<jats:sup>\u2212\/\u2212<\/jats:sup> macrophages showed no defect in IAV\u2010induced NLRP3 (NLR family pyrin domain containing 3) inflammasome activation. After intranasal IAV infection, mGBP1<jats:sup>\u2212\/\u2212<\/jats:sup> mice also showed no differences in virus replication or induction of inflammatory responses in the airways during infection. Thus, using complementary approaches such as mGBP1 overexpression, cells from mGBP1<jats:sup>\u2212\/\u2212<\/jats:sup> mice and intranasal infection of mGBP1<jats:sup>\u2212\/\u2212<\/jats:sup> we demonstrate that mGBP1 does not play a major role in modulating IAV infection <jats:italic>in vitro<\/jats:italic> or <jats:italic>in vivo<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/imcb.12627","type":"journal-article","created":{"date-parts":[[2023,2,6]],"date-time":"2023-02-06T13:01:18Z","timestamp":1675688478000},"page":"383-396","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Mouse guanylate\u2010binding protein 1 does not mediate antiviral activity against influenza virus <i>in vitro<\/i> or <i>in vivo<\/i>"],"prefix":"10.1111","volume":"101","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3619-9800","authenticated-orcid":false,"given":"Melkamu 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Institute for Infection and Immunity, University of Melbourne  Melbourne VIC Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Si Ming","family":"Man","sequence":"additional","affiliation":[{"name":"Division of Immunology and Infectious Disease, The John Curtin School of Medical Research The Australian National University  Canberra ACT Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarah L","family":"Londrigan","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology The Peter Doherty Institute for Infection and Immunity, University of Melbourne  Melbourne VIC Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick C","family":"Reading","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology The Peter Doherty Institute for Infection and Immunity, University of Melbourne  Melbourne VIC Australia"},{"name":"WHO Collaborating Centre for 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