{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T01:18:05Z","timestamp":1747185485112,"version":"3.40.5"},"reference-count":38,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,6,10]],"date-time":"2024-06-10T00:00:00Z","timestamp":1717977600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["1128416","1041897","1084706"],"award-info":[{"award-number":["1128416","1041897","1084706"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunol Cell Biol"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>CD8<jats:sup>+<\/jats:sup> T\u00a0cells recognizing their cognate antigen are typically recruited as a polyclonal population consisting of multiple clonotypes with varying T\u2010cell receptor (TCR) affinity to the target peptide\u2013major histocompatibility complex (pMHC) complex. Advances in single\u2010cell sequencing have increased accessibility toward identifying TCRs with matched antigens. Here we present the discovery of a monoclonal CD8<jats:sup>+<\/jats:sup> T\u2010cell population with specificity for a hepatitis C virus (HCV)\u2013derived human leukocyte antigen (HLA) class I epitope (HLA\u2010B*07:02 <jats:italic>GPRLGVRAT<\/jats:italic>) which was isolated directly <jats:italic>ex vivo<\/jats:italic> from an individual with an episode of acutely resolved HCV infection. This population was absent before infection and underwent expansion and stable maintenance for at least 2\u2009years after infection as measured by HLA\u2010multimer staining. Furthermore, the monoclonal clonotype was characterized by an unusually long dissociation time (half\u2010life\u2009=\u2009794\u2009s and k<jats:sub>off<\/jats:sub>\u2009=\u20095.73\u2009\u00d7\u200910<jats:sup>\u22124<\/jats:sup>) for its target antigen when compared with previously published results. A comparison with related populations of HCV\u2010specific populations derived from\u00a0the same individual and a second individual suggested that high\u2010affinity TCR\u2013pMHC interactions may be inherent to epitope identity and shape the phenotype of responses which has implications for rational TCR selection and design in the age of personalized immunotherapies.<\/jats:p>","DOI":"10.1111\/imcb.12791","type":"journal-article","created":{"date-parts":[[2024,6,10]],"date-time":"2024-06-10T09:19:09Z","timestamp":1718011149000},"page":"630-641","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Discovery of a monoclonal, high\u2010affinity CD8<sup>+<\/sup> T\u2010cell clone following natural hepatitis C virus infection"],"prefix":"10.1111","volume":"102","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3490-4361","authenticated-orcid":false,"given":"Curtis","family":"Cai","sequence":"first","affiliation":[{"name":"School of Biomedical Sciences, Faculty of Health and Medicine UNSW Sydney  Sydney NSW Australia"},{"name":"The Kirby Institute, Faculty of Health and Medicine UNSW Sydney  Sydney NSW Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth","family":"Keoshkerian","sequence":"additional","affiliation":[{"name":"The Kirby Institute, Faculty of Health and Medicine UNSW Sydney  Sydney NSW Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristof","family":"Wing","sequence":"additional","affiliation":[{"name":"School of Medicine and Health, Institute for Medical Microbiology, Immunology and Hygiene Technical University of Munich  Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6557-9373","authenticated-orcid":false,"given":"Jerome","family":"Samir","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences, Faculty of Health and Medicine UNSW Sydney  Sydney NSW Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manuel","family":"Effenberger","sequence":"additional","affiliation":[{"name":"School of Medicine and Health, Institute for Medical Microbiology, Immunology and Hygiene Technical University of Munich  Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kilian","family":"Schober","sequence":"additional","affiliation":[{"name":"Mikrobiologisches Institut \u2013 Klinische Mikrobiologie, Immunologie und Hygiene Friedrich\u2010Alexander\u2010Universit\u00e4t Erlangen\u2010N\u00fcrnberg  Erlangen Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9844-3744","authenticated-orcid":false,"given":"Rowena A","family":"Bull","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences, Faculty of Health and Medicine UNSW Sydney  Sydney NSW Australia"},{"name":"The Kirby Institute, Faculty of Health and Medicine UNSW Sydney  Sydney NSW Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew R","family":"Lloyd","sequence":"additional","affiliation":[{"name":"The Kirby Institute, Faculty of Health and Medicine UNSW Sydney  Sydney NSW Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dirk H","family":"Busch","sequence":"additional","affiliation":[{"name":"School of Medicine and Health, Institute for Medical Microbiology, Immunology and Hygiene Technical University of Munich  Munich Germany"},{"name":"German Center for Infection Research (Deutsches Zentrum f\u00fcr Infektionsforschung), Partner Site Munich  Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabio","family":"Luciani","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences, Faculty of Health and Medicine UNSW Sydney  Sydney NSW Australia"},{"name":"The Kirby Institute, Faculty of Health and Medicine UNSW Sydney  Sydney NSW Australia"},{"name":"Cellular Genomics Future Institute UNSW Sydney  Sydney NSW Australia"},{"name":"Department of Physiology and Biophysics Weill Cornell Medical College  New York City NY 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