{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T01:18:08Z","timestamp":1747185488680,"version":"3.40.5"},"reference-count":39,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2024,10,30]],"date-time":"2024-10-30T00:00:00Z","timestamp":1730246400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100009844","name":"PA Research Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009844","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100015539","name":"Australian Government","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100015539","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001793","name":"Queensland University of Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001793","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunol Cell Biol"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Treatments targeting the immune system only benefit a subset of patients with bladder cancer (BC). Biomarkers predictive of BC progression and response to specific therapeutic interventions are required. We evaluated whether peripheral blood immune subsets and expression of clinically relevant immune checkpoint markers are associated with clinicopathologic features of BC. Peripheral blood mononuclear cells isolated from blood collected from 23\u2009patients with BC and 9 age\u2010matched unaffected\u2010by\u2010cancer control donors were assessed using a 21\u2010parameter flow cytometry panel composed of markers of T, B, natural killer and myeloid populations and immune checkpoint markers. Patients with BC had significantly lower numbers of circulating CD19<jats:sup>+<\/jats:sup> B cells and elevated circulating CD4<jats:sup>+<\/jats:sup>CD8<jats:sup>+<\/jats:sup> T\u00a0cells compared with the control cohort. Immune checkpoint markers programmed cell death protein 1 (PD\u20101) and T\u2010cell immunoglobulin and mucin\u2010domain containing\u20103 (TIM\u20103) were elevated in the total peripheral immune cell population in patients with BC. Within the BC cohort, PD\u20101 expression in T and myeloid cells was elevated in muscle\u2010invasive compared with non\u2013muscle\u2010invasive disease. In addition, elevated T, B and myeloid PD\u20101 cell surface expression was significantly associated with tumor stage, suggesting that measures of peripheral immune cell exhaustion may be a predictor of tumor progression in BC. Finally, positive correlations between expression levels of the various immune checkpoints both overall and within key peripheral blood immune subsets collected from patients with BC were observed, highlighting likely coregulation of peripheral immune checkpoint expression. The peripheral blood immunophenotype in patients with BC is altered compared with cancer\u2010free individuals. Understanding this dysregulated immune profile will contribute to the identification of diagnostic and prognostic indicators to guide effective immune\u2010targeted, personalized treatments.<\/jats:p>","DOI":"10.1111\/imcb.12829","type":"journal-article","created":{"date-parts":[[2024,10,30]],"date-time":"2024-10-30T15:04:35Z","timestamp":1730300675000},"page":"949-962","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Altered immunophenotypic expression in the peripheral bladder cancer immune landscape"],"prefix":"10.1111","volume":"102","author":[{"given":"Nathan J","family":"Mackenzie","sequence":"first","affiliation":[{"name":"Queensland University of Technology (QUT), School of Biomedical Sciences at Translational Research Institute (TRI)  Brisbane QLD Australia"},{"name":"Queensland Bladder Cancer Initiative (QBCI)  Brisbane QLD Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kate","family":"Zimmermann","sequence":"additional","affiliation":[{"name":"Queensland University of Technology (QUT), School of Biomedical Sciences at Translational Research Institute (TRI)  Brisbane QLD Australia"},{"name":"Centre for Immunology and Infection Control Queensland University of Technology (QUT)  Brisbane QLD Australia"},{"name":"Centre for Microbiome Research Queensland University of Technology (QUT)  Brisbane QLD Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Clarissa","family":"Nicholls","sequence":"additional","affiliation":[{"name":"Queensland University of Technology (QUT), School of Biomedical Sciences at Translational Research Institute (TRI)  Brisbane QLD Australia"},{"name":"Queensland Bladder Cancer Initiative (QBCI)  Brisbane QLD Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahasha 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Institute (TRI)  Brisbane QLD Australia"},{"name":"Queensland Bladder Cancer Initiative (QBCI)  Brisbane QLD Australia"},{"name":"Centre for Personalised Analysis of Cancers (CPAC)  Brisbane QLD Australia"},{"name":"Australian Prostate Cancer Research Centre \u2013 Queensland (APCRC\u2010Q)  Brisbane QLD Australia"},{"name":"Department of Urology Princess Alexandra Hospital  Brisbane QLD Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tony J","family":"Kenna","sequence":"additional","affiliation":[{"name":"Queensland University of Technology (QUT), School of Biomedical Sciences at Translational Research Institute (TRI)  Brisbane QLD Australia"},{"name":"Centre for Immunology and Infection Control Queensland University of Technology (QUT)  Brisbane QLD Australia"},{"name":"Centre for Microbiome Research Queensland University of Technology (QUT)  Brisbane QLD 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