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Many subtypes of macrophages have been identified, classified by their niche and\/or embryonic origin. In order to better develop therapies for conditions with macrophage dysfunction, it is crucial to decipher potential sex differences in key physiological mediators of inflammation so that treatment efficacy can be ensured regardless of biological sex. Here, we conduct a meta\u2010analysis approach of transcriptomics data sets for male vs. female mouse macrophages across 8 niches to characterize conserved sex\u2010dimorphic pathways in macrophages across origins and niches. For this purpose, we leveraged new and publicly available RNA\u2010sequencing data sets from murine macrophages, preprocessed these datasets and filtered them based on objective QC criteria, and performed differential gene expression analysis using sex as the covariate of interest. Differentially expressed (DE) genes were compared across data sets and macrophage subsets, and functional enrichment analysis was performed to identify sex\u2010specific functional differences. Consistent with their presence on the sex chromosomes, three genes were found differentially expressed across datasets (i.e.\n                    <jats:italic>Xist<\/jats:italic>\n                    ,\n                    <jats:italic>Eif2s3y<\/jats:italic>\n                    and\n                    <jats:italic>Ddx3y<\/jats:italic>\n                    ). More broadly, we found that female\u2010biased pathways across niches are more consistent than male\u2010biased pathways, specifically relating to the extracellular matrix. Our findings increase our understanding of transcriptional similarities across macrophage niches and underscore the importance of including sex as a biological variable in immune\u2010related studies.\n                    <jats:inline-graphic xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"graphic\/imcb70030-gra-0001.png\" xlink:title=\"image\"\/>\n                  <\/jats:p>","DOI":"10.1111\/imcb.70030","type":"journal-article","created":{"date-parts":[[2025,5,20]],"date-time":"2025-05-20T05:39:28Z","timestamp":1747719568000},"page":"563-577","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Sex\u2010dimorphic gene regulation in murine macrophages across niches"],"prefix":"10.1111","volume":"103","author":[{"given":"Cassandra J","family":"McGill","sequence":"first","affiliation":[{"name":"Leonard Davis School of Gerontology University of Southern California  Los Angeles CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivia S","family":"White","sequence":"additional","affiliation":[{"name":"Leonard Davis School of Gerontology University of Southern California  Los Angeles CA USA"},{"name":"Quantitative and Computational Biology Department USC Dornsife College of Letters, Arts, and Sciences  Los Angeles CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryan J","family":"Lu","sequence":"additional","affiliation":[{"name":"Leonard Davis School of Gerontology University of Southern California  Los Angeles CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nirmal K","family":"Sampathkumar","sequence":"additional","affiliation":[{"name":"Leonard Davis School of Gerontology University of Southern California  Los Angeles CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7401-4777","authenticated-orcid":false,"given":"B\u00e9r\u00e9nice A","family":"Benayoun","sequence":"additional","affiliation":[{"name":"Leonard Davis School of Gerontology University of Southern California  Los Angeles CA USA"},{"name":"Molecular and Computational Biology Department USC Dornsife College of Letters, Arts and Sciences  Los Angeles CA USA"},{"name":"Biochemistry and Molecular Medicine Department USC Keck School of Medicine  Los Angeles CA USA"},{"name":"USC Norris Comprehensive Cancer Center Epigenetics and Gene Regulation  Los Angeles CA USA"},{"name":"USC Stem Cell Initiative  Los Angeles CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,5,19]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.26429"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12034"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells7080103"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2010.05.004"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellimm.2024.104825"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2014.11.018"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3073"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-12348-6"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2016.90"},{"key":"e_1_2_10_11_1","first-page":"198","article-title":"The influence of sex and gender on 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