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Previous studies demonstrated a type\u2010I interferon (IFN\u2010I) signature in skin DC2s following immunization with non\u2010viable larvae of the helminth <jats:italic>Nippostrongylus brasiliensis<\/jats:italic> (<jats:italic>Nb<\/jats:italic>), house dust mite (HDM) or <jats:italic>Schistosoma<\/jats:italic> egg antigen (SEA). Here we show that conditional loss of IFNAR1 signaling in CD11c<jats:sup>+<\/jats:sup> DCs significantly impaired T<jats:sub>H<\/jats:sub>2 effector and T follicular helper (TFH) CD4<jats:sup>+<\/jats:sup> T\u00a0cell responses to <jats:italic>Nb. In vivo<\/jats:italic> proliferation experiments demonstrated reduced numbers of highly divided CD4<jats:sup>+<\/jats:sup> T\u00a0cells in IFNAR1<jats:sup>\u2206CD11c<\/jats:sup> mice compared to IFNAR1<jats:sup>WT<\/jats:sup>, with similar proportions of GATA3<jats:sup>hi<\/jats:sup> T<jats:sub>H<\/jats:sub>2 cells within the divided populations indicating that IFNAR1 signaling in DCs was supporting T\u00a0cell priming and expansion rather than GATA3<jats:sup>hi<\/jats:sup> differentiation. By contrast, TFHs were present in lower frequencies in IFNAR1<jats:sup>\u2206CD11c<\/jats:sup> mice compared to IFNAR1<jats:sup>WT<\/jats:sup>, suggesting that IFN\u2010I signaling in DCs is necessary for allergen\u2010specific TFH differentiation. Characterization of the DC2 compartment by flow cytometry and bulk RNAseq demonstrated lower numbers of <jats:italic>Nb<\/jats:italic><jats:sup>+<\/jats:sup> DC2s in draining lymph nodes (dLN) and reduced expression of genes involved in DC2 motility, focal adhesion, and antigen processing, while expression of costimulatory molecules and cell survival and apoptosis pathway scores were similar. Therefore, IFN\u2010I conditioning of skin DC2s is necessary for their effective priming of CD4<jats:sup>+<\/jats:sup> T<jats:sub>H<\/jats:sub>2 responses to allergens and likely acts through the additive effects of multiple IFN\u2010I\u2010regulated pathways in DC2s.<\/jats:p>","DOI":"10.1111\/imcb.70035","type":"journal-article","created":{"date-parts":[[2025,6,4]],"date-time":"2025-06-04T00:40:47Z","timestamp":1748997647000},"page":"578-594","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Interferon signaling in type\u20102 dendritic cells supports <scp>T<sub>H<\/sub>2<\/scp> and T follicular helper fates in response to allergens"],"prefix":"10.1111","volume":"103","author":[{"given":"Greta R","family":"Webb","sequence":"first","affiliation":[{"name":"Malaghan Institute of Medical Research  Wellington New Zealand"},{"name":"University of Otago Wellington  Wellington New 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