{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T01:25:26Z","timestamp":1780363526291,"version":"3.54.1"},"reference-count":51,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,11,20]],"date-time":"2025-11-20T00:00:00Z","timestamp":1763596800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,11,20]],"date-time":"2025-11-20T00:00:00Z","timestamp":1763596800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunol Cell Biol"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Silicosis is a progressive occupational lung disease marked by persistent silica\u2010induced inflammation and irreversible pulmonary fibrosis. The NLRP3 inflammasome, an innate immune sensor, has been implicated as a key driver of silica\u2010triggered inflammation and fibrosis in preclinical models. However, the specific role of NLRP3 in immune cells, particularly within myeloid cells (monocytes, macrophages and neutrophils), remains poorly defined. In this study, we investigated the\n                    <jats:italic>in vivo<\/jats:italic>\n                    contribution of myeloid\u2010derived NLRP3 to silica\u2010induced lung pathology using a conditional NLRP3 knockout mouse model (\n                    <jats:italic>LysM<\/jats:italic>\n                    <jats:sup>Cre<\/jats:sup>\n                    <jats:italic>Nlrp<\/jats:italic>\n                    3\n                    <jats:sup>fl\/fl<\/jats:sup>\n                    ). These mice exhibited efficient deletion of NLRP3 in both resident and infiltrating lung myeloid cells. Following intranasal delivery of 2\u2009mg of silica, NLRP3 expression was upregulated in myeloid cells by day 3. Despite upregulation of NLRP3 in myeloid cells by day 3, early inflammasome activation in the tissue and BAL, including caspase\u20101 cleavage and IL\u20101\u03b2 and IL\u201018 secretion, remained intact. During the chronic phase (days 14 and 28), myeloid NLRP3 deletion did not mitigate hallmark features of silicosis, including alveolitis, structural lung damage, airway remodeling or peribronchial alpha\u2010smooth muscle actin expression. Furthermore, the formation and size of silicotic nodules were unaffected. These findings indicate that NLRP3 expression in myeloid cells is not essential for the development of silica\u2010induced pulmonary inflammation, tissue damage or fibrosis. This work highlights the need to explore alternative cellular sources and mechanisms of NLRP3\u2010driven pathology in silicosis.\n                  <\/jats:p>","DOI":"10.1111\/imcb.70067","type":"journal-article","created":{"date-parts":[[2025,11,20]],"date-time":"2025-11-20T22:45:01Z","timestamp":1763678701000},"page":"20-34","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Myeloid cell\u2010derived NLRP3 is dispensable for silica\u2010induced pulmonary inflammation and pathology"],"prefix":"10.1111","volume":"104","author":[{"given":"Kristian T","family":"Barry","sequence":"first","affiliation":[{"name":"Centre for Innate Immunity and Infectious Disease Hudson Institute of Medical Research  Clayton VIC Australia"},{"name":"Department of Molecular Translational Sciences Monash University  Clayton VIC Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher M","family":"Harpur","sequence":"additional","affiliation":[{"name":"Centre for Innate Immunity and Infectious Disease Hudson Institute of Medical Research  Clayton VIC Australia"},{"name":"Department of Molecular Translational Sciences Monash University  Clayton VIC Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca L","family":"Ambrose","sequence":"additional","affiliation":[{"name":"Centre for Innate Immunity and Infectious Disease Hudson Institute of Medical Research  Clayton VIC Australia"},{"name":"Department of Molecular Translational Sciences Monash University  Clayton VIC Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher J","family":"Hodges","sequence":"additional","affiliation":[{"name":"Centre for Innate Immunity and Infectious Disease Hudson Institute of Medical Research  Clayton VIC Australia"},{"name":"Department of Molecular Translational Sciences Monash University  Clayton VIC Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6540-1281","authenticated-orcid":false,"given":"Ashley","family":"Mansell","sequence":"additional","affiliation":[{"name":"Centre for Innate Immunity and Infectious Disease Hudson Institute of Medical Research  Clayton VIC Australia"},{"name":"Department of Molecular Translational Sciences Monash University  Clayton VIC Australia"},{"name":"Department of Microbiology, Anatomy, Physiology, and Pharmacology La Trobe University  Bundoora VIC Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3804-3298","authenticated-orcid":false,"given":"Maggie","family":"Lam","sequence":"additional","affiliation":[{"name":"Centre for Innate Immunity and Infectious Disease Hudson Institute of Medical Research  Clayton VIC Australia"},{"name":"Department of Molecular Translational Sciences Monash University  Clayton VIC Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0587-5514","authenticated-orcid":false,"given":"Michelle D","family":"Tate","sequence":"additional","affiliation":[{"name":"Centre for Innate Immunity and Infectious Disease Hudson Institute of Medical Research  Clayton VIC Australia"},{"name":"Department of Molecular Translational Sciences Monash University  Clayton VIC Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,20]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(12)60235-9"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1378\/chest.11-1321"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1136\/oemed-2017-104428"},{"key":"e_1_2_9_5_1","unstructured":"Safe Work Australia.Crystalline silica and silicosis.https:\/\/www.safeworkaustralia.gov.au\/safety\u2010topic\/hazards\/crystalline\u2010silica\u2010and\u2010silicosis. 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