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While peripheral blood immune profiles have been well\u2010defined, strain\u2010 and sex\u2010specific differences in leukocyte composition across other tissues remain poorly characterized. Here, we performed flow cytometric immunophenotyping of 19 leukocyte subsets and 3\u2009T cell memory phenotypes in the blood, bone marrow, liver, spleen, and lymph nodes of seven strains of na\u00efve adult male mice: Balb.b, BALB\/c, BALB\/c Ly5.1, C57BL\/6J, C57BL\/6J Ly5.1, BALB\/c:B6J (F1), and DBA\/2\u2009J. Parallel analyses were conducted in female BALB\/c and C57BL\/6J mice. Each tissue contained a unique immunobiome, with conventional T and B cells enriched in the lymph nodes and spleen, unconventional T cells and innate lymphoid cells (ILCs) in the liver, and myeloid cells in the bone marrow. Generally, BALB\u2010derived strains displayed a helper T cell\u2010 and innate\u2010skewed profile, enriched for CD4 and regulatory T cells, neutrophils, and natural killer cells. C57BL\/6J\u2010derived strains were typically enriched for CD8 T cells, ILC1s, and conventional dendritic cells, consistent with a cytotoxic immune bias. Small differences were detected between congenic and parental strains. F1's exhibited a mix of C57BL\/6J\u2010biased and intermediate parental phenotypes. DBA\/2\u2009J mice were immunologically distinct, notably enriched for \u03b3\u03b4 T cells. Sex\u2010associated differences were minor compared to strain effects. These findings provide tissue\u2010specific benchmarks for leukocyte composition across commonly used mouse strains. They also highlight the importance of strain and sex selection and baseline immunophenotyping to support accurate interpretation of observed immune phenotypes in experimental contexts.\n                  <\/jats:p>","DOI":"10.1111\/imcb.70168","type":"journal-article","created":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T09:21:24Z","timestamp":1791451284000},"page":"1019-1034","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The Leukocyte Landscape Across Mouse Strains and Tissues"],"prefix":"10.1111","volume":"104","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-7007-8944","authenticated-orcid":false,"given":"Ella","family":"Trolio","sequence":"first","affiliation":[{"name":"Medical School, University of Western Australia  Perth 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