{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,23]],"date-time":"2023-11-23T00:07:27Z","timestamp":1700698047193},"reference-count":37,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,6,13]],"date-time":"2013-06-13T00:00:00Z","timestamp":1371081600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunology"],"published-print":{"date-parts":[[2013,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content>\u2010specific T cells that produce interferon\u2010\u03b3 (<jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>\u2010\u03b3) are present in the genital tract of <jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content>\u2010infected women although these do not provide protection against genital <jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content> shedding. Because polyfunctional <jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content>\u2010specific <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content> cells have been implicated in better <jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content> control than those with a single function, this study aimed to investigate whether polyfunctional <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content> cells were present at the female genital mucosa. Cervical cytobrush\u2010derived <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content> cells were obtained from chronically <jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content>\u2010infected women and compared with blood. <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>3<jats:sup>+<\/jats:sup> T cells from both compartments were expanded with Dynal anti\u2010<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>3\/<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>28 expander beads for 14\u00a0days and flow cytometry was used to evaluate four <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\u2010cell functions (<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>107a, <jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content><jats:italic>\u2010<\/jats:italic>\u03b3, tumour necrosis factor\u2010\u03b1 and macrophage inflammatory protein\u20101\u03b2) from 16 women. The majority of Gag\u2010specific T\u2010cell responses in the female genital tract were monofunctional, although low frequencies of <jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content> Gag\u2010specific polyfunctional <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>8<jats:sup>+<\/jats:sup> <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content> cells were detected at the cervix in 81\u00b73% (13\/16) of women. The ability of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>8<jats:sup>+<\/jats:sup> <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content> cells at both the cervix and in blood to express <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>107a and to exhibit polyfunctional responses (two or more functions) following Gag stimulation was inversely associated with plasma viral load and positively associated with blood <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4 counts, suggesting that clinical status impacted on the functionality of <jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content>\u2010specific <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content> cells at the mucosa, in a similar way to blood. <jats:styled-content style=\"fixed-case\">HIV G<\/jats:styled-content>ag\u2010specific cervical <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content> cells were largely monofunctional. Polyfunctional <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content> cells were detected at the cervix in women with high blood <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4 count and low plasma viral load but these did not protect from <jats:styled-content style=\"fixed-case\">HIV<\/jats:styled-content> genital shedding.<\/jats:p>","DOI":"10.1111\/imm.12084","type":"journal-article","created":{"date-parts":[[2013,2,1]],"date-time":"2013-02-01T20:12:16Z","timestamp":1359749536000},"page":"342-351","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["<scp>HIV<\/scp>\u2010specific <scp>T<\/scp>\u2010cell responses detected in the genital tract of chronically <scp>HIV<\/scp>\u2010infected women are largely monofunctional"],"prefix":"10.1111","volume":"139","author":[{"given":"Alfred","family":"Bere","sequence":"first","affiliation":[{"name":"Division of Medical Virology Faculty of 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