{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T09:16:07Z","timestamp":1776503767550,"version":"3.51.2"},"reference-count":46,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,6,13]],"date-time":"2013-06-13T00:00:00Z","timestamp":1371081600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunology"],"published-print":{"date-parts":[[2013,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Docosahexaenoic acid (<jats:styled-content style=\"fixed-case\">DHA<\/jats:styled-content>) and eicosapentaenoic acid (<jats:styled-content style=\"fixed-case\">EPA<\/jats:styled-content>) are bioactive n\u20103 long\u2010chain polyunsaturated fatty acids (<jats:styled-content style=\"fixed-case\">LCPUFA<\/jats:styled-content>s) in fish oil that exert immunosuppressive effects. A significant amount of literature shows that n\u20103 <jats:styled-content style=\"fixed-case\">LCPUFA<\/jats:styled-content>s suppress dendritic cell (<jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>) function <jats:italic>in vitro<\/jats:italic>; however, few studies have determined if the effects are emulated at the animal level. In this study, we first focused on the functional consequences of 5% (weight\/weight) fish oil on splenic <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>11c<jats:sup>+<\/jats:sup> <jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>s. Administration of n\u20103 <jats:styled-content style=\"fixed-case\">LCPUFA<\/jats:styled-content>s, modelling human pharmacological intake (2% of total kcal from <jats:styled-content style=\"fixed-case\">EPA<\/jats:styled-content>,1\u00b73% from <jats:styled-content style=\"fixed-case\">DHA<\/jats:styled-content>), to C57<jats:styled-content style=\"fixed-case\">BL<\/jats:styled-content>\/6 mice for 3\u00a0weeks reduced <jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content> surface expression of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>80 by 14% and tumour necrosis factor\u2010\u03b1 secretion by 29% upon lipopolysaccharide stimulation relative to a control diet. The n\u20103 <jats:styled-content style=\"fixed-case\">LCPUFA<\/jats:styled-content>s also significantly decreased <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>11c<jats:sup>+<\/jats:sup> surface expression and phagocytosis by 12% compared with the control diet. Antigen presentation studies revealed a 22% decrease in <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>69 surface expression on transgenic <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4<jats:sup>+<\/jats:sup> T lymphocytes activated by <jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>s from mice fed fish oil. We then determined if the functional changes were mechanistically associated with changes in lipid microdomain clustering or plasma membrane microviscosity with n\u20103 <jats:styled-content style=\"fixed-case\">LCPUFA<\/jats:styled-content>s, as reported for B and T lymphocytes. Fish oil administration to mice did not influence cholera\u2010toxin induced lipid microdomain clustering or microviscosity, even though <jats:styled-content style=\"fixed-case\">EPA<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">DHA<\/jats:styled-content> levels were significantly elevated relative to the control diet. Overall, our data show that n\u20103 <jats:styled-content style=\"fixed-case\">LCPUFA<\/jats:styled-content>s exert immunosuppressive effects on <jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content>s, validating <jats:italic>in vitro<\/jats:italic> studies. The results also show that <jats:styled-content style=\"fixed-case\">DC<\/jats:styled-content> microdomain clustering and microviscosity were not changed by the n\u20103 <jats:styled-content style=\"fixed-case\">LCPUFA<\/jats:styled-content> intervention used in this study.<\/jats:p>","DOI":"10.1111\/imm.12088","type":"journal-article","created":{"date-parts":[[2013,2,4]],"date-time":"2013-02-04T15:49:23Z","timestamp":1359992963000},"page":"386-394","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Dendritic cell activation, phagocytosis and <scp>CD<\/scp>69 expression on cognate T cells are suppressed by n\u20103 long\u2010chain polyunsaturated fatty acids"],"prefix":"10.1111","volume":"139","author":[{"given":"Heather","family":"Teague","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology East Carolina University Greenville NC USA"},{"name":"East Carolina Diabetes and Obesity 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