{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T05:30:07Z","timestamp":1790746207810,"version":"4.1.0"},"reference-count":49,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,7,2]],"date-time":"2013-07-02T00:00:00Z","timestamp":1372723200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunology"],"published-print":{"date-parts":[[2013,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>chistosomiasis japonica is a severe tropical disease caused by the parasitic worm <jats:italic><jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>chistosoma japonicum<\/jats:italic>. Among the most serious pathological effects of <jats:italic><jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>.\u00a0japonicum<\/jats:italic> infection are hepatic lesions (cirrhosis and fibrosis) and portal hypertension. <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>nterleukin\u201017 (<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201017) is a pro\u2010inflammatory cytokine involved in the pathogenesis of many inflammatory and infectious conditions, including schistosomiasis. We infected C57BL\/6 mice with <jats:italic><jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>.\u00a0japonicum<\/jats:italic> and isolated lymphocytes from the liver to identify cell subsets with high <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201017 expression and release using flow cytometry and <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>. Expression and release of IL\u201017 was significantly higher in hepatic lymphocytes from infected mice compared with control mice in response to both non\u2010specific stimulation with anti\u2010CD3 monoclonal antibody plus\/anti\u2010CD28 monoclonal antibody and PMA plus ionomycin. We then compared <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201017 expression in three hepatic T\u2010cell subsets, T helper, natural killer <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content> and \u03b3\u03b4T cells, to determine the major source of <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201017 during infection. Interleukin\u201017 was induced in all three subsets by PMA\u00a0+\u00a0ionomycin, but \u03b3\u03b4T lymphocytes exhibited the largest increase in expression. We then established a mouse model to further investigate the role of <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201017 in granulomatous and fibrosing inflammation against parasite eggs. Reducing <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201017 activity using anti\u2010<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201017A antibodies decreased infiltration of inflammatory cells and collagen deposition in the livers of infected C57BL\/6 mice. The serum levels of soluble egg antigen (<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>) \u2010specific IgGs were enhanced by anti\u2010IL\u201017A monoclonal antibody blockade, suggesting that <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201017 normally serves to suppress this humoral response. These findings suggest that \u03b3\u03b4T cells are the most <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201017\u2010producing cells and that IL\u201017 contributes to granulomatous inflammatory and fibrosing reactions in <jats:italic><jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>.\u00a0japonicum\u2010<\/jats:italic>infected C57BL\/6 mouse liver.<\/jats:p>","DOI":"10.1111\/imm.12105","type":"journal-article","created":{"date-parts":[[2013,4,2]],"date-time":"2013-04-02T14:22:03Z","timestamp":1364912523000},"page":"523-532","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":74,"title":["Characteristics of <scp>IL<\/scp>\u201017 induction by <i><scp>S<\/scp>chistosoma japonicum<\/i> infection in C57<scp>BL<\/scp>\/6 mouse liver"],"prefix":"10.1111","volume":"139","author":[{"given":"Dianhui","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Pathogenic Biology and 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