{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T21:39:31Z","timestamp":1768513171404,"version":"3.49.0"},"reference-count":45,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,12,10]],"date-time":"2013-12-10T00:00:00Z","timestamp":1386633600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100010089","name":"JGW Patterson Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100010089","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Newcastle Healthcare Charity and Newcastle upon Tyne Hospitals NHS Charity"},{"name":"Arthritis Research UK PhD studentship"},{"name":"Newcastle University PhD studentship"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunology"],"published-print":{"date-parts":[[2014,1]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>The majority of studies examining antigen\u2010presenting cell (<jats:styled-content style=\"fixed-case\">APC<\/jats:styled-content>) function have focused on the capture and presentation of antigens released from pathogens or damaged cells. However, antigen\u2010specific <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content> cells are also capable of efficiently extracting antigens that are either tethered to, or integrally part of the plasma membrane of various target cells. In this study we show that <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content> cells are also highly efficient at extracting integral components of the extracellular matrix (<jats:styled-content style=\"fixed-case\">ECM<\/jats:styled-content>) for subsequent presentation. In particular we demonstrate that <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content> cells specific for aggrecan, an integral component of cartilage <jats:styled-content style=\"fixed-case\">ECM<\/jats:styled-content>, acquire this rheumatoid arthritis candidate autoantigen in both a <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content>\u2010cell\u2010receptor\u2010dependent and a contact\u2010dependent manner. We also demonstrate that the subsequent presentation of aggregan from <jats:styled-content style=\"fixed-case\">ECM<\/jats:styled-content> leads to <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4<jats:sup>+<\/jats:sup> <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\u2010cell activation and effector cell formation. Recent studies have identified B\u2010cell\u2010mediated antigen presentation as essential for the development of autoimmunity, but a unique role for B cells compared with other <jats:styled-content style=\"fixed-case\">APC<\/jats:styled-content> has yet to be defined. Our findings lead us to propose that the acquisition of <jats:styled-content style=\"fixed-case\">ECM<\/jats:styled-content>\u2010derived autoantigens represents a mechanism that defines the <jats:styled-content style=\"fixed-case\">APC<\/jats:styled-content> requirement for B cells in the development of autoimmunity.<\/jats:p>","DOI":"10.1111\/imm.12169","type":"journal-article","created":{"date-parts":[[2013,9,11]],"date-time":"2013-09-11T13:13:52Z","timestamp":1378905232000},"page":"70-78","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Antigen\u2010specific <scp>B<\/scp> lymphocytes acquire proteoglycan aggrecan from cartilage extracellular matrix resulting in antigen presentation and <scp>CD<\/scp>4<sup>+<\/sup><scp>T<\/scp>\u2010cell activation"],"prefix":"10.1111","volume":"141","author":[{"given":"Marzena","family":"Ciechomska","sequence":"first","affiliation":[{"name":"Institute of Cellular Medicine Faculty of Medical Sciences Newcastle University Newcastle upon Tyne UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caroline L.","family":"Wilson","sequence":"additional","affiliation":[{"name":"Institute of Cellular Medicine Faculty of Medical Sciences Newcastle University Newcastle upon Tyne UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Achilleas","family":"Floudas","sequence":"additional","affiliation":[{"name":"Institute of Cellular Medicine Faculty of Medical Sciences Newcastle University Newcastle upon Tyne UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wang","family":"Hui","sequence":"additional","affiliation":[{"name":"Institute of Cellular Medicine Faculty of Medical Sciences Newcastle University Newcastle upon Tyne UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew D.","family":"Rowan","sequence":"additional","affiliation":[{"name":"Institute of Cellular Medicine Faculty of Medical Sciences Newcastle University Newcastle upon Tyne UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Willem","family":"van Eden","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Immunology Faculty of Veterinary Medicine University of Utrecht Utrecht the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John H.","family":"Robinson","sequence":"additional","affiliation":[{"name":"Institute of Cellular Medicine Faculty of Medical Sciences Newcastle University Newcastle upon Tyne UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew M.","family":"Knight","sequence":"additional","affiliation":[{"name":"Institute of Cellular Medicine Faculty of Medical Sciences Newcastle University Newcastle upon Tyne UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,12,10]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.127.5.1869"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0105-2896.2009.00884.x"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.160.4.1102"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/314537a0"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.183.3.891"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.189.10.1639"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.53.10.2581"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.174.6.3781"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.176.6.3498"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0803706"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90473-4"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/16.13.3842"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/35078099"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201141472"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.173.6.1433"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.160.1.51"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.167.8.4710"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.7.4481"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0806874105"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/kes113"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar2125"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymeth.2008.01.009"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.44.28057"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780360906"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI118458"},{"key":"e_1_2_8_27_1","doi-asserted-by":"crossref","first-page":"3812","DOI":"10.4049\/jimmunol.160.8.3812","article-title":"Critical roles of glycosaminoglycan side chains of cartilage proteoglycan (aggrecan) in antigen recognition and presentation","volume":"160","author":"Glant TT","year":"1998","journal-title":"J Immunol"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2567.2011.03548.x"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.122.2.549"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaut.2005.09.017"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/268154a0"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.2000.01174.x"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.181.1.329"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1995.2476"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.179.8.5109"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/19.4.513"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1123940"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20101125"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(04)00105-0"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7600944"},{"key":"e_1_2_8_41_1","doi-asserted-by":"crossref","first-page":"1424","DOI":"10.4049\/jimmunol.149.4.1424","article-title":"\u03b14\/\u03b21 integrin (VLA\u20104) ligands in arthritis. Vascular cell adhesion molecule\u20101 expression in synovium and on fibroblast\u2010like synoviocytes","volume":"149","author":"Morales\u2010Ducret J","year":"1992","journal-title":"J Immunol"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1237572"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2011.07.008"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardiores.2005.12.002"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.2741\/2080"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellimm.2004.08.006"}],"container-title":["Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fimm.12169","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/imm.12169","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T03:13:40Z","timestamp":1696216420000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/imm.12169"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,12,10]]},"references-count":45,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2014,1]]}},"alternative-id":["10.1111\/imm.12169"],"URL":"https:\/\/doi.org\/10.1111\/imm.12169","archive":["Portico"],"relation":{},"ISSN":["0019-2805","1365-2567"],"issn-type":[{"value":"0019-2805","type":"print"},{"value":"1365-2567","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,12,10]]},"assertion":[{"value":"2013-08-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-09-03","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-12-10","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}