{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T10:30:25Z","timestamp":1762079425876},"reference-count":29,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2014,4,24]],"date-time":"2014-04-24T00:00:00Z","timestamp":1398297600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/3.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunology"],"published-print":{"date-parts":[[2014,6]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Children with systemic <jats:styled-content style=\"fixed-case\">J<\/jats:styled-content>uvenile <jats:styled-content style=\"fixed-case\">I<\/jats:styled-content>diopathic <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>rthritis (s<jats:styled-content style=\"fixed-case\">JIA<\/jats:styled-content>), the most severe subtype of <jats:styled-content style=\"fixed-case\">JIA<\/jats:styled-content>, are at risk from destructive polyarthritis and growth failure, and corticosteroids as part of conventional treatment can result in osteoporosis and growth delay. In children where there is failure or toxicity from drug therapies, disease has been successfully controlled by <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\u2010cell\u2010depleted autologous stem cell transplantation (<jats:styled-content style=\"fixed-case\">ASCT<\/jats:styled-content>). At present, the immunological basis underlying remission after <jats:styled-content style=\"fixed-case\">ASCT<\/jats:styled-content> is unknown. Immune reconstitution of <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content> cells, <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content> cells, natural killer cells, natural killer <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content> cells and monocytes, in parallel with <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\u2010cell receptor (<jats:styled-content style=\"fixed-case\">TCR<\/jats:styled-content>) diversity by analysis of the \u03b2 variable region (<jats:styled-content style=\"fixed-case\">TCRV<\/jats:styled-content>b) complementarity determining region\u20103 (<jats:styled-content style=\"fixed-case\">CDR<\/jats:styled-content>3) using spectratyping and sequencing, were studied in five children with s<jats:styled-content style=\"fixed-case\">JIA<\/jats:styled-content> before and after <jats:styled-content style=\"fixed-case\">ASCT<\/jats:styled-content>. At time of follow up (mean 11\u00b75\u00a0years), four patients remain in complete remission, while one child relapsed within 1\u00a0month of transplant. The <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>8<jats:sup>+<\/jats:sup> <jats:styled-content style=\"fixed-case\">TCRV<\/jats:styled-content>b repertoire was highly oligoclonal early in immune reconstitution and re\u2010emergence of pre\u2010transplant <jats:styled-content style=\"fixed-case\">TCRV<\/jats:styled-content>b <jats:styled-content style=\"fixed-case\">CDR<\/jats:styled-content>3 dominant peaks was observed after transplant in certain <jats:styled-content style=\"fixed-case\">TCRV<\/jats:styled-content>b families. Further, re\u2010emergence of pre\u2010<jats:styled-content style=\"fixed-case\">ASCT<\/jats:styled-content> clonal sequences in addition to new sequences was identified after transplant. These results suggest that a chimeric <jats:styled-content style=\"fixed-case\">TCR<\/jats:styled-content> repertoire, comprising <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content>\u2010cell clones developed before and after transplant, can be associated with clinical remission from severe arthritis.<\/jats:p>","DOI":"10.1111\/imm.12245","type":"journal-article","created":{"date-parts":[[2014,1,10]],"date-time":"2014-01-10T13:19:08Z","timestamp":1389359948000},"page":"227-236","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Immunological characteristics and <scp>T<\/scp>\u2010cell receptor clonal diversity in children with systemic juvenile idiopathic arthritis undergoing <scp>T<\/scp>\u2010cell\u2010depleted autologous stem cell transplantation"],"prefix":"10.1111","volume":"142","author":[{"given":"Qiong","family":"Wu","sequence":"first","affiliation":[{"name":"Rheumatology Unit UCL Institute of Child Health University College London London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne M.","family":"Pesenacker","sequence":"additional","affiliation":[{"name":"Rheumatology Unit UCL Institute of Child Health University College London London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alka","family":"Stansfield","sequence":"additional","affiliation":[{"name":"Scientific Research Laboratory Cell Medica London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Douglas","family":"King","sequence":"additional","affiliation":[{"name":"Division of Infection and Immunity Department of Immunology University College London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dawn","family":"Barge","sequence":"additional","affiliation":[{"name":"Department of Paediatric Immunology Great North Children's Hospital Newcastle upon Tyne Hospitals NHS Foundation Trust Newcastle upon Tyne UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helen E.","family":"Foster","sequence":"additional","affiliation":[{"name":"Musculoskeletal Research Group Institute of Cellular Medicine Newcastle University, and Great North Children's Hospital London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mario","family":"Abinun","sequence":"additional","affiliation":[{"name":"Department of Paediatric Immunology Great North Children's Hospital Newcastle upon Tyne Hospitals NHS Foundation Trust Newcastle upon Tyne UK"},{"name":"Musculoskeletal Research Group Institute of Cellular Medicine Newcastle University, and Great North Children's Hospital London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucy R.","family":"Wedderburn","sequence":"additional","affiliation":[{"name":"Rheumatology Unit UCL Institute of Child Health University College London London UK"},{"name":"Arthritis Research UK Centre for Adolescent Rheumatology University College London UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,4,24]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"390","article-title":"International League of Associations for Rheumatology classification of juvenile idiopathic arthritis: second revision, Edmonton, 2001","volume":"31","author":"Petty RE","year":"2004","journal-title":"J Rheumatol"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(07)60363-8"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2011.68"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/41.12.1428"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2008.12.029"},{"key":"e_1_2_8_7_1","doi-asserted-by":"crossref","first-page":"1696","DOI":"10.1182\/blood-2005-07-2800","article-title":"Autologous stem cell transplantation for autoimmunity induces immunologic self\u2010tolerance by reprogramming autoreactive T cells and restoring the CD4+\u00a0CD25+ immune regulatory network","volume":"107","author":"Kleer I","year":"2006","journal-title":"Blood"},{"key":"e_1_2_8_8_1","doi-asserted-by":"crossref","first-page":"1318","DOI":"10.1136\/ard.2003.017798","article-title":"Autologous stem cell transplantation for refractory juvenile idiopathic arthritis: analysis of clinical effects, mortality, and transplant related morbidity","volume":"63","author":"Kleer IM","year":"2004","journal-title":"Ann Rheum Dis"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(98)05399-9"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1703946"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.22656"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00415-002-0800-7"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(199911)42:11<2281::AID-ANR4>3.0.CO;2-E"},{"key":"e_1_2_8_14_1","first-page":"25","article-title":"Intensive immunosuppression and autologous stem cell transplantation for patients with severe rheumatoid arthritis: the Leiden experience","volume":"64","author":"Laar JM","year":"2001","journal-title":"J Rheumatol Suppl"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.transproceed.2011.03.039"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1136\/adc.88.3.201"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature03728"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20041679"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1159\/000117824"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.23784"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V91.7.2588"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/40.11.1299"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1700624"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.9.4319"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.152.10.5109"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.11174"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780391108"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri1593"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V89.10.3700"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-12-128488"}],"container-title":["Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fimm.12245","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/imm.12245","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T08:02:02Z","timestamp":1696233722000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/imm.12245"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,4,24]]},"references-count":29,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2014,6]]}},"alternative-id":["10.1111\/imm.12245"],"URL":"https:\/\/doi.org\/10.1111\/imm.12245","archive":["Portico"],"relation":{},"ISSN":["0019-2805","1365-2567"],"issn-type":[{"value":"0019-2805","type":"print"},{"value":"1365-2567","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,4,24]]},"assertion":[{"value":"2013-08-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-01-05","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2014-04-24","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}