{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T07:04:51Z","timestamp":1761807891066,"version":"3.37.3"},"reference-count":49,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,7,29]],"date-time":"2014-07-29T00:00:00Z","timestamp":1406592000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/J004332\/1"],"award-info":[{"award-number":["BB\/J004332\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/F019726\/1"],"award-info":[{"award-number":["BB\/F019726\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunology"],"published-print":{"date-parts":[[2014,9]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Prions are a unique group of pathogens, which are considered to comprise solely of an abnormally folded isoform of the cellular prion protein. The accumulation and replication of prions within secondary lymphoid organs is important for their efficient spread from the periphery to the brain where they ultimately cause neurodegeneration and death. Mononuclear phagocytes (<jats:styled-content style=\"fixed-case\">MNP<\/jats:styled-content>) play key roles in prion disease pathogenesis. Some <jats:styled-content style=\"fixed-case\">MNP<\/jats:styled-content> appear to facilitate the propagation of prions to and within lymphoid tissues, whereas others may aid their clearance by phagocytosis and by destroying them. Our recent data show that an intact splenic marginal zone is important for the efficient delivery of prions into the B\u2010cell follicles where they subsequently replicate upon follicular dendritic cells before infecting the nervous system. Sialoadhesin is an <jats:styled-content style=\"fixed-case\">MNP<\/jats:styled-content>\u2010restricted cell adhesion molecule that binds sialylated glycoproteins. Sialoadhesin is constitutively expressed upon splenic marginal zone metallophilic and lymph node sub\u2010capsular sinus macrophage populations, where it may function to bind sialylated glycoproteins, pathogens and exosomes in the blood and lymph via recognition of terminal sialic acid residues. As the prion glycoprotein is highly sialylated, we tested the hypothesis that sialoadhesin may influence prion disease pathogenesis. We show that after peripheral exposure, prion pathogenesis was unaltered in sialoadhesin\u2010deficient mice; revealing that lymphoid sequestration of prions is not mediated via sialoadhesin. Hence, although an intact marginal zone is important for the efficient uptake and delivery of prions into the B\u2010cell follicles of the spleen, this is not influenced by sialoadhesin expression by the <jats:styled-content style=\"fixed-case\">MNP<\/jats:styled-content> within it.<\/jats:p>","DOI":"10.1111\/imm.12294","type":"journal-article","created":{"date-parts":[[2014,3,29]],"date-time":"2014-03-29T03:44:48Z","timestamp":1396064688000},"page":"120-129","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Peripheral prion disease pathogenesis is unaltered in the absence of sialoadhesin (Siglec\u20101\/<scp>CD<\/scp>169)"],"prefix":"10.1111","volume":"143","author":[{"given":"Barry M.","family":"Bradford","sequence":"first","affiliation":[{"name":"The Roslin Institute and R(D)SVS University of Edinburgh Midlothian UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul R.","family":"Crocker","sequence":"additional","affiliation":[{"name":"College of Life Sciences University of Dundee Dundee UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neil A.","family":"Mabbott","sequence":"additional","affiliation":[{"name":"The Roslin Institute and R(D)SVS University of Edinburgh Midlothian 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