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Previous studies reported that wogonin, a flavonoid\u2010like chemical compound which was found in<jats:italic>Scutellaria baicalensis<\/jats:italic>, has anti\u2010inflammatory effects in several inflammation models, but not in<jats:styled-content style=\"fixed-case\">ALI<\/jats:styled-content>. Here, the<jats:italic>in vivo<\/jats:italic>protective effect of wogonin in the amelioration of lipopolysaccharide (<jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content>) \u2010induced lung injury and inflammation was assessed. In addition, the<jats:italic>in vitro<\/jats:italic>effects and mechanisms of wogonin were studied in the mouse macrophage cell lines Ana\u20101 and<jats:styled-content style=\"fixed-case\">RAW<\/jats:styled-content>264.7.<jats:italic>In vivo<\/jats:italic>results indicated that wogonin attenuated<jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content>\u2010induced histological alterations. Peripheral blood leucocytes decreased in the<jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content>\u2010induced group, which was ameliorated by wogonin. In addition, wogonin inhibited the production of several inflammatory cytokines, including tumour necrosis factor\u2010<jats:italic>\u03b1<\/jats:italic>, interleukin\u20101<jats:italic>\u03b2<\/jats:italic>(<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20101<jats:italic>\u03b2<\/jats:italic>) and<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20106, in the bronchoalveolar lavage fluid and lung tissues after<jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content>challenge, while the peroxisome proliferator\u2010activated receptor<jats:italic>\u03b3<\/jats:italic>(<jats:styled-content style=\"fixed-case\">PPAR<\/jats:styled-content><jats:italic>\u03b3<\/jats:italic>) inhibitor<jats:styled-content style=\"fixed-case\">GW<\/jats:styled-content>9662 reversed these effects.<jats:italic>In vitro<\/jats:italic>results indicated that wogonin significantly decreased the secretion of<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20106,<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20101<jats:italic>\u03b2<\/jats:italic>and tumour necrosis factor\u2010<jats:italic>\u03b1<\/jats:italic>in Ana\u20101 and<jats:styled-content style=\"fixed-case\">RAW<\/jats:styled-content>264.7 cells, which was suppressed by transfection of<jats:styled-content style=\"fixed-case\">PPAR<\/jats:styled-content><jats:italic>\u03b3<\/jats:italic>small interfering<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>and<jats:styled-content style=\"fixed-case\">GW<\/jats:styled-content>9662 treatment. Moreover, wogonin activated<jats:styled-content style=\"fixed-case\">PPAR<\/jats:styled-content><jats:italic>\u03b3<\/jats:italic>, induced<jats:styled-content style=\"fixed-case\">PPAR<\/jats:styled-content><jats:italic>\u03b3<\/jats:italic>\u2010mediated attenuation of the nuclear translocation and the<jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\u2010binding activity of nuclear factor\u2010<jats:italic>\u03ba<\/jats:italic>B<jats:italic>in vivo<\/jats:italic>and<jats:italic>in vitro<\/jats:italic>. In conclusion, all of these results showed that wogonin may serve as a promising agent for the attenuation of<jats:styled-content style=\"fixed-case\">ALI<\/jats:styled-content>\u2010associated inflammation and pathology by regulating the<jats:styled-content style=\"fixed-case\">PPAR<\/jats:styled-content><jats:italic>\u03b3<\/jats:italic>\u2010involved nuclear factor\u2010<jats:italic>\u03ba<\/jats:italic>B pathway.<\/jats:p>","DOI":"10.1111\/imm.12305","type":"journal-article","created":{"date-parts":[[2014,4,25]],"date-time":"2014-04-25T17:24:28Z","timestamp":1398446668000},"page":"241-257","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":82,"title":["Wogonin prevents lipopolysaccharide\u2010induced acute lung injury and inflammation in mice via peroxisome proliferator\u2010activated receptor gamma\u2010mediated attenuation of the nuclear factor\u2010kappaB pathway"],"prefix":"10.1111","volume":"143","author":[{"given":"Jing","family":"Yao","sequence":"first","affiliation":[{"name":"Jiangsu Key Laboratory of Carcinogenesis and Intervention China Pharmaceutical University Nanjing China"},{"name":"School of Life Science and Technology China Pharmaceutical University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Di","family":"Pan","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Carcinogenesis and Intervention China Pharmaceutical University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Zhao","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Carcinogenesis and Intervention China Pharmaceutical University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Zhao","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Carcinogenesis and Intervention China 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