{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T16:48:12Z","timestamp":1761238092803},"reference-count":37,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2014,9,8]],"date-time":"2014-09-08T00:00:00Z","timestamp":1410134400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunology"],"published-print":{"date-parts":[[2014,10]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>To prevent the global spread of tuberculosis (<jats:styled-content style=\"fixed-case\">TB<\/jats:styled-content>), more effective vaccines and vaccination strategies are urgently needed. As a result of the success of bacillus Calmette\u2013Gu\u00e9rin (<jats:styled-content style=\"fixed-case\">BCG<\/jats:styled-content>) in protecting children against miliary and meningeal <jats:styled-content style=\"fixed-case\">TB<\/jats:styled-content>, the majority of individuals will have been vaccinated with <jats:styled-content style=\"fixed-case\">BCG<\/jats:styled-content>; hence, boosting <jats:styled-content style=\"fixed-case\">BCG<\/jats:styled-content>\u2010primed immunity will probably be a key component of future vaccine strategies. In this study, we compared the ability of <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\u2010, protein\u2010 and lentiviral vector\u2010based vaccines that express the antigens Ag85B and Rv3425 to boost the effects of <jats:styled-content style=\"fixed-case\">BCG<\/jats:styled-content> in the context of immunity and protection against <jats:italic>Mycobacterium tuberculosis<\/jats:italic> in C57<jats:styled-content style=\"fixed-case\">BL<\/jats:styled-content>\/6 mice. Our results demonstrated that prime\u2013boost <jats:styled-content style=\"fixed-case\">BCG<\/jats:styled-content> vaccination with a lentiviral vector expressing the antigens Ag85B and Rv3425 significantly enhanced immune responses, including T helper type 1 and <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>8<jats:sup>+<\/jats:sup> cytotoxic <jats:styled-content style=\"fixed-case\">T<\/jats:styled-content> lymphocyte responses, compared with <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\u2010 and protein\u2010based vaccines. However, lentivirus\u2010vectored and <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content>\u2010based vaccines greatly improved the protective efficacy of <jats:styled-content style=\"fixed-case\">BCG<\/jats:styled-content> against <jats:italic>M.\u00a0tuberculosis<\/jats:italic>, as indicated by a lack of weight loss and significantly reduced bacterial loads and histological damage in the lung. Our study suggests that the use of lentiviral or <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> vaccines containing the antigens Ag85B and Rv3425 to boost <jats:styled-content style=\"fixed-case\">BCG<\/jats:styled-content> is a good choice for the rational design of an efficient vaccination strategy against <jats:styled-content style=\"fixed-case\">TB<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/imm.12308","type":"journal-article","created":{"date-parts":[[2014,4,28]],"date-time":"2014-04-28T15:25:59Z","timestamp":1398698759000},"page":"277-286","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Prime\u2013boost bacillus Calmette\u2013Gu\u00e9rin vaccination with lentivirus\u2010vectored and <scp>DNA<\/scp>\u2010based vaccines expressing antigens Ag85B and Rv3425 improves protective efficacy against <i>Mycobacterium tuberculosis<\/i> in mice"],"prefix":"10.1111","volume":"143","author":[{"given":"Ying","family":"Xu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Genetic Engineering School of Life Science Fudan University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Enzhuo","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Genetic Engineering School of Life Science Fudan University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianguang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Orthopaedic Surgery Shanghai Tenth People's Hospital Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Genetic Engineering School of Life Science Fudan University Shanghai 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