{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T05:21:04Z","timestamp":1778563264213,"version":"3.51.4"},"reference-count":42,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2014,11,10]],"date-time":"2014-11-10T00:00:00Z","timestamp":1415577600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"National Health and Medical Research Council (NHMRC)","award":["553055"],"award-info":[{"award-number":["553055"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunology"],"published-print":{"date-parts":[[2014,12]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Immunoglobulin A is an important mucosal antibody that can neutralize mucosal pathogens by either preventing attachment to epithelia (immune exclusion) or alternatively inhibit intra\u2010epithelial replication following transcytosis by the polymeric immunoglobulin receptor (pIgR). <jats:italic><jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>hlamydia trachomatis<\/jats:italic> is a major human pathogen that initially targets the endocervical or urethral epithelium in women and men, respectively. As both tissues contain abundant secretory IgA (SIgA) we assessed the protection afforded by IgA targeting different chlamydial antigens expressed during the extra\u2010 and intra\u2010epithelial stages of infection. We developed an <jats:italic>in vitro<\/jats:italic> model using polarizing cells expressing the murine pIgR together with antigen\u2010specific mouse IgA, and an <jats:italic>in vivo<\/jats:italic> model using pIgR<jats:sup>\u2212\/\u2212<\/jats:sup> mice. Secretory IgA targeting the extra\u2010epithelial chlamydial antigen, the major outer membrane protein, significantly reduced infection <jats:italic>in vitro<\/jats:italic> by 24% and <jats:italic>in vivo<\/jats:italic> by 44%. Conversely, pIgR\u2010mediated delivery of IgA targeting the intra\u2010epithelial inclusion membrane protein A bound to the inclusion but did not reduce infection <jats:italic>in vitro<\/jats:italic> or <jats:italic>in vivo<\/jats:italic>. Similarly, intra\u2010epithelial IgA targeting the secreted protease <jats:italic><jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>hlamydia<\/jats:italic> protease\u2010like activity factor also failed to reduce infection. Together, these data suggest the importance of pIgR\u2010mediated delivery of IgA targeting extra\u2010epithelial, but not intra\u2010epithelial, chlamydial antigens for protection against a genital tract infection.<\/jats:p>","DOI":"10.1111\/imm.12317","type":"journal-article","created":{"date-parts":[[2014,5,14]],"date-time":"2014-05-14T17:15:35Z","timestamp":1400087735000},"page":"520-530","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Evaluation of intra\u2010 and extra\u2010epithelial secretory IgA in chlamydial infections"],"prefix":"10.1111","volume":"143","author":[{"given":"Charles W.","family":"Armitage","sequence":"first","affiliation":[{"name":"Institute of Health and Biomedical Innovation Queensland University of Technology Kelvin Grove Qld Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Connor P.","family":"O'Meara","sequence":"additional","affiliation":[{"name":"Institute of Health and Biomedical Innovation Queensland University of Technology Kelvin Grove Qld Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marina C. G.","family":"Harvie","sequence":"additional","affiliation":[{"name":"Institute of Health and Biomedical Innovation Queensland University of Technology Kelvin Grove Qld Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Timms","sequence":"additional","affiliation":[{"name":"Institute of Health and Biomedical Innovation Queensland University of Technology Kelvin Grove Qld Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Odilia L.","family":"Wijburg","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology The University of Melbourne Melbourne Vic. 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