{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T08:49:27Z","timestamp":1777538967800,"version":"3.51.4"},"reference-count":59,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2015,4,15]],"date-time":"2015-04-15T00:00:00Z","timestamp":1429056000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Karolinska Institutet and Swedish Research Foundation"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunology"],"published-print":{"date-parts":[[2015,7]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>A high content peptide microarray containing the entire influenza A virus [A\/California\/08\/2009(H1N1)] proteome and haemagglutinin proteins from 12 other influenza A subtypes, including the haemagglutinin from the [A\/South Carolina\/1\/1918(H1N1)] strain, was used to gauge serum IgG epitope signatures before and after Pandemrix<jats:sup>\u00ae<\/jats:sup> vaccination or H1N1 infection in a Swedish cohort during the pandemic influenza season 2009. A very narrow pattern of pandemic flu\u2010specific IgG epitope recognition was observed in the serum from individuals who later contracted H1N1 infection. Moreover, the pandemic influenza infection generated IgG reactivity to two adjacent epitopes of the neuraminidase protein. The differential serum IgG recognition was focused on haemagglutinin 1 (H1) and restricted to classical antigenic sites (Cb) in both the vaccinated controls and individuals with flu infections. We further identified a novel epitope <jats:styled-content style=\"fixed-case\">VEPGDKITFEATGNL<\/jats:styled-content> on the Ca antigenic site (251\u2013265) of the pandemic flu haemagglutinin, which was exclusively recognized in serum from individuals with previous vaccinations and never in serum from individuals with H1N1 infection (confirmed by <jats:styled-content style=\"fixed-case\">RNA PCR<\/jats:styled-content> analysis from nasal swabs). This epitope was mapped to the receptor\u2010binding domain of the influenza haemagglutinin and could serve as a correlate of immune protection in the context of pandemic flu. The study shows that unbiased epitope mapping using peptide microarray technology leads to the identification of biologically and clinically relevant target structures. Most significantly an H1N1 infection induced a different footprint of IgG epitope recognition patterns compared with the pandemic H1N1 vaccine.<\/jats:p>","DOI":"10.1111\/imm.12448","type":"journal-article","created":{"date-parts":[[2015,2,2]],"date-time":"2015-02-02T04:18:46Z","timestamp":1422850726000},"page":"357-366","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["H1N1 viral proteome peptide microarray predicts individuals at risk for H1N1 infection and segregates infection versus Pandemrix<sup>\u00ae<\/sup> vaccination"],"prefix":"10.1111","volume":"145","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8462-3552","authenticated-orcid":false,"given":"Aditya","family":"Ambati","sequence":"first","affiliation":[{"name":"Therapeutic Immunology Unit Department of Laboratory Medicine Karolinska Institutet Stockholm Sweden"},{"name":"Centre for Allogeneic Stem Cell Transplantation (CAST) Karolinska University Hospital Stockholm Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Davide","family":"Valentini","sequence":"additional","affiliation":[{"name":"Therapeutic Immunology Unit Department of Laboratory Medicine Karolinska Institutet Stockholm Sweden"},{"name":"Centre for Allogeneic Stem Cell Transplantation (CAST) Karolinska University Hospital Stockholm Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emanuele","family":"Montomoli","sequence":"additional","affiliation":[{"name":"Department of Molecular and Developmental Medicine University of Siena Siena Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guilia","family":"Lapini","sequence":"additional","affiliation":[{"name":"VisMederi srl Enterprise in Life Science Siena Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabrizio","family":"Biuso","sequence":"additional","affiliation":[{"name":"VisMederi srl Enterprise in Life Science Siena Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Holger","family":"Wenschuh","sequence":"additional","affiliation":[{"name":"JPT Peptide Technologies Berlin Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabelle","family":"Magalhaes","sequence":"additional","affiliation":[{"name":"Therapeutic Immunology Unit Department of Laboratory Medicine Karolinska Institutet Stockholm Sweden"},{"name":"Centre for Allogeneic Stem Cell Transplantation (CAST) Karolinska University Hospital Stockholm Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Markus","family":"Maeurer","sequence":"additional","affiliation":[{"name":"Therapeutic Immunology Unit Department of Laboratory Medicine Karolinska Institutet Stockholm Sweden"},{"name":"Centre for Allogeneic Stem Cell Transplantation (CAST) Karolinska University Hospital Stockholm Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,4,15]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0903810"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1176225"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature08157"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200939911"},{"key":"e_1_2_9_6_1","volume-title":"The 2009 A(H1N1) Pandemic in Europe","year":"2010"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0906453"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc0907256"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature08260"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0911580106"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(82)90135-0"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.69.1.531"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02184-09"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.R110.129809"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.37.100183.002525"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0003840"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0089648"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00262-002-0320-4"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.20094"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.5.1914"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.12.6919"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1089\/aid.2012.0103"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0019-2791(71)90454-X"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/imm.12323"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/imm.12213"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10654-010-9521-x"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1586\/eci.10.62"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-14-319"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2007.07.015"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2008.12.004"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2010.08.007"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1186430"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01412-10"},{"key":"e_1_2_9_34_1","unstructured":"Jmol: an open\u2010source Java viewer for chemical structures in 3D. URLhttp:\/\/www.jmol.org\/[accessed on 20 January 2014]"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.4.937-943.1999"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.091062498"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2004-5-10-r80"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00212-10"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0058810"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.13-0151"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid1511.090685"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.181.6.4168"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200839129"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1003057"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0811271106"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/0161-5890(92)90041-U"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa066092"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00150-11"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.63.2.326"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197206222862502"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2011.410"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.63.5.669"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3006366"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1038\/289373a0"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20130212"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1244730"},{"key":"e_1_2_9_57_1","doi-asserted-by":"crossref","first-page":"4347","DOI":"10.1128\/jvi.71.6.4347-4355.1997","article-title":"A pulmonary influenza virus infection in SCID mice can be cured by treatment with hemagglutinin\u2010specific antibodies that display very low virus\u2010neutralizing activity in vitro","volume":"71","author":"Mozdzanowska K","year":"1997","journal-title":"J Virol"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.3.1369-1378.2002"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2262"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00081-12"}],"container-title":["Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fimm.12448","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/imm.12448","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T22:50:57Z","timestamp":1696287057000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/imm.12448"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,4,15]]},"references-count":59,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2015,7]]}},"alternative-id":["10.1111\/imm.12448"],"URL":"https:\/\/doi.org\/10.1111\/imm.12448","archive":["Portico"],"relation":{},"ISSN":["0019-2805","1365-2567"],"issn-type":[{"value":"0019-2805","type":"print"},{"value":"1365-2567","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,4,15]]},"assertion":[{"value":"2014-09-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-01-26","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-04-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}