{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:24:00Z","timestamp":1792787040747,"version":"4.5.0"},"reference-count":33,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,5,7]],"date-time":"2015-05-07T00:00:00Z","timestamp":1430956800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"crossref","award":["R01 GM-040586"],"award-info":[{"award-number":["R01 GM-040586"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"crossref","award":["R01 GM-097531"],"award-info":[{"award-number":["R01 GM-097531"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Ruth Kirschstein National Research Service Award","award":["T32 GM-008721"],"award-info":[{"award-number":["T32 GM-008721"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunology"],"published-print":{"date-parts":[[2015,6]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Over one million newborns die annually from sepsis with the highest mortality in premature and low\u2010birthweight infants. The inflammasome plays a central role in the regulation of innate immunity and inflammation, and is presumed to be involved in protective immunity, in large part through the caspase\u20101\u2010dependent activation of interleukin\u20101\n                    <jats:italic>\u03b2<\/jats:italic>\n                    (IL\u20101\n                    <jats:italic>\u03b2<\/jats:italic>\n                    ) and IL\u201018. Studies in endotoxic shock, however, suggest that endogenous caspase\u20101 activity and the inflammasome contribute to mortality primarily by promoting excessive systemic inflammatory responses. We examined whether caspase\u20101 and the inflammasome also regulate neonatal inflammation, host protective immunity and myelopoiesis during polymicrobial sepsis. Neonatal (5\u20137\u00a0days) C57BL\/6 and caspase\u20101\/11\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice underwent a low\u2010lethality caecal slurry model of intra\u2010abdominal sepsis (LD\n                    <jats:sub>25\u201345<\/jats:sub>\n                    ). Ablation of caspase\u20101\/11, but not apoptosis\u2010associated speck\u2010like protein containing a CARD domain or nucleotide\u2010binding oligomerization domain\u2010like receptor protein 3 (NLRP3), improved neonatal survival following septic challenge compared with wild\u2010type mice (\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0&lt;\u00a00\u00b7001), with decreased concentrations of inflammatory cytokines in the serum and peritoneum. Surprisingly, caspase\u20101\/11\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    neonates also exhibited increased bone marrow and splenic haematopoietic stem cell expansion (\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0&lt;\u00a00\u00b7001), and increased concentrations of granulocyte and macrophage colony\u2010stimulating factors in the peritoneum (\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0&lt;\u00a00\u00b7001) after sepsis. Ablation of caspase\u20101\/11 signalling was also associated with increased recruitment of peritoneal macrophages and neutrophils (\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0&lt;\u00a00\u00b7001), increased phagocytosis by neutrophils (\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0=\u00a00\u00b7003), and decreased bacterial colonization (\n                    <jats:italic>P<\/jats:italic>\n                    \u00a0=\u00a00\u00b702) in the peritoneum. These findings suggest that endogenous caspase\u20101\/11 activity, independent of the NLRP3 inflammasome, not only promotes the magnitude of the inflammatory response, but also suppresses protective immunity in the neonate, so contributing to innate immune dysfunction and poor survival in neonatal sepsis.\n                  <\/jats:p>","DOI":"10.1111\/imm.12450","type":"journal-article","created":{"date-parts":[[2015,2,13]],"date-time":"2015-02-13T06:10:14Z","timestamp":1423807814000},"page":"300-311","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["Improved emergency myelopoiesis and survival in neonatal sepsis by caspase\u20101\/11 ablation"],"prefix":"10.1111","volume":"145","author":[{"given":"Lori F.","family":"Gentile","sequence":"first","affiliation":[{"name":"Department of Surgery University of Florida College of Medicine Gainesville FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela L.","family":"Cuenca","sequence":"additional","affiliation":[{"name":"Department of Surgery University of Florida College of Medicine Gainesville FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex G.","family":"Cuenca","sequence":"additional","affiliation":[{"name":"Department of Surgery University of Florida College of Medicine Gainesville FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dina C.","family":"Nacionales","sequence":"additional","affiliation":[{"name":"Department of Surgery University of Florida College of Medicine Gainesville FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo","family":"Ungaro","sequence":"additional","affiliation":[{"name":"Department of Surgery University of Florida College of Medicine Gainesville FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip\u00a0A.","family":"Efron","sequence":"additional","affiliation":[{"name":"Department of Surgery University of Florida College of Medicine Gainesville FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lyle L.","family":"Moldawer","sequence":"additional","affiliation":[{"name":"Department of Surgery University of Florida College of Medicine Gainesville FL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shawn D.","family":"Larson","sequence":"additional","affiliation":[{"name":"Department of Surgery University of Florida College of Medicine Gainesville FL 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