{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:24:00Z","timestamp":1792787040912,"version":"4.5.0"},"reference-count":39,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,4,21]],"date-time":"2015-04-21T00:00:00Z","timestamp":1429574400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"State Megaproject for Infectious Disease Research of China","award":["2014ZX10004001-002"],"award-info":[{"award-number":["2014ZX10004001-002"]}]},{"name":"State Megaproject for Infectious Disease Research of China","award":["2013ZX10004601"],"award-info":[{"award-number":["2013ZX10004601"]}]},{"name":"State Megaproject for Infectious Disease Research of China","award":["2011ZX10004-803-006"],"award-info":[{"award-number":["2011ZX10004-803-006"]}]},{"name":"National 973 Program of China","award":["2011CB504704"],"award-info":[{"award-number":["2011CB504704"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunology"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    An ideal vaccine against mucosal pathogens such as Middle East respiratory syndrome coronavirus (MERS\u2010CoV) should confer sustained, protective immunity at both systemic and mucosal levels. Here, we evaluated the\n                    <jats:italic>in\u00a0vivo<\/jats:italic>\n                    systemic and mucosal antigen\u2010specific immune responses induced by a single intramuscular or intragastric administration of recombinant adenoviral type 5 (Ad5) or type 41 (Ad41) \u2010based vaccines expressing the MERS\u2010CoV spike (S) protein. Intragastric administration of either Ad5\u2010S or Ad41\u2010S induced antigen\u2010specific IgG and neutralizing antibody in serum; however, antigen\u2010specific T\u2010cell responses were not detected. In contrast, after a single intramuscular dose of Ad5\u2010S or Ad41\u2010S, functional antigen\u2010specific T\u2010cell responses were elicited in the spleen and pulmonary lymphocytes of the mice, which persisted for several months. Both\n                    <jats:styled-content style=\"fixed-case\">rA<\/jats:styled-content>\n                    d\u2010based vaccines administered intramuscularly induced systemic humoral immune responses (neutralizing IgG antibodies). Our results show that a single dose of Ad5\u2010S\u2010 or Ad41\u2010S\u2010based vaccines represents an appealing strategy for the control of MERS\u2010CoV infection and transmission.\n                  <\/jats:p>","DOI":"10.1111\/imm.12462","type":"journal-article","created":{"date-parts":[[2015,3,12]],"date-time":"2015-03-12T03:45:18Z","timestamp":1426131918000},"page":"476-484","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":104,"title":["Systemic and mucosal immunity in mice elicited by a single immunization with human adenovirus type 5 or 41 vector\u2010based vaccines carrying the spike protein of Middle East respiratory syndrome coronavirus"],"prefix":"10.1111","volume":"145","author":[{"given":"Xiaojuan","family":"Guo","sequence":"first","affiliation":[{"name":"Key Laboratory of Medical Virology Ministry of Health; National Institute for Viral Disease Control and Prevention China CDC Beijing 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Virology Ministry of Health; National Institute for Viral Disease Control and Prevention China CDC Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjie","family":"Tan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Medical Virology Ministry of Health; National Institute for Viral Disease Control and Prevention China CDC Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,4,21]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1211721"},{"key":"e_1_2_7_3_1","doi-asserted-by":"crossref","first-page":"20290","DOI":"10.2807\/ese.17.40.20290-en","article-title":"Severe respiratory illness caused by a novel coronavirus, in a patient transferred to the United Kingdom from the Middle East","volume":"17","author":"Bermingham A","year":"2012","journal-title":"Euro 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