{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T17:52:39Z","timestamp":1777657959063,"version":"3.51.4"},"reference-count":27,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2015,6,29]],"date-time":"2015-06-29T00:00:00Z","timestamp":1435536000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["813220437"],"award-info":[{"award-number":["813220437"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunology"],"published-print":{"date-parts":[[2015,9]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Leukotriene B<jats:sub>4<\/jats:sub> (<jats:styled-content style=\"fixed-case\">LTB<\/jats:styled-content><jats:sub>4<\/jats:sub>) is a lipid mediator that is rapidly generated in inflammatory sites, and its functional receptor, <jats:styled-content style=\"fixed-case\">BLT<\/jats:styled-content>1, is mostly expressed on immune cells. Contact dermatitis is a common inflammatory skin disease characterized by skin oedema and abundant inflammatory infiltrates, primarily including neutrophils and <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>8<jats:sup>+<\/jats:sup> T cells. The role of the <jats:styled-content style=\"fixed-case\">LTB<\/jats:styled-content><jats:sub>4<\/jats:sub>\u2013<jats:styled-content style=\"fixed-case\">BLT<\/jats:styled-content>1 axis in contact dermatitis remains largely unknown. In this study, we found up\u2010regulated gene expression of 5\u2010lipoxygenase and leukotriene A<jats:sub>4<\/jats:sub> hydrolase, two critical enzymes for <jats:styled-content style=\"fixed-case\">LTB<\/jats:styled-content><jats:sub>4<\/jats:sub> synthesis, <jats:styled-content style=\"fixed-case\">BLT<\/jats:styled-content>1 and elevated <jats:styled-content style=\"fixed-case\">LTB<\/jats:styled-content><jats:sub>4<\/jats:sub> levels in skin lesions of oxazolone (<jats:styled-content style=\"fixed-case\">OXA<\/jats:styled-content>)\u2010induced contact dermatitis. <jats:styled-content style=\"fixed-case\">BLT<\/jats:styled-content>1 deficiency or blockade of <jats:styled-content style=\"fixed-case\">LTB<\/jats:styled-content><jats:sub>4<\/jats:sub> and <jats:styled-content style=\"fixed-case\">BLT<\/jats:styled-content>1 by the antagonists, bestatin and U\u201075302, respectively, in the elicitation phase caused significant decreases in ear swelling and skin\u2010infiltrating neutrophils and <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>8<jats:sup>+<\/jats:sup> T cells, which was accompanied by significantly reduced skin expression of <jats:styled-content style=\"fixed-case\">CXCL<\/jats:styled-content>1, <jats:styled-content style=\"fixed-case\">CXCL<\/jats:styled-content>2, interferon\u2010<jats:italic>\u03b3<\/jats:italic> and interleukin\u20101<jats:italic>\u03b2<\/jats:italic>. Furthermore, neutrophil depletion during the elicitation phase of <jats:styled-content style=\"fixed-case\">OXA<\/jats:styled-content>\u2010induced contact dermatitis also caused significant decreases in ear swelling and <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>8<jats:sup>+<\/jats:sup> T\u2010cell infiltration accompanied by significantly decreased <jats:styled-content style=\"fixed-case\">LTB<\/jats:styled-content><jats:sub>4<\/jats:sub> synthesis and gene expression of <jats:styled-content style=\"fixed-case\">CXCL<\/jats:styled-content>2, interferon\u2010<jats:italic>\u03b3<\/jats:italic> and interleukin\u20101<jats:italic>\u03b2<\/jats:italic>. Importantly, subcutaneous injection of exogenous <jats:styled-content style=\"fixed-case\">LTB<\/jats:styled-content><jats:sub>4<\/jats:sub> restored the skin infiltration of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>8<jats:sup>+<\/jats:sup> T cells in neutrophil\u2010depleted mice following <jats:styled-content style=\"fixed-case\">OXA<\/jats:styled-content> challenge. Collectively, our results demonstrate that the <jats:styled-content style=\"fixed-case\">LTB<\/jats:styled-content><jats:sub>4<\/jats:sub>\u2013<jats:styled-content style=\"fixed-case\">BLT<\/jats:styled-content>1 axis contributes to <jats:styled-content style=\"fixed-case\">OXA<\/jats:styled-content>\u2010induced contact dermatitis by mediating skin recruitment of neutrophils, which are a major source of <jats:styled-content style=\"fixed-case\">LTB<\/jats:styled-content><jats:sub>4<\/jats:sub> that sequentially direct <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>8<jats:sup>+<\/jats:sup> T\u2010cell homing to <jats:styled-content style=\"fixed-case\">OXA<\/jats:styled-content>\u2010challenged skin. Hence, <jats:styled-content style=\"fixed-case\">LTB<\/jats:styled-content><jats:sub>4<\/jats:sub> and <jats:styled-content style=\"fixed-case\">BLT<\/jats:styled-content>1 could be potential therapeutic targets for the treatment of contact dermatitis.<\/jats:p>","DOI":"10.1111\/imm.12478","type":"journal-article","created":{"date-parts":[[2015,5,9]],"date-time":"2015-05-09T08:06:30Z","timestamp":1431158790000},"page":"50-58","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Leukotriene <scp>B<\/scp><sub>4<\/sub>\u2014leukotriene <scp>B<\/scp><sub>4<\/sub> receptor axis promotes oxazolone\u2010induced contact dermatitis by directing skin homing of neutrophils and <scp>CD<\/scp>8<sup>+<\/sup> T cells"],"prefix":"10.1111","volume":"146","author":[{"given":"Jiaoyan","family":"Lv","sequence":"first","affiliation":[{"name":"Department of Immunology School of Basic Medical Sciences Fudan University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linlin","family":"Zou","sequence":"additional","affiliation":[{"name":"Department of Immunology School of Basic Medical Sciences Fudan University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lina","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Immunology School of Basic Medical Sciences Fudan University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Immunology School of Basic Medical Sciences Fudan University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yingluo","family":"Xiong","sequence":"additional","affiliation":[{"name":"Department of Immunology School of Basic Medical Sciences Fudan University Shanghai 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