{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T17:12:16Z","timestamp":1772989936713,"version":"3.50.1"},"reference-count":46,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2015,6,25]],"date-time":"2015-06-25T00:00:00Z","timestamp":1435190400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Immunology"],"published-print":{"date-parts":[[2015,9]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Natural killer T cells (<jats:styled-content style=\"fixed-case\">NKT<\/jats:styled-content> cells) are comprised of several subsets. However, the possible differences in their developmental mechanisms have not been fully investigated. To evaluate the dependence of some <jats:styled-content style=\"fixed-case\">NKT<\/jats:styled-content> subpopulations on nuclear factor\u2010<jats:italic>\u03ba<\/jats:italic>B\u2010inducing kinase (<jats:styled-content style=\"fixed-case\">NIK<\/jats:styled-content>) for their generation, we analysed the differentiation of <jats:styled-content style=\"fixed-case\">NKT<\/jats:styled-content> cells, dividing them into subsets in various tissues of <jats:italic>alymphoplasia<\/jats:italic> (<jats:italic>aly<\/jats:italic>\/<jats:italic>aly<\/jats:italic>), a mutant mouse strain that lacks functional <jats:styled-content style=\"fixed-case\">NIK<\/jats:styled-content>. The results indicated that the efficient differentiation of both invariant <jats:styled-content style=\"fixed-case\">NKT<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">iNKT<\/jats:styled-content>) and non\u2010<jats:styled-content style=\"fixed-case\">iNKT<\/jats:styled-content> cells relied on <jats:styled-content style=\"fixed-case\">NIK<\/jats:styled-content> expression in non\u2010haematopoietic cells; however, the dependence of non\u2010<jats:styled-content style=\"fixed-case\">iNKT<\/jats:styled-content> cells was lower than that of <jats:styled-content style=\"fixed-case\">iNKT<\/jats:styled-content> cells. Especially, the differentiation of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>8<jats:sup>+<\/jats:sup> non\u2010<jats:styled-content style=\"fixed-case\">iNKT<\/jats:styled-content> cells was markedly resistant to the <jats:italic>aly<\/jats:italic> mutation. The proportion of two other <jats:styled-content style=\"fixed-case\">NKT<\/jats:styled-content> cell subsets, <jats:styled-content style=\"fixed-case\">NK<\/jats:styled-content>1.1<jats:sup>+<\/jats:sup> <jats:italic>\u03b3\u03b4<\/jats:italic> T cells and <jats:styled-content style=\"fixed-case\">NK<\/jats:styled-content>1.1<jats:sup>\u2212<\/jats:sup> <jats:styled-content style=\"fixed-case\">iNKT<\/jats:styled-content> cells, was also significantly reduced in <jats:italic>aly<\/jats:italic>\/<jats:italic>aly<\/jats:italic> mice, and this defect in their development was reversed in wild\u2010type host mice given <jats:italic>aly<\/jats:italic>\/<jats:italic>aly<\/jats:italic> bone marrow cells. In exerting effector functions, <jats:styled-content style=\"fixed-case\">NIK<\/jats:styled-content> in <jats:styled-content style=\"fixed-case\">NKT<\/jats:styled-content>\u2010<jats:italic>\u03b1\u03b2<\/jats:italic> cells appeared dispensable, as <jats:styled-content style=\"fixed-case\">NIK<\/jats:styled-content>\u2010deficient <jats:styled-content style=\"fixed-case\">NKT<\/jats:styled-content>\u2010<jats:italic>\u03b1\u03b2<\/jats:italic> cells could secrete interleukin\u20104 or interferon\u2010<jats:italic>\u03b3<\/jats:italic> and exhibit cytolytic activity at a level comparable to that of <jats:italic>aly\/+ <\/jats:italic><jats:styled-content style=\"fixed-case\">NKT<\/jats:styled-content>\u2010<jats:italic>\u03b1\u03b2<\/jats:italic> cells. Collectively, these results imply that the <jats:styled-content style=\"fixed-case\">NIK<\/jats:styled-content> in thymic stroma may be critically involved in the differentiation of most <jats:styled-content style=\"fixed-case\">NKT<\/jats:styled-content> cell subsets (although the level of <jats:styled-content style=\"fixed-case\">NIK<\/jats:styled-content> dependence may vary among the subsets), and also that <jats:styled-content style=\"fixed-case\">NIK<\/jats:styled-content> in <jats:styled-content style=\"fixed-case\">NKT<\/jats:styled-content>\u2010<jats:italic>\u03b1\u03b2<\/jats:italic> cells may be dispensable for their effector function.<\/jats:p>","DOI":"10.1111\/imm.12484","type":"journal-article","created":{"date-parts":[[2015,5,19]],"date-time":"2015-05-19T05:54:48Z","timestamp":1432014888000},"page":"89-99","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Differential dependence on nuclear factor\u2010<i>\u03ba<\/i>B\u2010inducing kinase among natural killer <scp>T<\/scp>\u2010cell subsets in their development"],"prefix":"10.1111","volume":"146","author":[{"given":"Haruka","family":"Noma","sequence":"first","affiliation":[{"name":"Department of Immunology Kitasato University School of Medicine Sagamihara Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koji","family":"Eshima","sequence":"additional","affiliation":[{"name":"Department of Immunology Kitasato University School of Medicine Sagamihara Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masashi","family":"Satoh","sequence":"additional","affiliation":[{"name":"Department of Immunology Kitasato University School of Medicine Sagamihara Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuya","family":"Iwabuchi","sequence":"additional","affiliation":[{"name":"Department of Immunology Kitasato University School of Medicine Sagamihara Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,6,25]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.25.022106.141711"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/imm.12247"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1001255"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2731"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0806472105"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1841"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00262-013-1509-4"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/385540a0"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2011.01088.x"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830240224"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/8780"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.6.2472"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20022234"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20030141"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1006\/cimm.2000.1723"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni1351"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-12-192914"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI44943"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.169.3.1151"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.165.2.804"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20110128"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/imm.12186"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature01433"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90029-C"},{"key":"e_1_2_8_26_1","first-page":"e51780","article-title":"Isolation, 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