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H\u2010Ficolin, an innate immune opsonin, is produced by type <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> alveolar epithelial cells and could participate in lung defences against infections. Here, we used the human type <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> alveolar epithelial cell line, A549, to determine the involvement of H\u2010ficolin in fungal defence. Additionally, we investigated the presence of H\u2010ficolin in bronchoalveolar lavage fluid from transplant patients during pneumonia. H\u2010Ficolin exhibited demonstrable binding to <jats:italic>A.\u00a0fumigatus<\/jats:italic> conidia via <jats:sc>l<\/jats:sc>\u2010fucose, <jats:sc>d<\/jats:sc>\u2010mannose and <jats:italic>N<\/jats:italic>\u2010acetylglucosamine residues in a calcium\u2010 and <jats:styled-content style=\"fixed-case\">pH<\/jats:styled-content>\u2010dependent manner. Moreover, recognition led to lectin complement pathway activation and enhanced fungal association with A549 cells. Following recognition, H\u2010ficolin opsonization manifested an increase in interleukin\u20108 production from A549 cells, which involved activation of the intracellular signalling pathways mitogen\u2010activated protein kinase <jats:styled-content style=\"fixed-case\">MAPK<\/jats:styled-content> kinase 1\/2, p38 <jats:styled-content style=\"fixed-case\">MAPK<\/jats:styled-content> and c\u2010<jats:styled-content style=\"fixed-case\">J<\/jats:styled-content>un <jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>\u2010terminal kinase. Finally, H\u2010ficolin concentrations were significantly higher in bronchoalveolar lavage fluid of patients with lung infections compared with control subjects (<jats:italic>n<\/jats:italic>\u00a0=\u00a016; <jats:italic>P<\/jats:italic>\u00a0=\u00a00\u00b700726). Receiver operating characteristics curve analysis further highlighted the potential of H\u2010ficolin as a diagnostic marker for lung infection (area under the curve\u00a0=\u00a00\u00b777; <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00\u00b70001). Hence, H\u2010ficolin participates in <jats:italic>A.\u00a0fumigatus<\/jats:italic> defence through the activation of the lectin complement pathway, enhanced fungus\u2013host interactions and modulated immune responses.<\/jats:p>","DOI":"10.1111\/imm.12501","type":"journal-article","created":{"date-parts":[[2015,7,1]],"date-time":"2015-07-01T15:09:52Z","timestamp":1435763392000},"page":"281-291","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":44,"title":["H\u2010ficolin binds <i><scp>A<\/scp>spergillus fumigatus<\/i> leading to activation of the lectin complement pathway and modulation of lung epithelial immune responses"],"prefix":"10.1111","volume":"146","author":[{"given":"Stefan","family":"Bidula","sequence":"first","affiliation":[{"name":"Biomedical Research Centre Norwich Medical School University of East Anglia Norwich UK"},{"name":"Aberdeen Fungal Group School of Medical Sciences Institute of Medical Sciences University of Aberdeen Aberdeen UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darren W.","family":"Sexton","sequence":"additional","affiliation":[{"name":"Biomedical Research Centre Norwich Medical School University of East Anglia Norwich UK"},{"name":"School of Pharmacy and Biomolecular Science Liverpool John Moores University Liverpool UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Yates","sequence":"additional","affiliation":[{"name":"Biomedical Research Centre Norwich Medical School University of East Anglia Norwich UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alireza","family":"Abdolrasouli","sequence":"additional","affiliation":[{"name":"Section\u00a0of Infectious Diseases and Immunity Imperial College London London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anand","family":"Shah","sequence":"additional","affiliation":[{"name":"Section\u00a0of Infectious Diseases and Immunity Imperial College London London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Russell","family":"Wallis","sequence":"additional","affiliation":[{"name":"Departments of Infection, Immunity and Inflammation and Biochemistry University of Leicester Leicester UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Reed","sequence":"additional","affiliation":[{"name":"Department of Lung Transplantation Harefield Hospital Middlesex UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darius","family":"Armstrong\u2010James","sequence":"additional","affiliation":[{"name":"Section\u00a0of Infectious Diseases and Immunity Imperial College London London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silke","family":"Schelenz","sequence":"additional","affiliation":[{"name":"Biomedical Research Centre Norwich Medical School University of East Anglia Norwich UK"},{"name":"Department of Microbiology Royal Brompton Hospital London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,8,24]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2012.08.003"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13054-014-0722-7"},{"key":"e_1_2_8_4_1","unstructured":"NHS. 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